Fiber optic SPR biosensing of DNA hybridization and DNA–protein interactions

Pollet, Jeroen; Delport, Filip; Janssen, Kris P. F.; Jans, Karolien; Maes, Guido; Pfeiffer, Helge; Wevers, Martine; Lammertyn, Jeroen

doi:10.1016/j.bios.2009.08.045

Cited by 223 publications

(148 citation statements)

References 25 publications

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“…A detailed description of the FO-SPR biosensor platform can be found in supplementary information and our previous publications (Arghir et al 2015;Pollet et al 2009). …”

Section: Fo-spr Platform and Preparation Of Fiber Probesmentioning

confidence: 99%

“…Standard optical fibers have emerged over the years as excellent alternatives that can compete with the sensitivity and specificity of prism-based SPR sensors, while offering the advantage of low cost and small size greatly desired for developing a POC tool. One such platform, namely fiber optic surface plasmon resonance (FO-SPR), has been previously described by our group as a promising biosensor for protein-and DNA-based assays (Delport et al 2012;Janssen et al 2012;Knez et al 2013;Knez et al 2014;Pollet et al 2011;Pollet et al 2009;Tran et al 2011). The FO-SPR biosensor makes use of multimode optical fibers that facilitate SPR generation and enable implementation of bioassays.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fiber optic-SPR platform for fast and sensitive infliximab detection in serum of inflammatory bowel disease patients

Stappen

Spasić

et al. 2016

Biosensors and Bioelectronics

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“…A detailed description of the FO-SPR biosensor platform can be found in supplementary information and our previous publications (Arghir et al 2015;Pollet et al 2009). …”

Section: Fo-spr Platform and Preparation Of Fiber Probesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fiber optic-SPR platform for fast and sensitive infliximab detection in serum of inflammatory bowel disease patients

Stappen

Spasić

et al. 2016

Biosensors and Bioelectronics

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118

123

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“…The result was also at the same order of association constants with other groups. 11,12 When strong light intensity was incident onto the high-density GNDs, two orders of magnitude enhancement of binding constant was measured.…”

Section: Author(s) All Article Content Except Where Otherwise Notedmentioning

confidence: 99%

Enhancing DNA binding rate using optical trapping of high-density gold nanodisks

et al. 2014

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We present the dynamic study of optical trapping of fluorescent molecules using high-density gold nanodisk arrays. The gold nanodisks were fabricated by electron beam lithography with a diameter of 500 nm and a period of 1 μm. Dark-field illumination showed ∼15 times enhancement of fluorescence near edges of nanodisks. Such enhanced near-field generated an optical trapping force of ∼10 fN under 3.58 × 103 W/m2 illumination intensity as calculated from the Brownian motions of 590 nm polystyrene beads. Kinetic observation of thiolated DNA modified with Cy5 dye showed different binding rates of DNA under different illumination intensity. The binding rate increased from 2.14 × 103 s−1 (I = 0.7 × 103 W/m2) to 1.15 × 105 s−1 (I = 3.58 × 103 W/m2). Both enhanced fluorescence and binding rate indicate that gold nanodisks efficiently improve both detection limit and interaction time for microarrays.

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“…For example, when biomolecules are adsorbed onto the metal surface, it would induce a local refractive index change, and thereby change the SPR extinction and frequency. 9 Complementary base conjugated Au nanoparticle assembly has been applied to detect analyte by visual effects. However, there are scanty studies on recyclable Au nanohybrid sensors.…”

Section: -6mentioning

confidence: 99%

Au/Silica/Fe₂O₃Nanohybrid Preparation and Biosensor Application

2013

Bulletin of the Korean Chemical Society

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Silica-and organic polymer insulated superparamagnetic nanoparticles have been studied and applied within a range of biomedical applications.1,2 Surface insulating layers can decrease the cytotoxicity of the internal magnetic particle core during in vivo-and in vitro applications. Besides the quenching effect on toxicity, surface coating layers can work as platforms to impart additional functionality. Especially, silica surface chemistry serves a good hydrophilic function and augments easy modification for tagging biomedicalfunctioning moieties. 3-6Reliable and rapid monitoring of bio-events and environments can provide early information of surroundings. Realtime sensing systems normally monitor changes in optical or electrical signals, allowing us to quickly recognize variations in our environment.7,8 Surface plasmonic resonance (SPR) is a colligative value of oscillation changes at a metal surface, and its oscillation frequency is much dependent on the refractive index of materials adsorbed onto the metal surface. For example, when biomolecules are adsorbed onto the metal surface, it would induce a local refractive index change, and thereby change the SPR extinction and frequency.9 Complementary base conjugated Au nanoparticle assembly has been applied to detect analyte by visual effects. However, there are scanty studies on recyclable Au nanohybrid sensors. 10In this study, we developed a real-time and recyclable optical biosensor using nanohybrids of Au and superparamagnetic nanoparticles. For utilizing SPR effects, Au nanoparticle was conjugated onto silica coated magnetic nanoparticles (see Figure 1). Controlled silica shell thickness caused tunable SPR effects.First, monodisperse silica-coated Fe 2 O 3 nanoparticles, hereafter noted as SiO 2 /Fe 2 O 3 , were prepared. γ-Fe 2 O 3 nanoparticles were coated with thin (~2 nm) or thick (~17 nm) silica shell employing water-in-cyclohexane reverse microemulsion technique. For a thin (2 nm-thick) silica shell, polyoxyethylene(5)nonylphenyl ether (0.56 mmol) was dispersed in cyclohexane (4.2 mL) by sonication. Then 400 μL of Fe 2 O 3 solution (0.8 mg/mL of cyclohexane) was added. The resulting mixture was vortexed, and ammonium hydroxide (29.4%, 35 μL) was added to form a transparent reverse microemulsion. Next, tetraethylorthosilicate (2 μL, TEOS) was added, and the reaction was continued for 12 h at room temperature. In the case of thick silica shell (17 nmthick) preparation, TEOS content and sol-gel aging time were increased to 20 μL and 48 h, respectively. By adding methanol into the reaction solution, SiO 2 /Fe 2 O 3 nanoparticles were precipitated and collected by a magnet. They were functionalized with amine functionalities, which have been known to have strong affinity with transition metal nanoclusters, such as Fe 3 O 4 , and Au.11,12 In this study, 340 mg of SiO 2 /Fe 2 O 3 were functionalized with 0.075 mmol of n-(2-amino ethyl)-3-aminopropyltrimethoxysilane (AAPS, Gelest) by refluxing in toluene for 30 h. Next, Au was introduced as HAuCl 4 to the surfa...

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Fiber optic SPR biosensing of DNA hybridization and DNA–protein interactions

Cited by 223 publications

References 25 publications

Fiber optic-SPR platform for fast and sensitive infliximab detection in serum of inflammatory bowel disease patients

Fiber optic-SPR platform for fast and sensitive infliximab detection in serum of inflammatory bowel disease patients

Enhancing DNA binding rate using optical trapping of high-density gold nanodisks

Au/Silica/Fe₂O₃Nanohybrid Preparation and Biosensor Application

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Fiber optic SPR biosensing of DNA hybridization and DNA–protein interactions

Cited by 223 publications

References 25 publications

Fiber optic-SPR platform for fast and sensitive infliximab detection in serum of inflammatory bowel disease patients

Fiber optic-SPR platform for fast and sensitive infliximab detection in serum of inflammatory bowel disease patients

Enhancing DNA binding rate using optical trapping of high-density gold nanodisks

Au/Silica/Fe2O3Nanohybrid Preparation and Biosensor Application

Contact Info

Product

Resources

About

Au/Silica/Fe₂O₃Nanohybrid Preparation and Biosensor Application