FGF adsorbed mesoporous bioactive glass with larger pores in enhancing bone tissue engineering

Wang, Xiaoyan; Liu, Qianqian; Chen, Wei; Liu, Long

doi:10.1007/s10856-019-6252-8

Cited by 12 publications

(5 citation statements)

References 30 publications

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“…Many in vivo experiments have verified that new bone is produced in relatively large amounts when used in fresh tooth extraction wounds or different types of bone defects [16,49,50]. When incorporated with different growth factors, the osteogenic power is significantly enhanced [51,52]. The bone substitute selected in this study was an innovative product developed based on primary 45S5 bioactive glass.…”

Section: Discussionmentioning

confidence: 99%

Scaffolds of bioactive glass (Bioglass®) combined with recombinant human bone morphogenetic protein -9 (rhBMP-9) for tooth extraction site preservation

Shi

Zhou

Jingbo

et al. 2022

Heliyon

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The study aimed to investigate the osteogenic ability of bioactive glass (bioglass) combined with recombinant human bone morphogenetic protein-9 (rhBMP-9) on rat bone marrow mesenchymal stem cells (BMSCs) in vitro. The study also compares bone regeneration using rhBMP9 soaked with different carrier systems, including bioglass or collagen membranes (BioGide, BG) in a rat alveolar bone site preservation model in vivo. Methods: Scanning electron microscopy was employed to analyze bioglass surface. The absorption and release potential of rhBMP9 from bioglass were researched by ELISA. The cell viability, adhesion, proliferation, and differentiation were assessed for rhBMP9 soaked on bioglass by cck-8 kit, alkaline phosphatase (ALP) activity assay, alizarin red staining, and real-time PCR. Furthermore, prepared grafts (bioglass þ BG, bioglass/rhBMP9þBG, and bioglass þ BG/rhBMP9) were implanted into the maxillary right first incisor sockets of Sprague Dawley rats for 8 weeks, and new bone formation was quantified by micro-CT and histological analysis. Results: Bioglass absorbed rhBMP9 dramatically and released it with a slow and stable speed within ten days by ELISA. When used with cck-8 kit detection, cell viability at 24 h, cell adhesion rate at 8 h, and cell proliferation at 1, 3, and 5 days were decreased in the bioglass alone group versus the control group but slightly increased with the addition of rhBMP9. Similarly, the effect of osteogenic differentiation on bioglass increased significantly when combined with rhBMP9 by upregulating the expression of ALP, mineralized matrix, and osteogenic related genes. Furthermore, both bioglass/rhBMP9þBG samples and bioglass þ BG/rhBMP9 samples significantly improved several bone formation parameters compared with bioglass þ BG samples. Interestingly, bioglass þ BG/rhBMP9 samples demonstrated more bone regeneration in rat site preservation models. Conclusions: Both bioglass and BG can be applied in GBR surgery as effective carriers of rhBMP9. However, BG may be more suitable than bioglass for investigating site preservation effect after tooth extraction when associated with rhBMP9 and provides a practical clinical solution to the problem of bone deficiency caused by alveolar bone atrophy.

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Section: Discussionmentioning

confidence: 99%

Scaffolds of bioactive glass (Bioglass®) combined with recombinant human bone morphogenetic protein -9 (rhBMP-9) for tooth extraction site preservation

Shi

Zhou

Jingbo

et al. 2022

Heliyon

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“…The three groups of cells were together incubated for different days, with five individual wells for every group corresponding to specific culture days. The proliferation was measured under adsorbance at 450 nm by adding a Cell Counting Kit-8 (CCK-8, Beyotime Biotech, Haimen, China) to the cell medium, followed by 4 h of incubation at 37 °C [ 70 ].…”

Section: Methodsmentioning

confidence: 99%

“…RT-qPCR was reacted with cDNA through SYBR Green (Applied Biosystems, Waltham, MA, USA). The forward and reverse primers were used as follows: bone morphogenetic protein 2 ( Bmp2 ) 5′-CTGACCACCTGAACTCCAC-3′ and 5′-CATCTAGGTACAACATGGAG-3′; osteopontin ( Opn ) 5′-TCCAAAGCCAGCCTGGAAC-3′ and 5′-TGACCTCAGAAGATGAACTC-3′; Osterix 5′-GTCAAGAGTCTTAGCCAAACTC-3′ and 5′-AAATGATGTGAGGCCAGATGG-3′; runt-related transcription factor 2 ( Runx2 ) 5′-GAATGCACTACCCAGCCAC-3′ and 5′-TGGCAGGTACGTGTGGTAG-3′; glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ) 5′-CATGGCCTTCCGTGTTCCTA-3′ and 5′-CCTGCTTCACCACCTTCTTGAT-3′ [ 70 ]; angiotensin ( ANG ) 5′-GTGCTGGGTCTGGGTCTGAC-3′ and 5′-GGCCTTGATGCTGCGCTTG-3′; platelet endothelial cell adhesion molecule-1 ( CD31 ) 5′-CAACGAGAAAATGTCAGA-3′ and 5′-GGAGCCTTCCGTTCTAGAGT-3′; hypoxia-inducible factor-1α ( HIF-1a ) 5′-CCAGATCTCGGCGAAGTAAAG-3′ and 5′-GCTGATGGTAAGCCTCATCAC-3′; Von Willebrand factor ( vWF ) 5′-CCCCTGAAGCCCCTCCTCCTA-3′ and 5′-ACGAACGCCACATCCAGAACC-3′; Colony-Stimulating Factor 1 Receptor ( Csf1r ) 5′-CCTGCGATGTGTGAGCAATG-3′ and 5′-CGGATAATGAACCCTCGCCA-3′; Gremlin 1 ( Grem1 ) 5′-GAATCGCACCGCATACACTG-3′ and 5′-TGGCTCCTTGGGAACCTTTC-3′; Collagen Triple Helix Repeat Containing Protein 1 ( Cthrc1 ) 5′-GGTCGGGATGGATTCAAAGG-3′ and 5′-AGCGAACTCCACGAACACTG-3′; and Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain 2 ( Cited2 ) 5′-CTCATGGGCGAGCACATACA-3′ and 5′-GAGTTGTTAAACCTGGCGGC-3′. The whole process of RT-qPCR included the following steps: first 95 °C for 10 min, 56 °C for 60 s, and 72 °C for 30 s; followed 44 cycles of 95 °C for 30 s, 56 °C for 60 s, and 72 °C for 30 s; finally, 4 °C to stop.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Angiogenesis-Based Scaffold of MesoporousBioactive Glass Nanofiber on Osteogenesis

Zheng

Bai

Huang

et al. 2022

IJMS

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There is still an urgent need for more efficient biological scaffolds to promote the healing of bone defects. Vessels can accelerate bone growth and regeneration by transporting nutrients, which is an excellent method to jointly increase osteogenesis and angiogenesis in bone regeneration. Therefore, we aimed to prepare a composite scaffold that could promote osteogenesis with angiogenesis to enhance bone defect repair. Here, we report that scaffolds were prepared by coaxial electrospinning with mesoporous bioactive glass modified with amino (MBG-NH2) adsorbing insulin-like growth factor-1 (IGF-1) as the core and silk fibroin (SF) adsorbing vascular endothelial growth factor (VEGF) as the shell. These scaffolds were named MBG-NH2/IGF@SF/VEGF and might be used as repair materials to promote bone defect repair. Interestingly, we found that the MBG-NH2/IGF@SF/VEGF scaffolds had nano-scale morphology and high porosity, as well as enough mechanical strength to support the tissue. Moreover, MBG-NH2 could sustain the release of IGF-1 to achieve long-term repair. Additionally, the MBG-NH2/IGF@SF/VEGF scaffolds could significantly promote the mRNA expression levels of osteogenic marker genes and the protein expression levels of Bmp2 and Runx2 in bone marrow mesenchymal stem cells (BMSCs). Meanwhile, the MBG-NH2/IGF@SF/VEGF scaffolds promoted osteogenesis by simulating Runx2 transcription activity through the phosphorylated Erk1/2-activated pathway. Intriguingly, the MBG-NH2/IGF@SF/VEGF scaffolds could also significantly promote the mRNA expression level of angiogenesis marker genes and the protein expression level of CD31. Furthermore, RNA sequencing verified that the MBG-NH2/IGF@SF/VEGF scaffolds had excellent performance in promoting bone defect repair and angiogenesis. Consistent with these observations, we found that the MBG-NH2/IGF@SF/VEGF scaffolds demonstrated a good repair effect on a critical skull defect in mice in vivo, which not only promoted the formation of blood vessels in the haversian canal but also accelerated the bone repair process. We concluded that these MBG-NH2/IGF@SF/VEGF scaffolds could promote bone defect repair under accelerating angiogenesis. Our finding provides a new potential biomaterial for bone tissue engineering.

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“…In some applications, micrometric pore size can present a great advantage when the functionalization of the BaG needs to be realized with larger molecular weight substances [ 73 ]. The porosity of the material has been proven to be influential in processes such as remineralization [ 74 ].…”

Section: Bioactive Glassmentioning

confidence: 99%

Bone Regeneration and Oxidative Stress: An Updated Overview

et al. 2022

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Bone tissue engineering is a complex domain that requires further investigation and benefits from data obtained over past decades. The models are increasing in complexity as they reveal new data from co-culturing and microfluidics applications. The in vitro models now focus on the 3D medium co-culturing of osteoblasts, osteoclasts, and osteocytes utilizing collagen for separation; this type of research allows for controlled medium and in-depth data analysis. Oxidative stress takes a toll on the domain, being beneficial as well as destructive. Reactive oxygen species (ROS) are molecules that influence the differentiation of osteoclasts, but over time their increasing presence can affect patients and aid the appearance of diseases such as osteoporosis. Oxidative stress can be limited by using antioxidants such as vitamin K and N‑acetyl cysteine (NAC). Scaffolds and biocompatible coatings such as hydroxyapatite and bioactive glass are required to isolate the implant, protect the zone from the metallic, ionic exchange, and enhance the bone regeneration by mimicking the composition and structure of the body, thus enhancing cell proliferation. The materials can be further functionalized with growth factors that create a better response and higher chances of success for clinical use. This review highlights the vast majority of newly obtained information regarding bone tissue engineering, such as new co-culturing models, implant coatings, scaffolds, biomolecules, and the techniques utilized to obtain them.

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FGF adsorbed mesoporous bioactive glass with larger pores in enhancing bone tissue engineering

Cited by 12 publications

References 30 publications

Scaffolds of bioactive glass (Bioglass®) combined with recombinant human bone morphogenetic protein -9 (rhBMP-9) for tooth extraction site preservation

Scaffolds of bioactive glass (Bioglass®) combined with recombinant human bone morphogenetic protein -9 (rhBMP-9) for tooth extraction site preservation

The Effect of Angiogenesis-Based Scaffold of MesoporousBioactive Glass Nanofiber on Osteogenesis

Bone Regeneration and Oxidative Stress: An Updated Overview

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