Fetal bovine serum xenoproteins modulate human monocyte adhesion and protein release on biomaterials in vitro

Schmidt, David; Joyce, Evan J.; Kao, Weiyuan John

doi:10.1016/j.actbio.2010.08.022

Cited by 19 publications

(13 citation statements)

References 45 publications

(56 reference statements)

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“…Several groups already demonstrated that differences in serum concentration or type could highly affect cell adhesion (Fujiwara et al, 2009;Grinnell and Feld, 1979;Schmidt et al, 2011). Our study confi rmed that serum greatly affects cell adhesion in addition to surface patterning.…”

Section: Wwwecmjournalorg E Lamers Et Al Dynamic Cell Adhesion Andsupporting

confidence: 59%

Dynamic cell adhesion and migration on nanoscale grooved substrates

Lamers

Riet²,

Domański³

et al. 2012

eCM

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Organised nanotopography mimicking the natural extracellular matrix can be used to control morphology, cell motility, and differentiation. However, it is still unknown how specifi c cell types react with specifi c patterns. Both initial adhesion and preferential cell migration may be important to initiate and increase cell locomotion and coverage with cells, and thus achieve an enhanced wound healing response around an implantable material. Therefore, the aim of this study was to evaluate how MC3T3-E1 osteoblast initial adhesion and directional migration are infl uenced by nanogrooves with pitches ranging from 150 nm up to 1000 nm. In this study, we used a multipatterned substrate with fi ve different groove patterns and a smooth area with either a concentric or radial orientation. Initial cell adhesion measurements after 10 s were performed using atomic force spectroscopy-assisted single-cell force spectroscopy, and demonstrated that nascent cell adhesion was highly induced by a 600 nm pitch and reduced by a 150 nm pitch. Addition of RGD peptide signifi cantly reduced adhesion, indicating that integrins and cell adhesive proteins (e.g. fi bronectin or vitronectin) are key factors in specifi c cell adhesion on nanogrooved substrates. Also, cell migration was highly dependent on the groove pitch; the highest directional migration parallel to the grooves was observed on a 600 nm pitch, whereas a 150 nm pitch restrained directional cell migration. From this study, we conclude that grooves with a pitch of 600 nm may be favourable to enhance fast wound closure, thereby promoting tissue regeneration.

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Section: Wwwecmjournalorg E Lamers Et Al Dynamic Cell Adhesion Andsupporting

confidence: 59%

Dynamic cell adhesion and migration on nanoscale grooved substrates

Lamers

Riet²,

Domański³

et al. 2012

eCM

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“…The serum was added in a minimum dose for stimulation of cell adhesion to plastic [30]. In the monolayer passage 1 RPE culture, 90-95% cells had epithelial phenotype (Fig.…”

Section: Cultures Of Passage 1 and Passage 3 Rpe Cells Grownmentioning

confidence: 99%

3D-Technology of the Formation and Maintenance of Single Dormant Microspheres from 2000 Human Somatic Cells and Their Reactivation In Vitro

et al. 2014

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We developed an original reproducible 3D-technology for preparation of single dormant microspheres consisting of 2000 somatic cells. The dynamics of microsphere assembly from mesenchymal and epithelial cells of retinal pigment epithelium was traced using time-lapse microscopy: formation of a loose aggregate over 24 h followed by its gradual consolidation and formation of a compact viable microsphere with a diameter of 100-150 μ by day 7. The cell number in the formed microspheres remains unchanged. Reactivation observed upon fusion of epithelial and/or mesenchymal microspheres results in the formation of a united compact microtissue. The fusion dynamics reproduces spherogenesis irrespective of the initial amount of co-cultured microspheres. Reactivation via two-step induced angiogenesis opens new prospects for production of vascularized microspheres and microtissues.

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“…For example, differences were observed in adherent density and cytokine release profiles of primary human monocytes exposed to TCPS and poly(ethylene glycol) (PEG) hydrogel surfaces cultured in either fetal bovine serum (FBS) or autologous human serum (AHS) [12]. The effect of serum source was most evident in the observed 3- and 10-fold increase in adherent cell density on TCPS and PEG hydrogel surfaces, respectively, when AHS versus FBS was employed.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, this decrease in adherent density is usually accompanied by a temporal alteration in cell behavior ( e.g. cytokine or growth factor release) [11, 12, 21–24]. The mechanism regulating the decrease in monocyte adherent density on these substrates and its possible impact on cell behavior, however, remains unclear.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, we explored active monocyte detachment via MMPs or calpains and cellular death as explanations for decreased monocytes density in vitro over time on TCPS and PEG hydrogel surfaces. TCPS was selected because it is a commonly used reference material and PEG hydrogels were selected because they have been shown to limit protein adsorption while supporting monocyte adhesion while having differing properties than TCPS [12, 13]. Additionally, extracellular TNF-α and ROS production were analyzed for a potential causal relationship with monocyte or PMN death.…”

Section: Introductionmentioning

confidence: 99%

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Active leukocyte detachment and apoptosis/necrosis on PEG hydrogels and the implication in the host inflammatory response

et al. 2012

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Monocytes/Macrophages have long been recognized as key players in inflammation and wound healing and are often employed in vitro to gain an understanding of the inflammatory response to biomaterials. Previous work has demonstrated a drastic decrease in primary monocyte adherent density on biomaterial surfaces coupled with a change in monocyte behavior over time. However, the mechanism responsible for this decrease was unclear. In this study, we explored active detachment and cellular death as possible regulating factors. Specifically, extracellular TNF-α and ROS production were analyzed as potential endogenous stimulators of cell death. MMPs, but not calpains, were found to play a key role in active monocyte detachment. Monocyte death was found to peak at 24hr and occur by both apoptosis and necrosis as opposed to polymorphonuclear leukocyte death which mainly occurred through apoptosis. Finally, TNF-α and ROS production were not found to have a causal relationship with monocyte death on TCPS or PEG surfaces. The occurrence of primary monocyte apoptosis/necrosis as well as active detachment from a material surface has implications not only in in vitro study, but also in the translation of the in vitro inflammatory response of these cells to in vivo applications.

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Fetal bovine serum xenoproteins modulate human monocyte adhesion and protein release on biomaterials in vitro

Cited by 19 publications

References 45 publications

Dynamic cell adhesion and migration on nanoscale grooved substrates

Dynamic cell adhesion and migration on nanoscale grooved substrates

3D-Technology of the Formation and Maintenance of Single Dormant Microspheres from 2000 Human Somatic Cells and Their Reactivation In Vitro

Active leukocyte detachment and apoptosis/necrosis on PEG hydrogels and the implication in the host inflammatory response

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