Ferulago angulata as a Good Radioprotector Against Genotoxicity

Abstract

Introduction: Natural products can be used as radioprotector agents because of containing phenolic compounds and several flavonoids with antioxidant properties. When the normal cells are exposed to ionizing radiation, they generate free radicals and reactive oxygen species that can cause damage in DNA, which leads to cellular dysfunction or even cell death. However, it is necessary to identify new radioprotective agents to protect normal cells. Ferulago angulata (F.angulata), a medicinal plant, can be used as a new radioprotective agent. Purpose: The antioxidant activity of F.angulata was assayed using FRAP and DPPH methods. Then, the human blood samples were incubated with F.angulata at different concentrations (25, 50, 100 and 200 μM) and subsequently exposed to IR at a dose of 2Gy. The radioprotective effectof F.angulata on the exposed cells was assessed by micronucleus (MN) method. Also, biomarkers of oxidative stress in the exposed cells were evaluated by malondialdehyde (MDA) and superoxide dismutase (SOD) methods. Methods: The antioxidant activity of F.angulata was assayed using FRAP and DPPH methods. Then, the human blood samples were incubated with F.angulata at different concentrations (25, 50, 100 and 200 μM) and subsequently exposed to IR at a dose of 2Gy. The radioprotective effectof F.angulata on the exposed cells was assessed by micronucleus (MN) method. Also, biomarkers of oxidative stress in the exposed cells were evaluated by malondialdehyde (MDA) and superoxide dismutase (SOD) methods. Results: Our findings showed that F. angulata reduced the frequency of MN induced by IR in exposed cells. At 200μM concentration of F. angulata, maximum reduction in the frequency of MN (63.11%) was observed that demonstrated a high degree of radioprotection. Afterward, pretreatment at 200μM concentration of F.angulata inhibited oxidative stress in irradiated lymphocytes, leading to a reduction in MN frequency and MDA levels while SOD activity was enhanced in the exposed cells. Conclusion: F. angulata as a natural radioprotective agent can protect normal cells against reactive oxygen species and genetic damage induced by IR.

Radioprotective effect of a novel and green bio‐nanohybrid, chitosan/silver/cobalt complex, based on Ferulago angulate plant

“…Previous studies indicate Ferulago angulate decreases MDA levels in serum through increasing antioxidant capacity 26 . Amifostine is used for radioprotection in human as the only FDA‐approved drug, but side effects (such as hypotension, nausea, and vomiting) restricted its use in patients during exposure to IR.…”

“…First, 50—200 μM of bio‐nanocomposite and 70 μl of FRAP reagent were incubated at 37°C for 5 min and absorbance was observed. The FRAP values and standard curve were shown in micromoles per liter (μM) and millimole Fe 2+ , respectively 24 …”

Radioprotective Effect of Zinc Nanoparticles on Ionizing Radiation-induced Nephrotoxicity in Mice

“…Treatment with Zn increased CAT and SOD activity and decreased MDA level in rats suffering from kidney oxidative stress induced by cadmium [50]. The electromagnetic radiation-induced oxidative stress via decreasing GSH and increasing MDA in kidney and plasma of rats, and the treatment with zinc mitigated the oxidative stress [15,17,51,52]. Treatment with another antioxidant agent such as carnitine in irradiated animals reduced the oxidative stress potential of radiation in lung and liver as vital organs and improved their function in comparison to the untreated group [46].…”

“…Kidney is an IR sensitive organ because the function and morphology of kidney as well as phenotype of kidney cells are affected by IR [12]. Several investigations have reported the protective effects of antioxidants scavenging free radicals as radio protectors [13][14][15][16][17].…”

Review of Phyto-Medical Extracts’ and Compounds’ Anti-Radiation Properties

“…Moshafi et al assessed the radioprotective effects of F. angulata on human blood lymphocytes. Their findings demonstrated that the 200 μg plant protected the cells from oxidative damage and gene toxicity, as demonstrated by a decrease in MDA and micronuclear frequency and an increase in superoxidase enzyme levels[106]. Similarly, Seyedi et al demonstrated that a bionanocomposites of Chitosan-Co-Silver complex synthesized from F. angulata as a reducing agent, decreased MN frequency of human lymphocytes exposed to ionization radiation, possibly due to its antioxidant activity[107].…”