Fibre-optic lifetime-based enzyme biosensor

Papkovsky, Dmitri B.; Olah, J.; Kurochkin, Ilya N.

doi:10.1016/0925-4005(93)85298-o

Cited by 22 publications

(10 citation statements)

References 7 publications

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“…Another method for self-referencing consists in the measurement of luminescence decay time. , Since the measurement of decay times in the order of a few nanoseconds (or even picoseconds) so far has required complex and expensive instrumentation (this has changed in recent years, though), labels and indicators were employed with decay times in the order of μs and ms. Decay time-based sensing is widely employed in optical oxygen sensing and in enzyme sensing based on oxygen transduction. − Measurement of decay time also was reported for a fluorescent hydrogen peroxide transducer (a europium(III) complex) for use in a glucose biosensor . A final self-referenced method is based on measurement of fluorescence polarization, − which also is independent of various variables (such as the degree of labeling, photobleaching, quenching, and solvent effects) that make other methods prone to errors.…”

Section: 4 Frequently Used Spectroscopies and Internal Referencingmentioning

confidence: 99%

Optical Biosensors

2008

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Section: 4 Frequently Used Spectroscopies and Internal Referencingmentioning

confidence: 99%

Optical Biosensors

2008

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“…Their mechanisms of detection include molecular spectroscopy using IR absorption [54], UV absorption [55] and Raman scattering [56,57]; measurement of fluorescence intensity [58], quenching [59] and resonance energy transfer [60]; guided-mode spectroscopy using optical fibers [61]; interferometry using resonant mirrors [62] and grating couplers [63,64]; colorimetry [65]; reflectometry [66]; and ellipsometry [67]. …”

Section: Introductionmentioning

confidence: 99%

Label-Free Microcavity Biosensors: Steps towards Personalized Medicine

Amarie

Glazier

2012

Sensors

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Personalized medicine has the potential to improve our ability to maintain health and treat disease, while ameliorating continuously rising healthcare costs. Translation of basic research findings to clinical applications within regulatory compliance is required for personalized medicine to become the new foundation for practice of medicine. Deploying even a few of the thousands of potential diagnostic biomarkers identified each year as part of personalized treatment workflows requires clinically efficient biosensor technologies to monitor multiple biomarkers in patients in real time. This paper discusses a critical component of a regulatory system, a microcavity optical biosensor for label-free monitoring of biomolecular interactions at physiologically-relevant concentrations. While most current biosensor research focuses on improving sensitivity, this paper emphasizes other characteristics a biosensor technology requires to be practical in a clinical setting, presenting robust microcavity biosensors which are easy to manufacture and integrate with microfluidics into flexible and redesignable platforms making the microcavity biosensors deployable for continuous monitoring of biomarkers in body fluids in the clinic, in dense 2D random arrays for high-throughput applications like drug-library screening in interactomics, and of the secretory behavior of single cells in the laboratory.

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“…After tip coating, the optrode was allowed to cure overnight. The optrode was then operated as a frequency domain fluorescent lifetime sensor (Bambot et al, 1994;Gewehr and Delpy, 1993;Liao et al, 1997;Papkovsky et al, 1993;Rodriguez et al, 1999;Thar et al, 2001) and calibrated against known oxygen solutions. These calibration solutions were made from deionized water that had been bubbled with gas mixtures with oxygen concentrations ranging from 100% to 0%, with the remainder of the gas mixture being nitrogen.…”

Section: Measuring Rob Profilesmentioning

confidence: 99%

Development of a Biomimetic Root Oxygen Bioavailability Sensor

Porterfield¹,

French²,

DeCarlo³

2008

Biological Engineering

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Low oxygen availability in soil can impair root function, thereby decreasing agronomic productivity. Without oxygen to support mitochondrial respiration, energy levels in roots may limit mineral nutrient (N, P, K) transport. Traditional methods for measuring soil oxygenation include the use of redox electrodes and polarographic oxygen sensors. These approaches are limited to measuring oxygen concentrations at specific locations and cannot determine the bio-availability of that oxygen to a growing root. An innovative approach has been developed for direct measurement of oxygen bioavailability. Instead of building the sensor to measure oxygen concentrations, it is possible to construct an electrochemical system in a manner that makes it sensitive to changes in oxygen transport and availability in the soil. Furthermore, it is even possible to construct the sensor to match the oxygen consumption characteristics of a specific root tip, which is important because most metabolism and nutrient uptake occurs here. This is accomplished by using a conductive-gel membrane system that allows sensor profiles to be engineered to match the metabolic profiles of specific species. If constructed to these standards, the sensor will biomimetically replicate biophysical oxygen depletion profiles that are analogous to those found in the rhizosphere of a growing root tip. The biomimetic root oxygen bio-availability (ROB) sensor integrates all biotic and abiotic factors in the soil that limit oxygen transport to the root, while providing real-time sensing. This new sensor technology could be used as a research tool or as a closed-loop control system for field and greenhouse irrigation.

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Fibre-optic lifetime-based enzyme biosensor

Cited by 22 publications

References 7 publications

Optical Biosensors

Optical Biosensors

Label-Free Microcavity Biosensors: Steps towards Personalized Medicine

Development of a Biomimetic Root Oxygen Bioavailability Sensor

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