FGF-2 Enhances Vascularization for Adipose Tissue Engineering

Marra, Kacey G.; Defail, Alicia J.; Clavijo-Alvarez, Julio A.; Badylak, Stephen F.; Taieb, Aurele; Schipper, Bret M.; Bennett, Jennifer M.; Rubin, J Peter

doi:10.1097/01.prs.0000305517.93747.72

Cited by 68 publications

(51 citation statements)

References 34 publications

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“…24 Synthetic microsphere encapsulation has also been used to trap bFGF in PLGA, incorporating these microspheres into alginate scaffolds or simply injecting them with small intestinal submucosa and preadipocytes, both of which have been shown to significantly enhance vascularization. 25,26 In terms of biodegradable microspheres, chitosan-gelatin microspheres containing bFGF have been developed and incorporated into a porous chitosan-gelatin scaffold, demonstrating initial fast release kinetics over 2 days before achieving a steady release profile lasting 2 weeks, displaying promise for vascularization. 27 Ultimately, the ability to direct the timing and release of growth factors will control the degree of vascularization within the tissue.…”

Section: Growth Factor Deliverymentioning

confidence: 99%

Vascularization Strategies for Tissue Engineering

Lovett

Lee

Edwards

et al. 2009

Tissue Engineering Part B: Reviews

813

685

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Tissue engineering is currently limited by the inability to adequately vascularize tissues in vitro or in vivo. Issues of nutrient perfusion and mass transport limitations, especially oxygen diffusion, restrict construct development to smaller than clinically relevant dimensions and limit the ability for in vivo integration. There is much interest in the field as researchers have undertaken a variety of approaches to vascularization, including material functionalization, scaffold design, microfabrication, bioreactor development, endothelial cell seeding, modular assembly, and in vivo systems. Efforts to model and measure oxygen diffusion and consumption within these engineered tissues have sought to quantitatively assess and improve these design strategies. This review assesses the current state of the field by outlining the prevailing approaches taken toward producing vascularized tissues and highlighting their strengths and weaknesses.

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Section: Growth Factor Deliverymentioning

confidence: 99%

Vascularization Strategies for Tissue Engineering

Lovett

Lee

Edwards

et al. 2009

Tissue Engineering Part B: Reviews

813

685

View full text Add to dashboard Cite

show abstract

“…Bioactive peptide molecules derived from mammalian extracellular matrix (ECM) have been shown to have potent chemoattractive and mitogenic effects upon endogenous progenitor and stem cells (13)(14)(15)(16). Although full characterization of these bioactive peptides has not been completed to date, ex vivo enzymatic, chemical, and physical methods (17)(18)(19), and in vivo physiologic methods (20), have generated a heterogeneous population of ECM peptides (13,21) with both chemotactic and mitogenic properties for a variety of stem and progenitor cells.…”

mentioning

confidence: 99%

Epimorphic regeneration approach to tissue replacement in adult mammals

Agrawal

Johnson²,

Reing³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

143

134

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Urodeles and fetal mammals are capable of impressive epimorphic regeneration in a variety of tissues, whereas the typical default response to injury in adult mammals consists of inflammation and scar tissue formation. One component of epimorphic regeneration is the recruitment of resident progenitor and stem cells to a site of injury. Bioactive molecules resulting from degradation of extracellular matrix (ECM) have been shown to recruit a variety of progenitor and stem cells in vitro in adult mammals. The ability to recruit multipotential cells to the site of injury by in vivo administration of chemotactic ECM degradation products in a mammalian model of digit amputation was investigated in the present study. Adult, 6- to 8-week-old C57/BL6 mice were subjected to midsecond phalanx amputation of the third digit of the right hind foot and either treated with chemotactic ECM degradation products or left untreated. At 14 days after amputation, mice treated with ECM degradation products showed an accumulation of heterogeneous cells that expressed markers of multipotency, including Sox2, Sca1, and Rex1 (Zfp42). Cells isolated from the site of amputation were capable of differentiation along neuroectodermal and mesodermal lineages, whereas cells isolated from control mice were capable of differentiation along only mesodermal lineages. The present findings demonstrate the recruitment of endogenous stem cells to a site of injury, and/or their generation/proliferation therein, in response to ECM degradation products.

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“…Fibroblast growth factor-2 (FGF-2) and fibroblast growth factor-10 (FGF-10) are also potent inducers of angiogenesis secreted by adipocytes [13,14,26]. Hepatocyte growth factor (HGF) is also secreted by adipocytes and stimulates angiogenesis [3,39].…”

mentioning

confidence: 99%

Fat Depot-Specific Differences in Angiogenic Growth Factor Gene Expression and Its Relation to Adipocyte Size in Cattle

Yamada

Kawakami

Nakanishi

2010

The Journal of Veterinary Medical Science

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ABSTRACT. Adipocytes derived from different anatomical sites vary in the expression of adipocytokines and growth factor genes. Adipogenesis is tightly associated with angiogenesis, although the regional variation of angiogenic growth factor gene expression in adipose tissues remains unclear. In this experiment, we studied the fat depot-specific differences (subcutaneous, intramuscular, intermuscular, renal, and mesenteric) in the expression of angiogenic growth factor mRNA [vascular endothelial growth factor (VEGF), fibroblast growth factor-2 (FGF-2), fibroblast growth factor-10 (FGF-10), hepatocyte growth factor (HGF), and leptin], as well as the relationship between angiogenic growth factor mRNA level and adipocyte size in bovine adipose tissues. Intermuscular, renal, and mesenteric adipose tissues expressed significantly higher VEGF, FGF-2, and leptin mRNA levels than did subcutaneous and intramuscular adipose tissues. Mesenteric adipose tissue also expressed higher FGF-10 mRNA levels than did subcutaneous and intramuscular adipose tissues. There was no significant difference in the expression of HGF mRNA among adipose tissue depots. A significant correlation existed between adipocyte size and VEGF, FGF-2, FGF-10, and leptin mRNA levels. These results indicate that fat depot-specific difference in angiogenic growth factor gene expression results from the difference in adipocyte size.

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FGF-2 Enhances Vascularization for Adipose Tissue Engineering

Abstract: Small intestinal submucosa particles are a favorable scaffold for preadipocytes, allowing ex vivo proliferation on particles small enough to be injected. Delivery of FGF-2 from poly(lactic-co-glycolic acid) microspheres resulted in cell survival and enhanced vascularization.

Cited by 68 publications

References 34 publications

Vascularization Strategies for Tissue Engineering

Vascularization Strategies for Tissue Engineering

Epimorphic regeneration approach to tissue replacement in adult mammals

Fat Depot-Specific Differences in Angiogenic Growth Factor Gene Expression and Its Relation to Adipocyte Size in Cattle

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