Fetal exposures and perinatal influences on the stool microbiota of premature infants

Chernikova, Diana A.; Koestler, Devin C.; Hoen, Anne G.; Housman, Molly L.; Hibberd, Patricia L.; Moore, Jason H.; Morrison, Hilary G.; Sogin, Mitchell L.; Zain-Ul-Abideen, Muhammad; Madan, Juliette C.

doi:10.3109/14767058.2014.987748

Cited by 46 publications

(62 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding is similar to those of previous studies focused on the use of maternal antibiotics in general, particularly with the decrease in Bacteroides and Bifidobacterium. [38][39][40][41][42][43]46,47 One study 40 observed significant effects of GBS prophylaxis on the infant gut microbiome at 6 months among 150 pregnancies for members of Clostridiaceae, Ruminococcoceae and Enterococcaceae whereas another 41 (n = 83) found lower levels of Bifidobacterium and Escherichia at 12 weeks of life in infants of mothers exposed to intrapartum antibiotics.…”

Section: Main Findingsmentioning

confidence: 99%

Specific class of intrapartum antibiotics relates to maturation of the infant gut microbiota: a prospective cohort study

Coker

Hoen

Dade

et al. 2019

BJOG

100

View full text Add to dashboard Cite

Objective To evaluate the potential impact of intrapartum antibiotics, and their specific classes, on the infant gut microbiota in the first year of life. Design Prospective study of infants in the New Hampshire Birth Cohort Study (NHBCS). Settings Rural New Hampshire, USA. Population or sample Two hundred and sixty-six full-term infants from the NHBCS. Methods Intrapartum antibiotic use during labour and delivery was abstracted from medical records. Faecal samples collected at 6 weeks and 1 year of age were characterised by 16S rRNA sequencing, and metagenomics analysis in a subset of samples. Exposures Maternal exposure to antibiotics during labour and delivery. Main outcome measure Taxonomic and functional profiles of faecal samples. Results Infant exposure to intrapartum antibiotics, particularly to two or more antibiotic classes, was independently associated with lower microbial diversity scores as well as a unique bacterial community at 6 weeks (GUnifrac, P = 0.02). At 1 year, infants in the penicillin-only group had significantly lower α diversity scores than infants not exposed to intrapartum antibiotics. Within the first year of life, intrapartum exposure to penicillins was related to a significantly lower increase in several taxa including Bacteroides, use of cephalosporins was associated with a significantly lower rise over time in Bifidobacterium and infants in the multi-class group experienced a significantly higher increase in Veillonella dispar. Conclusions Our findings suggest that intrapartum antibiotics alter the developmental trajectory of the infant gut microbiome, and specific antibiotic types may impact community composition, diversity and keystone immune training taxa.

show abstract

Section: Main Findingsmentioning

confidence: 99%

Specific class of intrapartum antibiotics relates to maturation of the infant gut microbiota: a prospective cohort study

Coker

Hoen

Dade

et al. 2019

BJOG

100

View full text Add to dashboard Cite

show abstract

“…Such fetuses are thus exposed to sources of potentially virulent microbes. Exposure to these insults leads to a less diverse infant gut microbiome, with relatively higher numbers of pathogenic bacteria being present (Chernikova et al, ). Infections during pregnancy are well known to interrupt normal gestation by both altering the vaginal flora as in bacterial vaginosis, or by increasing susceptibilities for other infections that could alter both maternal and infant microbiomes (Redelinghuys et al, ).…”

Section: Part Imentioning

confidence: 99%

The very low birth weight infant microbiome and childhood health

Groër

Gregory

Louis‐Jacques

et al. 2015

Birth Defects Research Pt C

View full text Add to dashboard Cite

This review describes current understandings about the nature of the very low birth weight infant (VLBW) gut microbiome. VLBW infants often experience disruptive pregnancies and births, and prenatal factors can influence the maturity of the gut and immune system, and disturb microbial balance and succession. Many VLBWs experience rapid vaginal or Caesarean births. After birth these infants often have delays in enteral feeding, and many receive little or no mother's own milk. Furthermore the stressors of neonatal life in the hospital environment, common use of antibiotics, invasive procedures and maternal separation can contribute to dysbiosis. These infants experience gastrointestinal dysfunction, sepsis, transfusions, necrotizing enterocolitis, oxygen toxicity, and other pathophysiological conditions that affect the normal microbiota. The skin is susceptible to dysbiosis, due to its fragility and contact with NICU organisms. Dysbiosis in early life may resolve but little is known about the timing of the development of the signature gut microbiome in VLBWs. Dysbiosis has been associated with a number of physical and behavioral problems, including autism spectrum disorders, allergy and asthma, gastrointestinal disease, obesity, depression, and anxiety. Dysbiosis may be prevented or ameliorated in part by prenatal care, breast milk feeding, skin to skin contact, use of antibiotics only when necessary, and vigilance during infancy and early childhood.

show abstract

“…In recent years, the long-held theory that the womb is sterile has been met with an increasing body of evidence to the contrary. Numerous 16S rDNA next-generation sequencing (NGS) studies have reported on a fetal gut microbiome (Mshvildadze et al 2010;Gosalbes et al 2013;Hu et al 2013;Moles et al 2013;Ardissone et al 2014;Del Chierico et al 2015;Hansen et al 2015;Chernikova et al 2016;Collado et al 2016;Wampach et al 2017). However, while these studies have detected bacterial DNA in the fetal gut, they have not been able to provide information on whether this DNA comes from viable organisms.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the bacterial microbiome in first‐pass meconium using propidium monoazide ( PMA ) to exclude nonviable bacterial DNA

Stinson

Keelan

Payne

2019

Lett Appl Microbiol

View full text Add to dashboard Cite

Numerous studies have reported bacterial DNA in first‐pass meconium samples, suggesting that the human gut microbiome is seeded prior to birth. However, these studies have not been able to discriminate between DNA from living bacterial cells, DNA from dead bacterial cells or cell‐free DNA. Here we have used propidium monoazide (PMA) together with 16S rRNA gene sequencing to determine whether there are intact bacterial cells in the fetal gut. DNA was extracted from first‐pass meconium (n = 5) and subjected to 16S rRNA gene sequencing with/without PMA treatment. All meconium samples, regardless of PMA treatment, contained detectable levels of bacterial DNA; however, treatment with PMA prior to DNA extraction decreased the DNA yield by approximately 20%. PMA‐treated meconium samples did not differ significantly from untreated samples in terms of observed number of OTUs (P = 0·945); although they did differ taxonomically, with around one quarter of OTUs identified in untreated samples only, suggesting that they have originated from cell‐free/nonviable DNA. The mean Sørensen coefficient for treated vs untreated samples was 0·527. Our findings suggest that the fetal gut is seeded with intact bacterial cells prior to birth. This is an important finding, as exposure to live bacteria during gestation might have a significant impact on the developing fetus. Significance and Impact of the Study DNA‐based microbiome studies performed using 16S rRNA gene sequencing are limited by their inability to discriminate between live bacterial cells, dead bacterial cells and cell‐free DNA. Here we use propidium monoazide (PMA) to exclude nonviable bacteria from microbiome analysis of first‐pass meconium samples and thereby reveal that the majority of the purported fetal gut microbiome is from intact bacterial cells. This work demonstrates the importance of excluding nonviable bacteria when analysing the microbial community in low‐biomass samples such as meconium.

show abstract

Fetal exposures and perinatal influences on the stool microbiota of premature infants

Cited by 46 publications

References 30 publications

Specific class of intrapartum antibiotics relates to maturation of the infant gut microbiota: a prospective cohort study

Specific class of intrapartum antibiotics relates to maturation of the infant gut microbiota: a prospective cohort study

The very low birth weight infant microbiome and childhood health

Characterization of the bacterial microbiome in first‐pass meconium using propidium monoazide ( PMA ) to exclude nonviable bacterial DNA

Contact Info

Product

Resources

About