FGF10: Type III Epithelial Mesenchymal Transition and Invasion in Breast Cancer Cell Lines

Abstract

Purpose: Fibroblastic growth factor-10 (FGF-10) has an important role in type I epithelial mesenchymal transition (EMT) during the embryonic period of life (gastrulation). Since EMT has a critical role during cancer cells invasion and metastasis (type III) this study sought to investigate the possible role of FGF-10 in type III EMT by monitoring breast cancer cell lines' behavior by FGF-10 regulation.Methods: MCF-7 and MDA-MB-231 cell lines with different levels of FGF10 expression were treated with FGF-10 recombinant protein and FGF-10 siRNA, respectively.Results: The cell viability, migration, colony formation and wound healing have a direct relationship with FGF-10 expression, while FGF-10 expression decreased apoptosis. All mesenchymal factors (such as vimentin, N cadherin, snail, slug, TGF-β) increased due to FGF-10 expression with contrary expression of epithelial markers (such as E-cadherin). Moreover, GSK3β phosphorylation (inactivation) increased with FGF-10 expression.Conclusion: The important role of FGF-10 in type III EMT on cancer cells and initiation of metastasis via various kinds of signaling pathways has been suggested.

Fibroblast growth factor-10 and epithelial-mesenchymal transition in colorectal cancer

“…Our previous study showed that FGF-10 is effective in EMT induction through the alteration of epithelial and mesenchymal markers in breast cancer cell lines. This confirms the significance of this EMT inducer in EMT type 3 (Abolhassani et al, 2014[1]). …”

“…EMT type 1 happens during the early stages of life and is related to gastrulation and development (Shirkoohi, 2013[37]). The important role of FGF-10 in EMT type 1 has previously been reported (Abolhassani et al, 2014[1]), being vital for the appropriate development of stomach, duodenum and cecum in mice (Sala et al, 2006[33]). EMT type 2 is related to tissue regeneration and organ fibrosis, while EMT type 3 is pivotal during cancer progression and metastasis (Kalluri and Weinberg, 2009[20]).…”

“…This growth factor also has a role in epithelial-mesenchymal transition (EMT), wound healing and differentiation of embryonic stem cells (Itoh, 2016[19]). Furthermore, a correlation between the invasion of cancer cells and FGF-10 gene expression levels in some cancers such as prostate cancer has been reported (Abolhassani et al, 2014[1]).…”

Expression of Fibroblast Growth Factor 10 Is Correlated with Poor Prognosis in Gastric Adenocarcinoma

“…As a specific ligand of FGFR2 and one of FGF family, FGF10 had been proved to be associated with the progression and prognosis in many kinds of cancers, including breast cancer, lung cancer, and bladder cancer (Chung and Koh 2013;Abolhassani et al 2014;Suzuki et al 2015). Moreover, the oncogenic role of FGF10 paracrine signaling pathway has been revealed (Itoh and Ohta 2014).…”

“…FGF10 knockdown siRNA and scrambled siRNA were purchased from Santa Cruz Company (sc-39462) according to previous report (Abolhassani et al 2014). Transfection of siRNA into gastric cancer cells was performed with reagent RNAiMAX (Invitrogen Company, USA) according to the manual.…”