FH535 Inhibited Migration and Growth of Breast Cancer Cells

Iida, Joji; Dorchak, Jesse; Lehman, J.; Clancy, R. L.; Luo, Chunxiong; Chen, Yaqin; Somiari, Stella; Ellsworth, Rachel E.; Hu, Hai; Mural, Richard J.; Shriver, Craig D.

doi:10.1371/journal.pone.0044418

Cited by 28 publications

(20 citation statements)

References 54 publications

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“…Supplemented with FH535, the protein abundance of β‐catenin was suppressed, which was in accordance with Polk (), Iida et al . () and Liu et al . ().…”

Section: Discussionmentioning

confidence: 90%

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Effect of FH535 on in vitro maturation of porcine oocytes by inhibiting WNT signaling pathway

Shi

Cheng

et al. 2017

Animal Science Journal

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Wingless-int (WNT) signaling pathway is vital to modulate life processes, including cell fate determination, cell differentiation, cell proliferation, cell apoptosis and embryogenesis. To demonstrate the uncertain effect of the canonical WNT signaling pathway on oocyte maturation, immature porcine oocytes were collected and cultured in vitro with the WNT/β-catenin inhibitor FH535. The concentrations of FH535 were selected as 0.00, 0.01, 0.10, 1.00 and 10.00 μmol/L. The results showed that the optimum concentration of FH535 on oocyte maturation was 1.00 μmol/L. In this concentration, the proportion of MII oocytes increased (P < 0.05). The rate of cleavage was the same with the control (P > 0.05), while the rate of blastocysts in the 1.00 μmol/L FH535 treated group was higher than that of control (P < 0.01). Additionally, the average number of nuclei in blastocysts raised significantly (P < 0.05). The inhibition of WNT could regulate expression of maturation-related genes, including Cdc-2, Bmp-15, Gdf-9 and Mos. In the 1.00 μmol/L FH535 treated group, the messenger RNA level of β-catenin showed no significant change compared to the control (P > 0.05), but the protein abundance was decreased (P < 0.05). This study revealed that the inhibition of FH535 on the WNT signaling pathway could promote the maturation of porcine oocytes and altered gene expressions in vitro.

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“…Supplemented with FH535, the protein abundance of β‐catenin was suppressed, which was in accordance with Polk (), Iida et al . () and Liu et al . ().…”

Section: Discussionmentioning

confidence: 90%

“…However, there are also some contradictions indicating that FH535 could inhibit the expression of β‐catenin (Iida et al . ; Liu et al . ).…”

Section: Introductionmentioning

confidence: 99%

Effect of FH535 on in vitro maturation of porcine oocytes by inhibiting WNT signaling pathway

Shi

Cheng

et al. 2017

Animal Science Journal

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“…In a previous study [67], H535 at the dose of 15 µmol/L could down-regulate β-catenin expression in HCT-116 and SW48 cells. FH535 at the dose of 0.1 µmol/L also showed to inhibit migration and growth of breast cancer cells [68]. Recent studies have demonstrated that FH535 is a synthetic inhibitor of the canonical WNT-signaling pathway and, not for normal fibroblasts, FH535 also inhibits growth of colon, lung, and hepatocellular carcinoma cell lines [67].…”

Section: Discussionmentioning

confidence: 98%

γ-Tocotrienol Induces Paraptosis-Like Cell Death in Human Colon Carcinoma SW620 Cells

Zhang

et al. 2013

PLoS ONE

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Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

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“…Glycanated variants concentrate in filopodial extensions, including the finest protrusions conventionally denoted "microspikes," where they seem to colocalize with b1, a3, and a4 integrin subunits, ezrin, and neural precursor cell expressed, developmentally downregulated 9 ( Fig. 4 and Table 1) (17,23,(43)(44)(45)(46)(47). However, NG2/CSPG4 molecules lacking GAG chains have also been noted in microclustered configurations confined to putative lipid rafts.…”

Section: Subcellular Organization Of Ng2/cspg4 Glycoforms Is Dictatedmentioning

confidence: 98%

Structural deciphering of the NG2/CSPG4 proteoglycan multifunctionality

et al. 2018

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The chondroitin sulfate proteoglycan 4 (CSPG4) gene encodes a transmembrane proteoglycan (PG) constituting the largest and most structurally complex macromolecule of the human surf aceome. Its transcript shows an extensive evolutionary conservation and, due to the elaborated intracellular processing of the translated protein, it generates an array of glycoforms with the potential to exert variant‐specific functions. CSPG4‐mediated molecular events are articulated through the interaction with more than 40 putative ligands and the concurrent involvement of the ectodomain and cytoplasmic tail. Alternating inside‐out and outside‐in signal transductions may thereby be elicited through a tight functional connection of the PG with the cytoskeleton and its regulators. The potential of CSPG4 to influence both types of signaling mechanisms is also asserted by its lateral mobility along the plasma membrane and its intersection with microdomain‐restricted internalization and endocytic trafficking. Owing to the multitude of molecular interplays that CSPG4 may engage, and thanks to a differential phosphorylation of its intracellular domain accounted by crosstalking signaling pathways, the PG stands out for its unique capability to affect numerous cellular phenomena, including those purporting pathologic conditions. We discuss here the progresses made in advancing our understanding about the structural‐functional bases for the ability of CSPG4 to widely impact on cell behavior, such as to highlight how its multivalency may be exploited to interfere with disease progression.—Tamburini, E., Dallatomasina, A., Quartararo, J., Cortelazzi, B., Mangieri, D., Lazzaretti, M., Perris, R. Structural deciphering of the NG2/CSPG4 proteoglycan multifunctionality. FASEB J. 33, 3112–3128 (2019). http://www.fasebj.org

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FH535 Inhibited Migration and Growth of Breast Cancer Cells

Cited by 28 publications

References 54 publications

Effect of FH535 on in vitro maturation of porcine oocytes by inhibiting WNT signaling pathway

Effect of FH535 on in vitro maturation of porcine oocytes by inhibiting WNT signaling pathway

γ-Tocotrienol Induces Paraptosis-Like Cell Death in Human Colon Carcinoma SW620 Cells

Structural deciphering of the NG2/CSPG4 proteoglycan multifunctionality

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