Fertilization and early embryo development in the potato

Clarke, Alfred E.

doi:10.1007/bf02879202

Cited by 18 publications

(12 citation statements)

References 6 publications

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“…2A). This transient increase in ScNLE gene expression around 36-42 HAP corresponds exactly to the intense period of basipetal fertilization of the multiple ovules present in the ovary and to the initiation of seed development in some Solanaceous species, including S. chacoense (Clarke 1940;Williams 1955, and our unpublished results). To confirm that the strong increase in ScNLE expression was fertilization-dependent and not only a consequence of pollination, we took advantage of the gametophytic self-incompatibility system present in S. chacoense.…”

Section: Scnle Expression Pattern In Solanum Chacoensementioning

confidence: 64%

“…S. chacoense is self-incompatible and pollination time can therefore be easily controlled. Pollen tubes reach the first ovules in the ovary around 36 h after pollination (HAP) and fertilization is completed for all ovules around 42-48 HAP (Clarke 1940;Williams 1955 and our unpublished observations). The selection screen was initially carried on 2000 clones obtained from a cDNA library of pistils collected 48 HAP.…”

Section: Isolation Of the Nle Gene In Solanum Chacoensementioning

confidence: 74%

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Characterization of the plant Notchless homolog, a WD repeat protein involved in seed development

2006

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We have isolated a plant NOTCHLESS (NLE) homolog from the wild potato species Solanum chacoense Bitt., encoding a WD-repeat containing protein initially characterized as a negative regulator of the Notch receptor in animals. Although no Notch signaling pathway exists in plants, the NLE gene is conserved in animals, plants, and yeast. Overexpression of the plant ScNLE gene in Drosophila similarly affected bristle formation when compared to the overexpression of the endogenous Drosophila NLE gene, suggesting functional conservation. Expression analyses showed that the ScNLE gene was fertilization-induced and primarily expressed in ovules after fertilization, mainly in the integumentary tapetum (endothelium). Significant expression was also detected in the shoot apex. Promoter deletion analysis revealed that the ScNLE promoter had a complex modulatory architecture with both positive, negative, and tissue specific regulatory elements. Transgenic plants with reduced levels of ScNLE transcripts displayed pleitotropic phenotypes including a severe reduction in seed set, consistent with ScNLE gene expression pattern.

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Section: Scnle Expression Pattern In Solanum Chacoensementioning

confidence: 64%

Section: Isolation Of the Nle Gene In Solanum Chacoensementioning

confidence: 74%

Characterization of the plant Notchless homolog, a WD repeat protein involved in seed development

2006

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“…ScMDN1 expression was weaker from 12 to 30 h after pollination (HAP), increased at 42 and 48 HAP, and then decreased to basal levels at 72 HAP. In some Solanaceous species, including S. chacoense (Clarke 1940;Williams 1955;and our unpublished results), the period comprised between 36 and 42 HAP corresponds to the intense basipetal fertilization of the multiple ovules present in the ovary and to initiation of seed development.…”

Section: Scmdn1 Expression Pattern In S Chacoensementioning

confidence: 97%

Underexpression of the plant NOTCHLESS gene, encoding a WD-repeat protein, causes pleitropic phenotype during plant development

Chantha

Matton

2006

Planta

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WD-repeat proteins are involved in a breadth of cellular processes. While the WD-repeat protein encoding gene NOTCHLESS has been involved in the regulation of the Notch signaling pathway in Drosophila, its yeast homolog Rsa4p was shown to participate in 60S ribosomal subunit biogenesis. The plant homolog ScNLE was previously characterized in Solanum chacoense (ScNLE) as being involved in seed development. However, expression data and reduced size of ScNLE underexpressing plants suggested in addition a role during shoot development. We here report the detailed phenotypic characterization of ScNLE underexpressing plants during shoot development. ScNLE was shown to be expressed in actively dividing cells of the shoot apex. Consistent with this, ScNLE underexpression caused pleiotropic defects such as a reduction in aerial organ size, a reduction in some organ numbers, delayed flowering, and an increase in stomatal index. Analysis of adaxial epidermal cells revealed that both cell number and cell size were reduced in mature leaves of ScNLE underexpressing lines. Two-hybrid screens with the Nle domain and the WD-repeat domain of ScNLE allowed the isolation of homologs of yeast MIDASIN and NSA2 genes, the products of which are involved in 60S ribosomal subunit biogenesis in yeast. A ScNLE-GFP chimeric protein was localized in both the cytoplasm and nucleus. These data altogether suggest that ScNLE likely plays a role in 60S ribosomal subunit biogenesis, which is essential for proper cellular growth and proliferation during plant development.

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“…Since pollen tubes reach the embryo sac about 36 h after pollination, and fertilization occurs between 36 and 48 h after pollination in Solanum spp. (Clarke 1940;Williams 1955; and our unpublished results), the increase in ScPIP2a mRNA expression is thus most probably a fertilization-dependant event, and not only triggered by pollen tube growth in the style as observed before for other genes responding to pollination and fertilization (Lantin et al 1999). To ascertain that the ScPIP2a mRNA increase was not due to a late response to pollination, an incompatible self-pollination was performed.…”

Section: Scpip2a Mrna Expression In Mature Tissuesmentioning

confidence: 78%

Characterization of a fertilization-induced and developmentally regulated plasma-membrane aquaporin expressed in reproductive tissues, in the wild potato Solanum chacoense Bitt.

O’Brien

Bertrand

Matton

2002

Planta

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Fertilization triggers a unique and complex developmental program leading to embryogenesis and seed set. Growth and differentiation are accompanied by rapid metabolic changes as well as massive cellular reorganization inside the fertilized ovule. Using differential display to isolate genes involved in pollen-pistil interactions and early fertilization events, we isolated from Solanum chacoense Bitt. a fertilization-induced plasma-membrane intrinsic protein of the PIP2 family that is predominantly expressed in pistil and anthers tissues. Major intrinsic proteins (MIPs) consist of a large family of highly conserved membrane-spanning proteins that are mainly represented in plants by the aquaporins. Aquaporins, mainly of the PIP and TIP type, have been shown in many species to serve as water channels. In S. chacoense the ScPIP2a mRNA is developmentally regulated during anthesis, with mRNA levels gradually decreasing as the pistil reaches maturity. In flowers, strongest expression was observed in elongating styles, in stamens, and transiently in ovules following fertilization. In styles, maximal expression levels correlated with phases of rapid style elongation and with the formation of epidermal papillae. ScPIP2a mRNA was also strongly expressed in developing fruit, consistent with a role in cell expansion during maturation and development.

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Fertilization and early embryo development in the potato

Cited by 18 publications

References 6 publications

Characterization of the plant Notchless homolog, a WD repeat protein involved in seed development

Characterization of the plant Notchless homolog, a WD repeat protein involved in seed development

Underexpression of the plant NOTCHLESS gene, encoding a WD-repeat protein, causes pleitropic phenotype during plant development

Characterization of a fertilization-induced and developmentally regulated plasma-membrane aquaporin expressed in reproductive tissues, in the wild potato Solanum chacoense Bitt.

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