Fetal Tissue Engineering from Amniotic Fluid

Kaviani, Amir; Guleserian, Kristine J.; Perry, Tjörvi E.; Jennings, Russell W.; Ziegler, Moritz M.; Fauza, Dario O.

doi:10.1016/s1072-7515(02)01834-3

Cited by 116 publications

(85 citation statements)

References 25 publications

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“…Fetal-associated tissues such as placenta, amniotic liquid, and umbilical cord are potential sources of cells for tissue engineering. [42][43][44][45] Human fe-BONE CELL GROWTH IN PLA COMPOSITE SCAFFOLDS tal liver cells have been used for transplantation when there was no perfectly matched donor for marrow transplantation. 46 Neuronal conditions such as Huntington's 47 or Parkinson's disease 48 have been treated with fetal neuroblasts.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of Bioresorbable Poly(L-lactic acid) Composite Scaffolds Obtained by Supercritical Gas Foaming with Human Fetal Bone Cells

et al. 2005

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The aim of this investigation was to test the biocompatibility of three-dimensional bioresorbable foams made of poly(L-lactic acid) (PLA), alone or filled with hydroxyapatite (HA) or ␤-tricalcium phosphate (␤-TCP), with human primary osteoblasts, using a direct contact method. Porous constructs were processed by supercritical gas foaming, after a melt-extrusion of ceramic/polymer mixture. Three neat polymer foams, with pore sizes of 170, 310, and 600 m, and two composite foams, PLA/5 wt% HA and PLA/5 wt% ␤-TCP, were examined over a 4-week culture period. The targeted application is the bone tissue-engineering field. For this purpose, human fetal and adult bone cells were chosen because of their highly osteogenic potential. The association of fetal bone cells and composite scaffold should lead to in vitro bone formation. The polymer and composite foams supported adhesion and intense proliferation of seeded cells, as revealed by scanning electron microscopy. Cell differentiation toward osteoblasts was demonstrated by alkaline phosphatase (ALP) enzymatic activity, ␥-carboxylated Gla-osteocalcin production, and the onset of mineralization. The addition of HA or ␤-TCP resulted in higher ALP enzymatic activity for fetal bone cells and a stronger production of Gla-osteocalcin for adult bone cells.

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Section: Discussionmentioning

confidence: 99%

Biocompatibility of Bioresorbable Poly(L-lactic acid) Composite Scaffolds Obtained by Supercritical Gas Foaming with Human Fetal Bone Cells

et al. 2005

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“…Amniotic epithelial cells obtained from cesarean sections have been shown to express markers for neuronal, glial and progenitor cells and to differentiate into neuron-like cells in the ischemic brain of adult rats [7][8][9]. Moreover, there is evidence that amniotic fluid contains fetal mesenchymal stem cells with a multilineage differentiation potential [10][11][12][13]. In this study we extensively characterized unselected amniotic cells and tested their multilineage differentiation capacity in vitro.…”

Section: Introductionmentioning

confidence: 99%

Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential

et al. 2006

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“…Fetal-associated tissues such as placenta, amniotic liquid, or umbilical cord are described to be potential source of cells for tissue engineering [17][18][19][20]. In contrast to embryonic derived up to the end of the eighth week, beginning at the ninth week, cells are considered as fetal and tissue dissection as organ donation.…”

Section: Introductionmentioning

confidence: 99%

Fetal bone cells for tissue engineering

Montjovent

Burri

Mark

et al. 2004

Bone

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We envision the use of human fetal bone cells for engineered regeneration of adult skeletal tissue. A description of their cellular function is then necessary. To our knowledge, there is no description of human primary fetal bone cells treated with differentiation factors. The characterization of fetal bone cells is particularly important as the pattern of secreted proteins from osteoblasts has been shown to change during aging. In the first part of this work, human primary fetal bone cells were compared to adult bone cells and mesenchymal stem cells for their ability to proliferate and to differentiate into osteoblasts in vitro. Cell proliferation, gene expression of bone markers, alkaline phosphatase (ALP) activity, and mineralization were analyzed during a time-course study. In the second part of this paper, bone fetal cells behavior exposed to osteogenic factors is further detailed. The doubling time of fetal bone cells was comparable to mesenchymal stem cells but significantly shorter than for adult bone cells. Gene expression of cbfa-1, ALP, a1 chain of type I collagen, and osteocalcin were upregulated in fetal bone cells after 12 days of treatment, with higher inductions than for adult and mesenchymal stem cells. The increase of ALP enzymatic activity was stronger for fetal than for adult bone cells reaching a maximum at day 10, but lower than for mesenchymal stem cells. Importantly, the mineralization process of bone fetal cells started earlier than adult bone and mesenchymal stem cells. Proliferation of fetal and adult bone cells was increased by dexamethasone, whereas 1a,25-dihydroxyvitamin D 3 did not show any proliferative effect. Mineralization studies clearly demonstrated the presence of calcium deposits in the extracellular matrix of fetal bone cells. Nodule formation and calcification were strongly increased by the differentiation treatment, especially by dexamethasone. This study shows for the first time that human primary fetal bone cells could be of great interest for bone research, due to their fast growth rate and their ability to differentiate into mature osteoblasts. They represent an interesting and promising potential for therapeutic use in bone tissue engineering.

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Fetal Tissue Engineering from Amniotic Fluid

Cited by 116 publications

References 25 publications

Biocompatibility of Bioresorbable Poly(L-lactic acid) Composite Scaffolds Obtained by Supercritical Gas Foaming with Human Fetal Bone Cells

Biocompatibility of Bioresorbable Poly(L-lactic acid) Composite Scaffolds Obtained by Supercritical Gas Foaming with Human Fetal Bone Cells

Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential

Fetal bone cells for tissue engineering

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