Fiber-optic sensors based on reagent delivery with controlled-release polymers

Luo, Shufang; Walt, David R.

doi:10.1021/ac00177a019

Cited by 47 publications

(17 citation statements)

References 10 publications

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“…Silanized glass fibers can be treated with acrylamide or 4-aminophenylmercuric acetate (APMA) to generate free amino groups reacting with NHS-biotin for attaining surface biotinylation. 105 Specific interactions have been shown between a SAM of biotinylated thiols and streptavidin, suggesting the possibility of combining a well-ordered SAM structure with the avidin-biotin system. 109 Studies with different SAM showed very low binding of avidin for the close-packed layers and significantly higher binding for the more loosely packed ones.…”

Section: Avidin-biotinmentioning

confidence: 99%

Protein Immobilization Strategies for Protein Biochips

2007

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In the past few years, protein biochips have emerged as promising proteomic and diagnostic tools for obtaining information about protein functions and interactions. Important technological innovations have been made. However, considerable development is still required, especially regarding protein immobilization, in order to fully realize the potential of protein biochips. In fact, protein immobilization is the key to the success of microarray technology. Proteins need to be immobilized onto surfaces with high density in order to allow the usage of small amount of sample solution. Nonspecific protein adsorption needs to be avoided or at least minimized in order to improve detection performances. Moreover, full retention of protein conformation and activity is a challenging task to be accomplished. Although a large number of review papers on protein biochips have been published in recent years, few have focused on protein immobilization technology. In this review, current protein immobilization strategies, including physical, covalent, and bioaffinity immobilization for the fabrication of protein biochips, are described. Particular consideration has been given to oriented immobilization, also referred to as site-specific immobilization, which is believed will improve homogeneous surface covering and accessibility of the active site.

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Section: Avidin-biotinmentioning

confidence: 99%

Protein Immobilization Strategies for Protein Biochips

2007

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“…To solve this problem, continuously sensing reservoir sensors have been developed for reagent renewal, and polymer matrix is commonly used to incorporate the reagent which can be released slowly upon contact with aqueous solution. However, most of such polymeric delivery systems are used for optical sensors [18][19][20][21]. The conventional membrane ISEs have been found to show the fluxes of primary ions from the inner aqueous solution towards the sample solution, which may provide an alternative way for controlled release of analytical reagents.…”

Section: Introductionmentioning

confidence: 99%

Potentiometric measurement of ascorbate by using a solvent polymeric membrane electrode

Guo

Yin

et al. 2008

Talanta

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“…Optical fiber sensors are becoming established analytical tools for remote and in situ sensing due to the improvements provided by optical methods over other sensing techniques, and the widely known and appreciated features associated to fiber optic like electromagnetic immunity, small size, capability of remote measurements and absence (in most cases) of electrical power at the sensing end [1][2][3]. Fiber-optic sensors' characteristics mentioned above are highly suitable, specially in medical sciences, where measurements are required to have a minimum invasive impact.…”

Section: Introductionmentioning

confidence: 99%