Fibrinolytische Aktivit�t der Haut bei Psoriasis und einigen anderen Dermatosen

Hg, Piper; Hadlich, Janaína Conte; Würbach, G

doi:10.1007/bf00499300

Cited by 17 publications

(1 citation statement)

References 15 publications

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“…In the present paper, our modified culture method may have satisfied the necessary conditions shown in the formula of Warburg as well as the factor (F) and thus maintained the psoriatic lesion in vitro. In the present experiments, psoriatic explants maintained not only their morphological characteristics but also their biochemical characteristics, including lowered isoproterenol-stimulated cAMP levels (20) and elevated plasminogen activator activity (21)(22)(23) in the involved psoriatic epidermis.…”

Section: Skin Explantsmentioning

confidence: 72%

Maintenance of Epidermal Structures of Psoriatic Skin in Organ Culture

Kondô

1986

The Journal of Dermatology

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A method for maintaining the epidermal structures of the normal human and uninvolved and involved psoriatic skin explants in organ culture was described. Skin explants were put in diffusion chambers made of millipore filters, which were then submitted to a roller tube culture at 15 rpm and 32°C. With a gas phase of air + 5% CO2 (phase A), both normal and psoriatic skin explants in culture showed parakeratosis or degeneration of their epidermal cells; with a gas phase of either 50% O2 + 45% N2 + 5% CO2 (phase B) or 95% O2 + 5% CO2 (phase C), they showed neither parakeratosis nor degeneration of epidermal cells and maintained their original structures for 14 days. Autoradiographically, the number of thymidine‐3H (3H‐TdR) labeled epidermal cells in organ culture was greater in involved psoriatic skin explants than in uninvolved ones. Isoproterenol‐stimulated cyclic AMP (cAMP) levels were lower in involved psoriatic epidermis of skin explants than in uninvolved ones, and plasminogen activator activity was higher in the former than in the latter. Addition of dexamethasone normalized the keratinization of the involved psoriatic explant in culture, but addition of dibutyryl cyclic AMP (db cAMP) and dibutyryl cyclic GMP (db cGMP) did not.

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Section: Skin Explantsmentioning

confidence: 72%