Fibre diffraction in the analysis of filamentous virus structure

Kendall, Amy; Stubbs, Gerald

doi:10.1080/0889311x.2015.1025771

Cited by 3 publications

(2 citation statements)

References 121 publications

(158 reference statements)

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“…Isolated virions have been subject of structural investigations from the early experimental stages on up to now, e.g., via X-ray crystallography, electron microscopy, light diffraction, light scattering or flow birefringence analyses [25,62,[92][93][94][95][96][97][98]. They have repeatedly served as model particles for scrutinizing novel technologies, which has, e.g., given rise to a 2.…”

Section: |3|2 Manifold Routes Of Research and Technical Progress Ther...mentioning

confidence: 99%

Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach

Wendlandt,

Britz,

Kleinow

et al. 2024

Preprint

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This article develops a multi-perspective view on motivations and methods for tobamovirus purification through the ages, and presents a novel efficient, easy-to-use approach that can be well-adapted to different species of native and functionalized virions. We survey the various driving forces prompting researchers to enrich tobamoviruses: from the search for the causative agents of mosaic diseases in plants to their increasing recognition as versatile nanocarriers in biomedical and engineering applications. Best practice and also rarely applied options for the serial processing steps required for successful isolation of tobamoviruses are then reviewed. Adaptations for distinct particle species, pitfalls and 'forgotten' or underrepresented technologies are considered as well. The article is topped off with our own development of a method for virion preparation, rooted in historical protocols. It combines selective re-solubilization of polyethylene glycol (PEG) virion raw precipitates with density step gradient centrifugation in biocompatible iodixanol formulations, yielding ready-to-use particle suspensions. This newly established protocol and some considerations for perhaps worthwile further developments could serve as putative stepping stones towards preparation procedures appropriate for routine practical uses of the multivalent soft-matter nanorods.

show abstract

Section: |3|2 Manifold Routes Of Research and Technical Progress Ther...mentioning

confidence: 99%

Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach

Wendlandt,

Britz,

Kleinow

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Isolated virions have been the subject of structural investigations from the early experimental stages, e.g., via X-ray crystallography, EM, light diffraction, light scattering, or flow birefringence analyses [ 25 , 63 , 98 , 99 , 100 , 101 , 102 , 103 , 104 ]. They have repeatedly served as model particles for scrutinizing novel technologies, which has, e.g., given rise to a 2.3 Å-resolved TMV structure upon testing the power of a direct electron detector in cryo-EM single-particle analysis [ 105 ].…”

Section: Introductionmentioning

confidence: 99%

Getting Hold of the Tobamovirus Particle—Why and How? Purification Routes over Time and a New Customizable Approach

Wendlandt,

Britz,

Kleinow

et al. 2024

Viruses

View full text Add to dashboard Cite

This article develops a multi-perspective view on motivations and methods for tobamovirus purification through the ages and presents a novel, efficient, easy-to-use approach that can be well-adapted to different species of native and functionalized virions. We survey the various driving forces prompting researchers to enrich tobamoviruses, from the search for the causative agents of mosaic diseases in plants to their increasing recognition as versatile nanocarriers in biomedical and engineering applications. The best practices and rarely applied options for the serial processing steps required for successful isolation of tobamoviruses are then reviewed. Adaptations for distinct particle species, pitfalls, and ‘forgotten’ or underrepresented technologies are considered as well. The article is topped off with our own development of a method for virion preparation, rooted in historical protocols. It combines selective re-solubilization of polyethylene glycol (PEG) virion raw precipitates with density step gradient centrifugation in biocompatible iodixanol formulations, yielding ready-to-use particle suspensions. This newly established protocol and some considerations for perhaps worthwhile further developments could serve as putative stepping stones towards preparation procedures appropriate for routine practical uses of these multivalent soft-matter nanorods.

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Microstructural metrology of tobacco mosaic virus nanorods during radial compression and heating

2018

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We determined stress-induced deformations and the thermal stability of nanorod-shaped tobacco mosaic virus (TMV) capsids in coffee-ring structures by X-ray nanodiffraction. The hexagonal capsids lattice transforms under compression in the outer boundary zone of the coffee-ring into a tetragonal lattice. The helical pitch of the nanorods increases by about 2.5% across the outer boundary zone while the lateral distance between nanorods decreases continuously across the whole coffee-ring structure by about 2% due to compressive forces. The diffraction patterns show a mixture of helical scattering and Bragg peaks attributed to a lattice of nanorods interlocked by their helical grooves. Thermo-nanodiffraction reveals water loss up to about 100 °C resulting in a reduction of the helical pitch by about 6% with respect to its maximum value and a reduction of the nanorods separation by about 0.5 nm. Up to about 200 °C the pitch is increasing again by about 2%. Secondary crystallization in the bulk reaches a maximum at 150-160 °C. At higher temperatures the crystallinity is continuously decreasing up to about 220 °C. Above about 200 °C and depending on the heating history, the nanorods start disintegrating into small, randomly oriented aggregates.

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Fibre diffraction in the analysis of filamentous virus structure

Cited by 3 publications

References 121 publications

Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach

Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach

Getting Hold of the Tobamovirus Particle—Why and How? Purification Routes over Time and a New Customizable Approach

Microstructural metrology of tobacco mosaic virus nanorods during radial compression and heating

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