Feruloyl oligosaccharides, isolated from bacterial fermented wheat bran, exhibit antioxidant effects in IPEC‐J2 cells and zebrafish model

Abstract

Feruloyl oligosaccharides (FOs) were produced by solid‐state fermentation of wheat bran using Bacillus subtilis, Bacillus licheniformis, and Saccharomyces cerevisiae, and its antioxidant activity was investigated using IPEC‐J2 cells and zebrafish embryo model. Preliminary structure analysis revealed that FOs has an average molecular weight of 11.81 kDa and consists of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose, and fucose. The obtained FOs possess superior reducing power and DPPH and hydroxyl free radical scavenging activities. In IPEC‐J2 cells, antioxidant enzymes activities and GSH level were significantly increased, while MDA level was reduced by FOs. Further studies showed that FOs achieved the aforementioned effects by activating Nrf2 signaling pathway. In zebrafish embryo, FOs effectively suppressed ROS production, lipid peroxidation, and cell death by increasing SOD and GSH‐Px activities. Our findings suggested that FOs from solid‐state fermented wheat bran with mixed bacteria can be used as an antioxidant food additive or drugs.

Study on antioxidant activity of wheat bran extract microcapsules in vitro and in vivo

“…Chen et al . (2022) found that FOs showed superior antioxidant activity in a zebrafish embryonic model. Under the condition of low dose, this paper was consistent with previous research results and showed that WBE had a good antioxidant function in vivo , and had a synergistic antioxidant effect with β‐carotene.…”

“…Study has shown that the strong radical scavenging activity of FOs in WBE is closely related to the FA and xylose components of their structure (Chen et al ., 2022). Zhang et al .…”

“…(2019) was adopted to prepare crude wheat bran extracts. Then, the crude WBE solution was purified by a macroporous resin column, 50% ethanol eluant (Chen et al ., 2022) was collected and WBE was obtained by freeze‐drying.…”

“…The main component of WBE is ferulic oligosaccharide (FOs) which is composed of xylan and ferulic acid (Chen et al ., 2022). Ferulic acid, one of the most abundant phenolic compounds in wheat bran, is a natural phenolic acid.…”

Antioxidant properties of wheat bran FOs prepared by Bacillus amyloliquefaciensIT‐45 fermentation

“…Xyl and Ara were the main monosaccharides of wheat bran FOs. In total, ten types of monosaccharides in purified wheat bran FOs through co-culture fermentation were identified by Chen et al, and Xyl and Ara were the main types of monosaccharide with molar ratios of 33.42% and 29.81%, respectively (Chen et al, 2023). Zhao et al explored the antioxidant properties of different polymerisation degrees of wheat bran FOs.…”