Fibre-optic probe for fluorescence diagnostics with blood influence compensation

Zherebtsov, Evgeny; Dremin, Viktor; Zharkikh, Elena V.; Zherebtsova, Angelina I.; Rafailov, Ilya E.; Dunaev, Andrey V.; Bazieva, Natalia; Рафаилов, Э.У.

doi:10.1117/12.2290431

Cited by 10 publications

(9 citation statements)

References 14 publications

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“…The measurements were carried out by the laser Doppler flowmeter LAKK-02 (SPE LAZMA Ltd., Russia) with laser probing radiation of 1064 nm. The calibrated pressure was applied to skin by use of an in-house designed tool (1 in the Fig.1), [8,6] which allowed one to distribute the weight of placed leads (4) over the LDF probe (2) installed into the parallel hole (3). During the experiment the probe tip (5) was the only area of contact with skin.…”

Section: Protocol Designmentioning

confidence: 99%

Optical probe pressure effects on cutaneous blood flow

Mizeva

Potapova

Dremin

et al. 2019

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The variation of blood flow characteristics caused by the probe pressure during noninvasive studies is of particular interest within the context of fundamental and applied research. It has been shown previously that the weak local pressure induces vasodilation, whereas the increased pressure is able to stop the blood flow in the compressed area, as well as to significantly change optical signals.The blood flow oscillations measured by laser Doppler flowmetry (LDF) characterize the functional state of the microvascular system and can be used for noninvasive diagnostics of its abnormality. This study was intended to identify the patterns of the relationship between the oscillating components of blood flow registered by the LDF method under different levels of pressure applied to an optical fiber probe.For this purpose we have developed an original optical probe capable of regulating the applied pressure. The developed protocol included six sequential records of the blood perfusion at pressure within the 0 to 200 mmHg range with unloading at the last stage.Using wavelet analyses, we traced the variation of energy of oscillations for these records in five frequency bands associated with different vascular tone regulation mechanisms. Six young volunteers of the same age (three males and three females) were included in this preliminary study and the protocol was repeated five times in each volunteer. Totally 30 LDF records were available for the analyses. As expected, the LDF signal increases at weak pressure (30 mmHg) and decreases at increased pressure. The statistically stable amplification of endothelial associated blood flow oscillations under the 90 mmHg pressure allowed us to put forward a hypothesis that the endothelial activity increases. The possible causes of this phenomenon are discussed.

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Section: Protocol Designmentioning

confidence: 99%

Optical probe pressure effects on cutaneous blood flow

Mizeva

Potapova

Dremin

et al. 2019

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“…The components of blood, especially haemoglobin, are good absorbers of optical radiation and one of possible measures to reduce this effect is applying to the probe a moderate pressure. This technique allows one to decrease the blood content in the tissue as well as alleviate the attenuation effect of blood on the fluorescence spectrum [21]. However, in surgery in many cases the pressure applied to the end of optical probe can have negative effects on the soft tissues under study.…”

Section: Resultsmentioning

confidence: 99%

Fiber-Optic System for Intraoperative Study of Abdominal Organs during Minimally Invasive Surgical Interventions

et al. 2019

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The paper presents the results of experimental measurements of endogenous fluorescence and blood perfusion in patients with pathology of the organs of hepatopancreatoduodenal area in vivo. A custom setup combining channels for fluorescence spectroscopy (excitation wavelengths of 365 nm and 450 nm) and laser Doppler flowmetry (1064 nm) with fibre optical probe for nondestructive laparoscopic measurements has been developed and applied during minimally invasive operation procedure. Preliminary measurements with two aforementioned channels have been performed at specified excitation wavelengths. The possibility of obtaining fluorescence spectra and laser Doppler flowmetry signals in vivo during minimally invasive interventions was shown. Obtained data show perspectives of further research on technical and methodological development of optical diagnostic methods for minimally invasive surgery. The obtained results can be used to provide a deeper understanding of pathological processes influence on optical properties of abdominal organs tissues, which will ultimately help surgeons to determine the state of vitality in tissues and mucous membranes directly during the process of surgical intervention.

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“…Further research is required to address these issues. The use of an additionalmeasuring channel such as diffuse reflectance spectroscopy channel is planned to be used to simultaneously evaluate the influence of the variable blood content on the registered fluorescence intensity 22 .…”

Section: Resultsmentioning

confidence: 99%

Optical diagnostics of bile duct tissues state with tumor compression

Kandurova

Dremin²,

Zherebtsov

et al. 2019

Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine

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The paper presents the results of in vivo experimental measurements of the endogenous fluorescence and blood perfusion in common bile duct tissues in obstructive jaundice patients during minimally invasive surgical operations. Nowadays the type of interventions become more widespread for treating hepatopancreatobiliary pathologies. An effective feedback which can be established by one or more optical diagnostic channels integrated into the standard instruments for minimally invasive manipulations would be of particular value to practical surgeons. To collect reference data, an experimental study has been conducted using a fiber optical probe with fluorescence spectroscopy and laser Doppler flowmetry channels. For every measurement, the areas of interest were different areas of common bile duct obstructed by a malignant tumor. Obtained results demonstrated significant variety registered intensities of fluorescence spectra and values of blood perfusion. Tested fiber optical endoscopic system showed sensitivity to the tissue state in the investigated area, so the two modal approach seems to be promising for further research and implementation in clinical practice.

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Fibre-optic probe for fluorescence diagnostics with blood influence compensation

Cited by 10 publications

References 14 publications

Optical probe pressure effects on cutaneous blood flow

Optical probe pressure effects on cutaneous blood flow

Fiber-Optic System for Intraoperative Study of Abdominal Organs during Minimally Invasive Surgical Interventions

Optical diagnostics of bile duct tissues state with tumor compression

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