Fibrinogen-binding proteins from Staphylococcus aureus

Bodén, Maria K.; Flock, Jan‐Ingmar

doi:10.1007/978-1-4471-3454-1_7

Cited by 18 publications

(19 citation statements)

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“…Studies on the pathogenesis of device-associated infections must consider the characteristics of the medical device [22]. It has been well established that S. aureus can simultaneously express binding proteins for several host components, such as vitronectin, fibronectin, fibrinogen and heparan sulphate [7,[23][24][25][26]. Although binding of collagen type I, collagen type II, bone sialoglycoprotein, fibronectin, vitronectin and thrombospondin has been described, interactions between CNS and host factors are not fully understood [5,[26][27][28][29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Binding of von Willebrand factor by coagulase-negative staphylococci

Lundberg

Ljungh

2000

Journal of Medical Microbiology

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Coagulase-negative staphylococci (CNS) are the most common infectious microorganisms isolated from prosthetic devices. To determine whether von Willebrand factor (vWF) acts as an adhesin in bacterial recognition, bacterial binding of recombinant vWF (rvWF) was studied. Eleven CNS strains, belonging to S. epidermidis, S. haemolyticus and S. hominis species, bound soluble rvWF, but to a lesser extent than S. aureus. S. epidermidis strain H2-W bound 125 I-labelled rvWF in a dose-dependent manner. The binding could be inhibited by unlabelled rvWF and thrombospondin, but not by fibrinogen, vitronectin or the carbohydrates N-acetylgalactoseamine, D-galactose, D-glucose, and D-fucose. Pre-incubation of rvWF with type I collagen and Arg-Gly-AspSer (RGDS) peptides did not inhibit binding, whereas pre-incubation of rvWF with heparin decreased binding significantly. The interaction between CNS and rvWF was sensitive to proteinase treatment of bacterial cells. CNS strains bound to immobilised rvWF an extent greater or equal to the positive control strain S. aureus Cowan I. rvWF binding structures from bacterial cell wall were detected by immunoblot. Cowan I strain had 140-, 90-and 38-kDa binding molecules. S. haemolyticus strain SM131 and S. epidermidis strain H2-W had two (120 and 60 kDa) and five (120, 90, 60, 52 and 38 kDa) binding molecules, respectively. Similar binding structures were formed when cell wall extracts from these strains were incubated with thrombospondin. These results indicate that specific ligand-receptor interaction between CNS and rvWF may contribute to bacterial adhesion and colonisation on biomaterial surfaces. Heparin-binding domains of rvWF might be the crucial regions for bacterial attachment. rvWF and thrombospondin may recognise similar molecules in staphylococcal cell wall extracts.

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Section: Discussionmentioning

confidence: 99%

Binding of von Willebrand factor by coagulase-negative staphylococci

Lundberg

Ljungh

2000

Journal of Medical Microbiology

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“…Numerous bacteria have been shown to bind a variety of ECM components, some of which have not been identified or characterised at the molecular level. A single MSCRAMM can bind several ECM ligands, whilst bacteria such as S. aureus can express several MSCRAMMs that recognise the same matrix molecule (Boden and Flock, 1989;McDevitt et al, 1994). This type of variation and interactions resemble the ones between eukaryotic integrins and matrix molecules, in which the integrin can bind several different ligands, and one particular ligand may be recognised by several integrins (Ruoslahti, 1991).…”

mentioning

confidence: 99%

An introduction to staphylococcus aureus, and techniques for identifying and quantifying s. aureus adhesins in relation to adhesion to biomaterials: review

Sj²,

Rg³

2002

eCM

245

154

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The ability of Staphylococcus aureus to adhere to the extracellular matrix and plasma proteins deposited on biomaterials is a significant factor in the pathogenesis of orthopaedic-device related infections. S. aureus possesses many adhesion proteins on its surface, but it is not known how they interact with each other to form stable interactions with the substrate.A novel method was developed for extracting adhesins from the S. aureus cell wall, which could then be further analysed. The protocol involves using a FastPrep instrument to mechanically disrupt the cell walls resulting in native cell walls. Ionically and covalently bound proteins were then solubilised using sodium dodecyl sulphate (SDS) and lysostaphin, respectively. Western blot analysis of covalently bound proteins using anti-protein A and anticlumping factor A sera showed that S. aureus produces most surface proteins in early growth, and less in postexponential and stationary growth.Immuno-gold labelling of protein A, and clumping factor A was observed all over the bacteria and showed no distinct surface distribution pattern. However, this labelling showed expression of surface associated proteins varied in a growth-phase dependent and cell-density dependent manner.

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“…About 40% of humans without systemic infection are persistent or intermittent carriers of S. aureus in the nasal mucosa (17,19). Exposure of the immune system to polypeptide antigens via the nasal mucosa can induce mucosal and systemic antibody responses (64,65).…”

Section: Discussionmentioning

confidence: 99%

Constitutive Production of Catalytic Antibodies to a Staphylococcus aureus Virulence Factor and Effect of Infection

Brown

Nishiyama

Dunkle

et al. 2012

Journal of Biological Chemistry

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Background:We examined the role of catalytic antibodies in immune defense against Staphylococcus aureus. Results: IgG from non-infected humans and mice hydrolyzed the S. aureus extracellular fibrinogen-binding protein (Efb), and the activity was reduced following infection. Conclusion: Constitutive but not adaptive production of catalytic antibodies suggests that Efb expresses B-lymphocyte superantigenic character. Significance: Efb superantigenic character may be a factor in S. aureus pathogenesis.

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Fibrinogen-binding proteins from Staphylococcus aureus

Cited by 18 publications

References 4 publications

Binding of von Willebrand factor by coagulase-negative staphylococci

Binding of von Willebrand factor by coagulase-negative staphylococci

An introduction to staphylococcus aureus, and techniques for identifying and quantifying s. aureus adhesins in relation to adhesion to biomaterials: review

Constitutive Production of Catalytic Antibodies to a Staphylococcus aureus Virulence Factor and Effect of Infection

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