Fertility restoration in three CMS systems of eggplant by the Rf genes of each other's systems and their SCAR marker

Khan, Md. Mizanur Rahim; Hasnunnahar, Mst.; Iwayoshi, Masaki; Isshiki, Shiro

doi:10.1016/j.scienta.2014.04.013

Cited by 13 publications

(11 citation statements)

References 27 publications

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“…Obtaining interspecific hybrids between scarlet and/or gboma eggplants and common eggplant is a key step on the way of reciprocal improvement of these three crops, as backcrosses of the hybrids to both species have been obtained (Toppino et al, 2008;Prohens et al, 2012;Khan et al, 2014;Rotino et al, 2014). In the case of gboma eggplant, interspecific hybrids with common eggplant are highly sterile (Bletsos et al, 2004;Khan et al, 2013) and no backcross generations to any of the parents have been reported, which limits the use of interspecific hybridization between both eggplants for breeding new gboma or common eggplant varieties.…”

Section: Composition Of Interspecific Hybrids Between Scarlet and Commentioning

confidence: 99%

“…Therefore, the study of the diversity for chemical and nutritional composition in different types of cultivars will be useful for the selection and breeding of improved varieties of scarlet and gboma eggplant. Also, given the feasibility of reciprocal introgression between scarlet and common eggplant (Toppino et al, 2008;Prohens et al, 2012;Khan et al, 2014;Rotino et al, 2014), the evaluation of interspecific hybrids of these two cultivated eggplants can also provide information of interest for breeding.…”

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confidence: 99%

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Diversity in composition of scarlet (S. aethiopicum) and gboma (S. macrocarpon) eggplants and of interspecific hybrids between S. aethiopicum and common eggplant (S. melongena)

José

Plazas

Sánchez-Mata

et al. 2016

Journal of Food Composition and Analysis

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Section: Composition Of Interspecific Hybrids Between Scarlet and Commentioning

confidence: 99%

mentioning

confidence: 99%

Diversity in composition of scarlet (S. aethiopicum) and gboma (S. macrocarpon) eggplants and of interspecific hybrids between S. aethiopicum and common eggplant (S. melongena)

José

Plazas

Sánchez-Mata

et al. 2016

Journal of Food Composition and Analysis

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“…This process may be facilitated by the development of molecular markers linked to the Rf-genes. That was demonstrated to be applicable for the Rf-genes of the pollen non-formation eggplant CMS lines (Khan, Hasnunnahar, Iwayoshi, & Isshiki, 2014).…”

Section: Discussmentioning

confidence: 99%

Development and Fertility Restoration of CMS Eggplant Lines Carrying the Cytoplasm of Solanum violaceum

Krommydas¹,

Tzikalios²,

Madesis³

et al. 2016

JAS

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A functional cytoplasmic male sterile (CMS) eggplant line carrying the cytoplasm of Solanum violaceum was developed in the past, but the fertility restoring genes (Rf-genes) were not identified. This work aimed to produce the CMS lines of three Hellenic eggplant cultivars (viz., 'Langada', 'Emi' and 'Tsakoniki') using the cytoplasm of S. violaceum and study the inheritance of the Rf-genes. The respective CMS eggplant lines were developed by the backcross method and examined for their fertility parameters. The results demonstrated that female fertility was not affected by the cytoplasm of S. violaceum. In contrast, the occurrence of three male fertility phenotypes (male sterile, male fertile and potentially male fertile) indicated that male fertility was affected by nuclear/cytoplasmic interactions. Male sterile plants were characterized by indehiscent anthers, low pollen viability and abnormal anther morphology. Male fertile plants formed dehiscent anthers with high pollen viability and normal morphology. Potentially male fertile plants initially formed dehiscent anthers, but in later stages formed exclusively indehiscent anthers. Male fertile plants were obtained in the advanced backcross populations of CMS 'Tsakoniki', but not in CMS 'Langada' and CMS 'Emi'. The genetic analysis of fertility restoration indicated that male fertility in the genetic background of cv. 'Tsakoniki' is controlled by one essential genetic locus, affected by a secondary modifying locus. Molecular analysis of cp-DNA and mt-DNA in the CMS lines indicated maternal inheritance of the cytoplasm organelles. Our findings demonstrate that the genotype of the eggplant parent can affect the expression of CMS as well as fertility restoration.

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“…An Rf gene has not been discovered yet in anther indehiscent male sterility systems of eggplant developed by utilizing the cytoplasms of S. kurzii, S. violaceum, and S. virginianum. However, Rf genes were found in the CMS systems with the cytoplasms of S. aethiopicum Aculeatum Group, S. anguivi, and S. grandifolium (Khan et al, 2014). Fertility restoration ability by the Rf genes of each other's systems in three pollen non-formation-type CMS systems of eggplant was evaluated.…”

Section: Fertility Restoration In the Cms Systems Of Eggplant By The mentioning

confidence: 99%

“…DNA markers linked to the Rf gene were screened from three pollen non-formation-type CMS systems of eggplant with the cytoplasms of S. aethiopicum Aculeatum Group, S. anguivi, and S. grandifolium by bulked segregant analysis using random amplified polymorphic DNA (RAPD) (Khan et al, 2014). Aliquots of DNA from 10 arbitrarily selected MF and MS plants from backcross progeny were bulked separately for each CMS system.…”

Section: Development Of Scar Marker Linked To Rf Genementioning

confidence: 99%

Cytoplasmic Male Sterility in Eggplant

Khan

Isshiki

2016

The Hortic J

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Cytoplasmic male sterility (CMS) is a useful system to produce hybrid seeds in a variety of crop species. In eggplant, CMS systems were developed utilizing the cytoplasms of six wild Solanum species by repeated backcrossings. These CMS systems were classified into two types. The first one is anther indehiscent-type sterility in the CMS systems of eggplant with the cytoplasms of Solanum kurzii Brace & Prain, S. violaceum Ort., and S. virginianum L., in which anther contains normal pollen but does not open to release. The second one is pollen non-formation-type sterility in the CMS systems of eggplant with the cytoplasms of S. aethiopicum Aculeatum Group, S. anguivi Lam., and S. grandifolium C.V. Morton, in which anther of the male-sterile lines is completely devoid of pollen. Both types of sterility system were characterized by investigating pollen and seed fertility. Furthermore, in pollen non-formation CMS systems, two independent dominant fertility restorer (Rf) genes were discovered and a sequenced characterized amplified region (SCAR) marker tightly linked to these genes was developed.

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Fertility restoration in three CMS systems of eggplant by the Rf genes of each other's systems and their SCAR marker

Cited by 13 publications

References 27 publications

Diversity in composition of scarlet (S. aethiopicum) and gboma (S. macrocarpon) eggplants and of interspecific hybrids between S. aethiopicum and common eggplant (S. melongena)

Diversity in composition of scarlet (S. aethiopicum) and gboma (S. macrocarpon) eggplants and of interspecific hybrids between S. aethiopicum and common eggplant (S. melongena)

Development and Fertility Restoration of CMS Eggplant Lines Carrying the Cytoplasm of Solanum violaceum

Cytoplasmic Male Sterility in Eggplant

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