FgTep1p is linked to the phosphatidylinositol‐3 kinase signalling pathway and plays a role in the virulence of <i>Fusarium graminearum</i> on wheat

Zhang, Dajun; Feng, Fan; Yang, Jingran; Wang, Xiuli; Qiu, Dewen; Jiang, Lijing

doi:10.1111/j.1364-3703.2010.00626.x

Cited by 13 publications

(16 citation statements)

References 43 publications

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“…The study indicates that ptn1 plays a role in virulence and teliospore germination. This is consistent with the observations from S. cerevisiae (Heymont et al, 2000) and F. graminearum (D. Zhang et al, 2010), where the mutant strains showed defect in sporulation.…”

Section: Chapter V Discussionsupporting

confidence: 92%

“…Moreover, FgTep1p (PTEN orthologue) deletion mutants in the wheat pathogen, Fusarium graminearum, produced fewer conidia and showed an attenuation in virulence on host plants (D. Zhang et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Researchers became interested in understanding the role of this conserved pathway in other eukaryotic systems. Studies have been conducted in C. elegans (Ogg & Ruvkun, 1998), D. rerio (Stumpf, Choorapoikayil, & den Hertog, 2015), D. melanogaster (Huang et al, 1999a), E. coioides (Luo et al, 2016), S. cerevisiae (Heymont et al, 2000), and F. graminearum (D. Zhang et al, 2010). The presence of an orthologue of the PTEN gene in Ustilago maydis prompted us to study the role of this gene in this fungal system.…”

Section: Chapter V Discussionmentioning

confidence: 99%

“…A study conducted in F. graminearum focusing on its pathogenesis indicated the presence of a PTEN orthologue, FgTEP1. FgTEP1 of F. graminearum is the functional homologue of TEP1 gene of S. cerevisiae (D. Zhang et al, 2010). The functional similarity of FgTEP1 to that of yeast TEP1 was demonstrated mainly through the wortmannin resistant phenotype and a specific phenotype that is connected to treatment with lithium ions.…”

Section: Saccharomyces Cerevisiae the Budding Yeast Saccharomyces Cmentioning

confidence: 99%

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Exploring the role of PTN1 in ustilago maydis.

Pillai¹,

Krishna²

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Signal transduction is a key aspect of biological life. PTEN (phosphatase and tensin homolog) is a widely studied signaling protein in mammalian systems, where it acts as a tumor suppressor. Numerous studies have conclusively demonstrated the anti-cancerous effect of this protein. Mutations in the PTEN gene have been implicated in several disorders including cancer, diabetes, and Cowden syndrome, among others. It is part of the PI3K-PTEN-AKT-mTOR signaling pathway and is responsible for various metabolic and cellular activities. This pathway is found to be conserved across several eukaryotic systems including yeast, Drosophila melanogaster and Caenorhabditis elegans. While PTEN orthologues have been characterized in ascomycete fungi, it has not been reported in any basidiomycete fungi. The initial part of the current study was aimed at the characterization of PTEN orthologue, ptn1, of Ustilago maydis, a basidiomycete fungus. When the U. maydis, ptn1 gene was either deleted or overexpressed, it was found that the deletion of this gene lead to reduced virulence, spore production, and germination rate; effects of the overexpression were more subtle or not discernable. Previous studies have indicated that two other signaling proteins, Rho1 and Pdc1, are physical interactors of Ptn1. The goal of the latter part of the present study was to explore the functional interaction of Ptn1 with vi Rho1 and Pdc1. Our experiments indicated a functional connection between these genes, especially in processes that are related to stress tolerance, cellular morphology, and virulence of the fungus, suggesting that they may be elements of the same regulatory pathway. We predict that the pdc1 interaction with ptn1 may be the reverse of that of rho1 with ptn1. In addition, our experiments with an orthologue of S6K1, aga1, showed that its deletion leads to some of the same phenotypes seen with that of ptn1, pdc1 and/or rho1. The current phenotypes of these mutant strains when considered in the context of already available information about the role of these genes in other systems indicate that they may be part of a pathway that modulates cytoskeletal rearrangements. vii

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Section: Chapter V Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Chapter V Discussionmentioning

confidence: 99%

Section: Saccharomyces Cerevisiae the Budding Yeast Saccharomyces Cmentioning

confidence: 99%

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Exploring the role of PTN1 in ustilago maydis.

Pillai¹,

Krishna²

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“…Deletion of the PTEN orthologue in Schizosaccharomyces pombe resulted in the accumulation of its substrate PI [3,4,5]P3, as well as misshapen vacuoles and sensitivity to osmotic stress, while in the budding yeast Saccharomyces cerevisiae it caused mislocalisation of dityrosine during sporulation and increased resistance to the phosphoinositide-3 kinase inhibitor, wortmannin (Heymont et al, 2000;Mitra et al, 2004). In Fusarium graminearum, mutants lacking Fgtep1 displayed reduced conidiation, decreased germination in the presence of wortmannin, and reduced virulence on wheat coleoptiles (Zhang et al, 2010). Similarly, in the corn pathogen Ustilago maydis, deletion of Umptn1 caused a decrease in teliospore production, germination and virulence (Vijayakrishnapillai et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

A homologue of the mammalian tumour suppressor protein PTEN is a functional lipid phosphatase and required for chemotaxis in filamentous fungi

Hassing

Candy

Eaton

et al. 2020

Preprint

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SummaryPhosphoinositides (PI) are essential components of eukaryotic membranes and function in a large number of signalling processes. While lipid second messengers are well studied in mammals and yeast, their role in filamentous fungi is poorly understood. We used fluorescent PI-binding molecular probes to localise the phosphorylated phosphatidylinositol species PI[3]P, PI[3,5]P2, PI[4]P and PI[4,5]P2 in hyphae of the endophyte Epichloë festucae in axenic culture and during interaction with its grass host Lolium perenne. We also analysed the roles of the phosphatidylinositol-4-phosphate 5-kinase MssD and the predicted phosphatidylinositol-3,4,5-triphosphate 3-phosphatase TepA, a homologue of the mammalian tumour suppressor protein PTEN. Deletion of tepA in E. festucae and in the root-infecting tomato pathogen Fusarium oxysporum had no impact on growth in culture or the host interaction phenotype. However, this mutation did uncover the presence of PI[3,4,5]P3 in septa of E. festucae and showed that TepA is required for chemotropism in F. oxysporum. The identification of PI[3,4,5]P3 in septa of ΔtepA strains suggests that filamentous fungi are able to generate PI[3,4,5]P3 using an alternative biosynthetic pathway and that fungal PTEN homologues are functional lipid phosphatases. The F. oxysporum chemotropism defect demonstrates a conserved role of PTEN homologues in chemotaxis across protists, fungi and mammals.

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Process of Infection by Fungal Pathogens

2017

Microbial Plant Pathogens

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FgTep1p is linked to the phosphatidylinositol‐3 kinase signalling pathway and plays a role in the virulence of Fusarium graminearum on wheat

Cited by 13 publications

References 43 publications

Exploring the role of PTN1 in ustilago maydis.

Exploring the role of PTN1 in ustilago maydis.

A homologue of the mammalian tumour suppressor protein PTEN is a functional lipid phosphatase and required for chemotaxis in filamentous fungi

Process of Infection by Fungal Pathogens

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