Today, there is a steadily increasing demand for the application of surfactants as debonding agents in tissue manufacturing. The work presented herein focused on evaluating the debonding ability of cationic alkoxylated amine surfactant on unrefined and refined fibers. The results showed that, as the dosage of the surfactant was increased to 10 mg/g on unrefined pulps, the adsorption of the surfactant on the fibers increased to 5 mg/g, and the tensile and burst indices of the papers made of the modified fibers decreased by as much as 12.6% and 14.9%, respectively. Also, the roughness, strain, and moisture content of the papers were enhanced, whereas the apparent density was reduced. Furthermore, the tear index of the papers increased upon the application of surfactant (10 mg/g) at the expense of reductions in tensile and burst indices, as well as the apparent density at any pressure applied in wet pressing. Also, as the pressure was increased, the surfactant impacted the fiber bonding more significantly. On the other hand, the application of surfactant (10 mg/g) somewhat increased the light scattering coefficient of the papers, regardless of the refining load. Furthermore, the adsorption of the surfactant on refined fibers increased with increasing refining load. However, the influence of the surfactant on the tear, tensile, and burst indices and the apparent density was impaired with increasing refining load. Also, the zero-span tensile index and brightness of the papers varied negligibly upon surfactant application.