Fibrin Plate Method with Reagents Purified by Affinity Chromatography and its Use for Determination of Fibrinolytic and Other Proteolytic Activity in Saliva, Bile and Plasma

Norén, Ingrid; Ramström, Göran; Wallén, Per

doi:10.1159/000214094

Cited by 25 publications

(17 citation statements)

References 14 publications

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“…Fibrinolytic Activity Preparation of the fibrin plate (Modified Noren Methods) 16) : A 0.3% fibrinogen solution (5 ml) was added to 5 ml of 1% agarose solution, then 0.1 ml of 100 IU/ml thrombin was added to this mixture with or without plasminogen.…”

Section: General Proceduresmentioning

confidence: 99%

Hematological Studies on Black Cumin Oil from the Seeds of Nigella sativa L.

Enomoto

Asano

Iwahori

et al. 2001

Biological & Pharmaceutical Bulletin

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The methanol soluble portion of black cumin oil, which is prepared by compression of seeds of Nigella sativa L., showed inhibitory effects on arachidonic acid (AA)-induced platelet aggregation and blood coagulation. By bioactive assay of AA-induced platelet aggregation, the methanol soluble part was purified to isolate a new compound 2-(2-methoxypropyl)-5-methyl-1,4-benzenediol (1) and two known compounds, thymol (2), carvacrol (3), having very strong inhibitory activity. Further, we then examined the isolated compounds (1-3) and eight related compounds by the screening test for AA-induced platelet aggregation. Compounds possessing aromatic hydroxyl and acetoxyl group had more potent activity than aspirin, which is well known as a remedy for thrombosis.

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Section: General Proceduresmentioning

confidence: 99%

Hematological Studies on Black Cumin Oil from the Seeds of Nigella sativa L.

Enomoto

Asano

Iwahori

et al. 2001

Biological & Pharmaceutical Bulletin

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“…(0-37 (0-07) vs 0-56 (0 06) PU, p < 0-05). The Analysis of individual data on male patients significantly lower plasminogen activator capacity in showed that 17% had values of factor VIII above female patients compared with that in female con-the 90th percentile of matched controls, 22% had (n = 20) (n = 12) (n = 68) Fibrinogen (g/l) [3][4][5][6][7][8][9][10][11][12][13][14][15][16] ( values of von Willebrand factor above the 90th percentile, and 37-5% had values of the fast inhibitor of tissue plasminogen activator about the 90th percentile (values above 135% for factor VIII, 150% for von Willebrand factor, and 3-5 AU/ml for the fast inhibitor of tissue plasminogen activator). Similar analysis of plasminogen activator capacity data showed that 20-8% of male patients had values below the 10th percentile of control subjects (values below 0-15).…”

Section: Clinical Characteristics and Risk Indicatorsmentioning

confidence: 99%

Haemostatic function in myocardial infarction.

Hamsten¹,

Blombäck²,

Wiman³

et al. 1986

Heart

188

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SUMMARY Coagulation factor VIII, von Willebrand factor, antithrombin, fibrinogen, plasminogen activator capacity, and inhibitors of fibrinolysis, including a recently discovered fast inhibitor of tissue plasminogen activator, were measured three to six months after myocardial infarction in 116 male and 32 female patients aged < 45 and in 136 age and sex matched random controls. Plasma concentrations of fibrinogen and the fast inhibitor of tissue plasminogen activator were raised in male patients (with or without correction for orosomucoid levels, blood group distribution, tobacco and alcohol consumption, and weight/height index) and plasminogen activator capacity was reduced. In female patients the concentrations of factor VIII, von Willebrand factor, the fast inhibitor of tissue plasminogen activator, aC2-antiplasmin, and Cl inhibitor were significantly increased. The increase in factor VIII concentrations depended strongly on a persisting inflammatory response. Multivariate analysis indicated that a combination of fibrinogen and tissue plasminogen activator inhibitor concentrations gave the best independent discrimination between male patients and controls. For female patients the best combination was von Willebrand factor and tissue plasminogen activator inhibitor. Male patients with multiple vessel atheromatosis at coronary angiography had higher fibrinogen concentrations than those with atheromatosis of a single vessel. Atheromatosis was defined as sharp-edged, plaque-like, or irregular indentations, often multiple, into the vessel lumen without features suggesting fibromuscular hyperplasia.Several mechanisms have been suggested to explain the pathogenesis of ischaemic heart disease. According to the thrombogenic theory of atherogenesis,l hypercoagulability may be a prerequisite not only for the development of thrombotic complications but also for the pathogenesis of atherosclerosis. Impaired fibrinolysis at the onset and during the course of myocardial infarction is viewed mainly as a reduction in defensive capacity while coronary occlusion and fibrin formation are under way.

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“…16 Protein C concentration was determined using an enzyme-linked immunosorbent assay (ELISA) method using reagents from Boehringer Mannheim, Mannheim, Germany. 17 Tissue plasminogen activator (tPA) activity was determined by a fibrin plate method, 18 and tPA capacity was measured as the difference in tPA activity before and after 10 minutes of venous stasis at 100 mm Hg (between the systolic and diastolic blood pressures).…”

mentioning

confidence: 99%

Coagulation factors and the increased risk of stroke in nonvalvular atrial fibrillation.

Gustafsson

Blombäck

Britton

et al. 1990

Stroke

248

117

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We studied whether hemostatic abnormalities contribute to the increased risk of stroke in patients with nonvalvular atrial fibrillation. Hemostatic function was studied in four agematched groups: 20 patients with nonvalvular atrial fibrillation and a previous ischemic stroke, 20 patients with nonvalvular atrial fibrillation without a previous stroke, 20 stroke patients with sinus rhythm, and 40 healthy controls. Both groups with nonvalvular atrial fibrillation had significantly higher concentrations of von Willebrand factor, factor VM:C, fibrinogen, D-dlmer (a fibrinolytic product), /3-thromboglobulin, and platelet factor 4; a significantly higher fibrinogen/antithrombin ratio; and significantly higher spontaneous amidolytic activity than the healthy controls. Prekallikrein levels were significantly lower in both groups with nonvalvular atrial fibrillation. Stroke patients with sinus rhythm had normal hemostatic function, normal concentrations of platelet-related factors, and a slightly increased concentration of fibrinopeptide A compared with the healthy controls. Both groups with nonvalvular atrial fibrillation differed from the stroke patients with sinus rhythm as they did from the healthy controls. No difference in hemostatic function was seen between the nonvalvular atrial fibrillation patients with and without a previous ischemic stroke. Thus, alterations in hemostatic function may contribute to the increased risk of stroke in patients with nonvalvular atrial fibrillation. (Stroke 1990^1:47-51) N onvalvular atrial fibrillation (NVAF) afflicts 2-4% of 70-year-old people, and its prevalence increases with age. 1 NVAF is an important risk factor for stroke. In the Framingham Study, NVAF was associated with a 5-6 times higher incidence of stroke compared with an age-, sex-, and blood pressure-matched control group without NVAF. 2Left atrial thrombosis causing stroke by arterial embolism has been thought to be the main pathogenetic mechanism in the association between NVAF and stroke. However, the precise mechanism is difficult to determine in individuals with NVAF. As pointed out in recent studies, a considerable proportion of strokes are probably due to atherothrombosis. 3 -4 Generalized atherosclerosis might be the common cause of both NVAF and stroke, thereby explaining the association. If this is true, it can be assumed that there are higher concen-

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Fibrin Plate Method with Reagents Purified by Affinity Chromatography and its Use for Determination of Fibrinolytic and Other Proteolytic Activity in Saliva, Bile and Plasma

Cited by 25 publications

References 14 publications

Hematological Studies on Black Cumin Oil from the Seeds of Nigella sativa L.

Hematological Studies on Black Cumin Oil from the Seeds of Nigella sativa L.

Haemostatic function in myocardial infarction.

Coagulation factors and the increased risk of stroke in nonvalvular atrial fibrillation.

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