Fetal bovine serum: geographic origin and regulatory relevance of viral contamination

Hawkes, Percy

doi:10.1186/s40643-015-0063-7

Cited by 41 publications

(30 citation statements)

References 3 publications

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“…New Zealand only imports BSA from Australia [ 38 ] and is an exporter of BSA to other countries. These restrictions were implemented to prevent importation of bovine spongiform encephalopathy (BSE) prions [ 39 ], but do not address the risks of the importation of BVDV, and remains an area for both countries to address.…”

Section: A New Zealand and Australian Perspectivementioning

confidence: 99%

Perspectives on Current Challenges and Opportunities for Bovine Viral Diarrhoea Virus Eradication in Australia and New Zealand

2018

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This review outlines the history of bovine viral diarrhoea virus (BVDV) and the current situation in Australia and New Zealand. BVDV has been reported as present in cattle from both countries for close to 60 years. It rates as the second most economically significant disease afflicting cattle, and is highly prevalent and spread throughout the beef and dairy industries. While other cattle diseases have been the subject of government control and eradication, infection with BVDV is presently not. Eradication has been undertaken in many other countries and been judged to be a good investment, resulting in positive economic returns. Presently, Australia and New Zealand have adopted a non-compulsory approach to control schemes, initiated and managed by farmers and veterinarians without the ultimate goal of eradication. Moving towards eradication is possible with the infrastructure both countries possess, but will require additional resources, coordination, and funding from stakeholders to move to full eradication.

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Section: A New Zealand and Australian Perspectivementioning

confidence: 99%

Perspectives on Current Challenges and Opportunities for Bovine Viral Diarrhoea Virus Eradication in Australia and New Zealand

2018

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“…CHO cells are permissive to multiple types of viruses such as Adenovirus, Parainfluenza virus, Bovine respiratory syncytial virus, Vesicular stomatitis virus, Reovirus, and Bluetongue virus [14]. All the viruses listed above could be transmitted from either HEK-293 cells [11,12,13] or fetal bovine serum [8]. We believe there are unknown or undetected viruses existing in HEK-293 and bovine serum.…”

Section: Resultsmentioning

confidence: 99%

“…Serum is a potential source of contamination by viruses, mycoplasmas, and even prions. It has been reported that Bovine respiratory syncytial virus, Bovine viral diarrhea virus, Rabies virus, Vesicular stomatitis virus, Bluetongue virus, and Foot and mouth disease virus could be isolated from bovine serum [8]. To avoid contamination by potential infectious viruses from serum, retrovectors could be packaged in suspension HEK-293 cells cultured in a serumfree or CD medium.…”

Section: Introductionmentioning

confidence: 99%

Retrovectors packaged in CHO cells to generate GLP-1-Fc stable expression CHO cell lines

Suzhen²,

Cao³

et al. 2019

Electronic Journal of Biotechnology

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Background: Chinese hamster ovary (CHO) cells are the most dependable mammalian cells for the production of recombinant proteins. Replication-incompetent retroviral vector (retrovector) is an efficient tool to generate stable cell lines. Multiple copies of integrated genes by retrovector transduction results in improved recombinant protein yield. HEK-293 and their genetic derivatives are principal cells for retrovector production. Retrovectors packaged in HEK-293 cells pose a risk of infectious agent transmission, such as viruses and mycoplasmas, from serum and packaging cells. Results: In this report, retrovectors were packaged in CHO cells cultured in chemically defined (CD) media. The retrovectors were then used to transduce CHO cells. This method can block potential transmission of infectious agents from serum and packaging cells. With this method, we generated glucagon-like protein-1 Fc fusion protein (GLP-1-Fc) stable expression CHO cell lines. Productivity of GLP-1-Fc can reach 3.15 g/L. The GLP-1-Fc protein produced by this method has comparable bioactivity to that of dulaglutide (Trulicity). These stable cell lines retain 95-100% of productivity after 40 days of continuous culture (~48-56 generations). Conclusions: Suspension CHO cells are clean, safe, and reliable cells for retrovector packaging. Retrovectors packaged from this system could be used to generate CHO stable cell lines for recombinant protein expression. How to cite: Li J, Wei S, Cao C, et al. Retrovectors packaged in CHO cells to generate GLP-1-Fc stable expression CHO cell lines. Electron J Biotechnol 2019;41. https://doi.

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“…In addition to scientific disadvantages such as unknown exact composition, seasonal and geographical lot-to-lot variability (Baker, 2016b), and unintended interaction with test substances (Groothuis et al, 2015;Kramer et al, 2009), which can lead to unexpected or undesired outcomes, serious safety concerns for laboratory personnel in terms of endotoxins, mycoplasma and viral contaminants or prion proteins (Dormont, 1999;Wessman and Levings, 1999;Hawkes, 2015), as well as ethical concerns vine fetuses in the last third of their development, if it takes place on EU territory, is thus a procedure under the Directive because the serum is clearly used for scientific purposes and is extracted from a living animal. Therefore, the procedures would need to undergo a project review and authorization by a competent authority.…”

Section: Problems Of Fbs Usementioning

confidence: 99%

Fetal bovine serum (FBS): Past – present – future

Valk

Bieback

Buta³

et al. 2018

ALTEX

276

222

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Fetal bovine serum: geographic origin and regulatory relevance of viral contamination

Abstract: The article aims to clarify an existing misunderstanding by the users of fetal bovine serum (FBS)

Cited by 41 publications

References 3 publications

Perspectives on Current Challenges and Opportunities for Bovine Viral Diarrhoea Virus Eradication in Australia and New Zealand

Perspectives on Current Challenges and Opportunities for Bovine Viral Diarrhoea Virus Eradication in Australia and New Zealand

Retrovectors packaged in CHO cells to generate GLP-1-Fc stable expression CHO cell lines

Fetal bovine serum (FBS): Past – present – future

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