FGF signaling and target recognition in the developing xenopus visual system

McFarlane, Sarah; McNeill, Lisa; Holt, Christine E.

doi:10.1016/0896-6273(95)90091-8

Cited by 168 publications

(135 citation statements)

References 79 publications

Supporting

Mentioning

130

Contrasting

Unclassified

Order By: Relevance

“…Embryos were then bathed either in a control solution containing DMSO, which was used to dilute the SB-3CT, or different concentrations (10-75 µM) of SB-3CT. These were slightly higher than those used previously in an in vitro study (Aye et al, 2004), but routinely higher concentrations of drug are required in the in vivo exposed brain preparation (McFarlane et al, 1995;Webber et al, 2002).…”

Section: Resultsmentioning

confidence: 85%

Matrix metalloproteinases are required for retinal ganglion cell axon guidance at select decision points

2005

Self Cite

View full text Add to dashboard Cite

Axons receive guidance information from extrinsic cues in their environment in order to reach their targets. In the frog Xenopus laevis, retinal ganglion cell (RGC) axons make three key guidance decisions en route through the brain. First, they cross to the contralateral side of the brain at the optic chiasm. Second, they turn caudally in the mid-diencephalon. Finally,they must recognize the optic tectum as their target. The matrix metalloproteinase (MMP) and a disintegrin and metalloproteinase (ADAM)families are zinc (Zn)-dependent proteolytic enzymes. The latter functions in axon guidance, but a similar role has not yet been identified for the MMP family. Our previous work implicated metalloproteinases in the guidance decisions made by Xenopus RGC axons. To test specifically the importance of MMPs, we used two different in vivo exposed brain preparations in which RGC axons were exposed to an MMP-specific pharmacological inhibitor(SB-3CT), either as they reached the optic chiasm or as they extended through the diencephalon en route to the optic tectum. Interestingly, SB-3CT affected only two of the guidance decisions, with misrouting defects at the optic chiasm and tectum. Only at higher concentrations was RGC axon extension also impaired. These data implicate MMPs in the guidance of vertebrate axons, and suggest that different metalloproteinases function to regulate axon behaviour at distinct choice points: an MMP is important in guidance at the optic chiasm and the target, while either a different MMP or an ADAM is required for axons to make the turn in the mid-diencephalon.

show abstract

Section: Resultsmentioning

confidence: 85%

Matrix metalloproteinases are required for retinal ganglion cell axon guidance at select decision points

2005

Self Cite

View full text Add to dashboard Cite

show abstract

“…These growth factors have been further characterized by a recurrent set of events in a wound-healing context: injury-induced growth factor production and release, growth factor-induced proliferative and migratory re- sponses and morphologic changes in target cells, additional growth factor regulation through feedback loops and a specific termination process (25). Endogenous basic fibroblast growth factor (bFGF) levels, a member of the FGF family (26), have been shown to increase at injury sites (27).…”

Section: Discussionmentioning

confidence: 99%

Caveolin-1 as a Novel Indicator of Wound-Healing Capacity in Aged Human Corneal Epithelium

Rhim

Kim

Yeo

et al. 2010

Mol Med

View full text Add to dashboard Cite

Excess caveolin-1 has been reported to play a role in age-dependent hyporesponsiveness to growth factors in vitro. Therefore, we hypothesized that caveolin-1-dependent hyporesponsiveness to growth factors in aged corneal epithelial cells might be responsible for delayed wound healing in vivo. To test this hypothesis, we evaluated corneal wound-healing time by vital staining using fluorescein after laser epithelial keratomileusis (LASEK). We compared wound-healing times in young, middle-aged and elderly patients. We also examined caveolin-1 levels and other aging markers, such as p53 and p21, in the corneal epithelium. Elderly patients generally had higher caveolin-1 levels in the corneal epithelia than young patients. There were, however, variations among individuals with increased caveolin-1 in some young patients and decreased levels in some elderly patients. Wound-healing time after LASEK correlated well with the corneal caveolin-1 status. Therefore, we suggest that caveolin-1 status might be responsible for delayed wound healing in elderly patients after LASEK. Caveolin-1 status might be a regulator for wound-healing capacity and a novel target for in vivo adjustment.

show abstract

“…Although slits could be the ADAM10 target in the mid-diencephalon, they are unlikely to be the molecular substrate at the optic tectum because no defects were observed in target innervation in the mouse mutants. One possible cleavage substrate at the tectum is suggested by the similarity of the target recognition phenotype seen with ADAM10 inhibition and disruption of fibroblast growth factor signaling (McFarlane et al, 1995(McFarlane et al, , 1996Walz et al, 1997). Alternatively, two recent studies suggest that the secreted Tlr (Tolloid-related) metalloproteinase functions in the recognition of muscle targets by Drosophila motor axons by promoting defasciculation of axons from the motor nerve in the vicinity of the target (Meyer and Aberle, 2006;Serpe and O'Connor, 2006).…”

Section: Discussionmentioning

confidence: 99%

Targeting of Retinal Axons Requires the Metalloproteinase ADAM10

Chen

Hehr²,

Atkinson‐Leadbeater³

et al. 2007

J. Neurosci.

View full text Add to dashboard Cite

The role of extrinsic cues in guiding developing axons is well established; however, the means by which the activity of these extrinsic cues is regulated is poorly understood. A disintegrin and metalloproteinase (ADAM) enzymes are Zn-dependent proteinases that can cleave guidance cues or their receptors in vitro. Here, we identify the first example of a metalloproteinase that functions in vertebrate axon guidance in vivo. Specifically, ADAM10 is required for formation of the optic projection by Xenopus retinal ganglion cell (RGC) axons. Xadam10 mRNA is expressed in the dorsal neuroepithelium through which RGC axons extend. Pharmacological or molecular inhibition of ADAM10 within the brain each resulted in a failure of RGC axons to recognize their target. In contrast, molecular inhibition of ADAM10 within the RGC axons themselves had no effect. These data argue strongly that in the dorsal brain ADAM10 acts cell non-autonomously to regulate the guidance of RGC axons.

show abstract

FGF signaling and target recognition in the developing xenopus visual system

Cited by 168 publications

References 79 publications

Matrix metalloproteinases are required for retinal ganglion cell axon guidance at select decision points

Matrix metalloproteinases are required for retinal ganglion cell axon guidance at select decision points

Caveolin-1 as a Novel Indicator of Wound-Healing Capacity in Aged Human Corneal Epithelium

Targeting of Retinal Axons Requires the Metalloproteinase ADAM10

Contact Info

Product

Resources

About