Fertilization outcome could be regulated by binding of oviductal plasminogen to oocytes and by releasing of plasminogen activators during interplay between gametes

Mondéjar, Irene; Grullón, Luis Alberto; García–Vázquez, Francisco Alberto; Romar, Raquel; Coy, Pilar

doi:10.1016/j.fertnstert.2011.11.032

Cited by 34 publications

(46 citation statements)

References 50 publications

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“…Plasminogen is present in the oviductal fluid through the estrous cycle (Mondéjar et al 2012). Plasminogen activators and their main substrate, plasminogen, were also found in the cumulus cell extracellular matrix and oocyte ZP (Roldán-Olarte et al 2005, Mondéjar et al 2012.…”

Section: Proteasesmentioning

confidence: 99%

Oviductal secretion and gamete interaction

Ghersevich¹,

Massa²,

Zumoffen³

2015

Reproduction

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Experimental evidence from the last 30 years supports the fact that the oviduct is involved in the modulation of the reproductive process in eutherian mammals. Oviductal secretion contains molecules that contribute to regulation of gamete function, gamete interaction, and the early stages of embryo development. The oviductal environment would act as a sperm reservoir, maintaining sperm viability, and modulating the subpopulation of spermatozoa that initiates the capacitation process. It could also contribute to prevent the premature acrosome reaction and to reduce polyspermy. Many studies have reported the beneficial effects of the oviductal environment on fertilization and on the first stages of embryo development. Some oviductal factors have been identified in different mammalian species. The effects of oviductal secretion on the reproductive process could be thought to result from the dynamic combined action (inhibitory or stimulatory) of multiple factors present in the oviductal lumen at different stages of the ovulatory cycle and in the presence of gametes or embryos. It could be hypothesized that the absence of a given molecule would not affect fertility as its action could be compensated by another factor with similar functions. However, any alteration in this balance could affect certain events of the reproductive process and could perhaps impair fertility. Thus, the complexity of the reproductive process warrants a continuous research effort to unveil the mechanisms and factors behind its regulation in the oviductal microenvironment.

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Section: Proteasesmentioning

confidence: 99%

Oviductal secretion and gamete interaction

Ghersevich¹,

Massa²,

Zumoffen³

2015

Reproduction

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“…It has been shown that embryo culture in the presence of oviductin increased embryo development in vivo in pigs (McCauley et al, 2003) and sheep (Pradeep et al, 2011). Coy et al (2008Coy et al ( , 2012 demonstrated that OVGP1 and heparin-like glycosaminoglycans from the oviductal fluid of sows and cows participate in the functional modification of the ZP, affecting the spermoocyte interaction and contributing to the control of polyspermy. Besides, OVGP1 and sperm interactions increased rates of fertilization and embryonic development (Killian, 2004).…”

Section: Oviductal Fluidmentioning

confidence: 99%

“…Besides, OVGP1 and sperm interactions increased rates of fertilization and embryonic development (Killian, 2004). In addition, it is suggested that OVGP1 stabilizes the microenvironment surrounding by gametes and embryo, preventing dispersal of essential nutrients and ions, particularly during ciliary beating or muscular contraction, increasing the viscosity of luminal fluid (Hunter, 1994;Mondejar et al, 2012).…”

Section: Oviductal Fluidmentioning

confidence: 99%

Role of the oviduct and oviduct-derived products in ruminant embryo development

Rizos¹,

Maillo²,

Lonergan³

2016

Anim Reprod

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The fact that embryos can be obtained in vitro undermines the role of the oviduct. However, it has been demonstrated that when in vitro produced bovine zygotes are cultured in the oviduct of sheep, cattle or mice the embryo quality is improved compared to the embryos produced in vitro. Thus the oviduct is not simply a passive organ required only for transporting the embryo to the uterus but also provides a suitable microenvironment for the early embryo. The study of physiological mechanisms and interactions between the embryo and the oviductal environment is essential to understand the correct processes of early embryo developmental. This knowledge can be used to improve current in vitro procedures providing high quality embryos capable of continued development and implantation, and resulting in viable births.

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“…[6][7][8][9][10][11] However, their role in the fertilization process was not partially clarified until recently when it has been showed that plasminogen is present in the oviductal fluid and that plasminogen, tPA, and uPA are immunolocalized on the ZP and oolemma of mature bovine and porcine oocytes. 4 These authors showed that after fertilization, plasminogen, tPA, and uPA immunolabeling decreased in oocytes, suggesting the activation of plasminogen into plasmin. Moreover, it has been shown that addition of both plasminogen and plasmin in the IVF medium decreased the sperm penetration suggesting that plasmin is responsible for the observed effect.…”

Section: Introductionmentioning

confidence: 98%

“…1 The fertilization process may include some steps requiring protein degradation such as sperm penetration through the zona pellucida (ZP), 2 the remodeling of the ZP, the regulation of the viscosity of the surrounding perivitelline fluid around the oocyte membrane, 3 or others. Then, the plasminogen/plasmin system is a candidate to play a role in fertilization, especially in the light of recent studies demonstrating that plasminogen is activated into plasmin during gamete interaction 4 and the generated plasmin reduces the number of sperm penetrating the oocyte during in vitro fertilization (IVF). 5 However, it is still unknown how this reduction occurs.…”

Section: Introductionmentioning

confidence: 99%

How Is Plasminogen/Plasmin System Contributing to Regulate Sperm Entry Into the Oocyte?

et al. 2013

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Plasminogen is present in the oviduct, on the zona pellucida (ZP) and on oolemma, and reduces the number of sperm penetrating the oocyte during in vitro fertilization in pig and cow. It is unknown how this reduction occurs. We tested whether plasminogen (1) changed the ZP resistance to enzymatic digestion thus making the passage of the spermatozoa across it difficult; (2) reduced the sperm functionality, assessed by sperm viability, motility, spontaneous acrosome reaction and membrane lipid disorder; or (3) affected the sperm-ZP binding before or after sperm-ZP interaction. The mechanism by which plasminogen/plasmin system contributes to regulate sperm entry into the oocyte is not inducing a ZP hardening or a decrease in sperm functionality but detaching more than 50% of sperm bound to the ZP. It is suggested that the fertilizing spermatozoon activates plasminogen into plasmin at the oocyte surface and that plasmin removes additional spermatozoa attached to the ZP.

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Fertilization outcome could be regulated by binding of oviductal plasminogen to oocytes and by releasing of plasminogen activators during interplay between gametes

Cited by 34 publications

References 50 publications

Oviductal secretion and gamete interaction

Oviductal secretion and gamete interaction

Role of the oviduct and oviduct-derived products in ruminant embryo development

How Is Plasminogen/Plasmin System Contributing to Regulate Sperm Entry Into the Oocyte?

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