Fibroblast growth factor-2 stimulates adipogenic differentiation of human adipose-derived stem cells

Kakudo, Natsuko; Shimotsuma, Ayuko; Kusumoto, Kenji

doi:10.1016/j.bbrc.2007.05.070

Cited by 120 publications

(78 citation statements)

References 16 publications

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“…2 ). Interestingly, culture of rat BM MSCs or human ASCs in the presence of FGF2 prior to adipogenic induction upregulates expression of PPAR ␥ and enhances subsequent adipogenesis ( 224,225 ). Indeed, exogenous FGF2 facilitates in vivo WAT formation by isolated human SVCs ( 226 ).…”

Section: Beyond Adipose Tissue: Potential Origins Of Ascsmentioning

confidence: 99%

Adipose tissue stem cells meet preadipocyte commitment: going back to the future

Cawthorn

Scheller

MacDougald

2012

Journal of Lipid Research

372

323

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with ill health. Such stigmatization distracts from a fascinating aspect of WAT biology: its enormous plasticity as an organ. Indeed, WAT is capable of massive expansion and contraction in response to chronic alterations in energy balance, accounting for as little as 5% body mass in extremely lean athletes ( 1 ) or as much as 60% body mass in morbidly obese individuals ( 2 ). Moreover in rodents, rabbits, and humans, WAT can regenerate following lipectomy ( 3-6 ). This striking degree of plasticity is unique among organs in adults. On a cellular level, WAT expansion is driven by both hypertrophy and hyperplasia of adipocytes ( 7-13 ). Even in nonexpanding WAT, adipocytes renew frequently to compensate for adipocyte death, with approximately 10% of adipocytes renewed annually ( 14,15 ). These data, which indicate that committed adipocyte progenitors (preadipocytes) exist within WAT, are the product of decades of research motivated largely by our desire to understand adipose tissue in the context of obesity and related diseases. Preadipocytes exist in adipose tissueThough the notion of obesity as a disease has existed since at least the time of Hippocrates ( 16 ), investigation into the origins of the adipocyte and the individual contributions of adipocyte hypertrophy and hyperplasia to adipose bulk began in earnest only after the appearance of the stem cell concept, as related to blood cells, in the early 1900s ( 17 ). In the 1940s, histologic observations of the appearance of adipocytes in chambers implanted in the ears of live rabbits suggested that de novo fat formation was Abstract White adipose tissue (WAT) is perhaps the most plastic organ in the body, capable of regeneration following surgical removal and massive expansion or contraction in response to altered energy balance. Research conducted for over 70 years has investigated adipose tissue plasticity on a cellular level, spurred on by the increasing burden that obesity and associated diseases are placing on public health globally. This work has identifi ed committed preadipocytes in the stromal vascular fraction of adipose tissue and led to our current understanding that adipogenesis is important not only for WAT expansion, but also for maintenance of adipocyte numbers under normal metabolic states. At the turn of the millenium, studies investigating preadipocyte differentiation collided with developments in stem cell research, leading to the discovery of multipotent stem cells within WAT. Such adipose tissue-derived stem cells (ASCs) are capable of differentiating into numerous cell types of both mesodermal and nonmesodermal origin, leading to their extensive investigation from a therapeutic and tissue engineering perspective. However, the insights gained through studying ASCs have also contributed to more-recent progress in attempts to better characterize committed preadipocytes in adipose tissue. Thus, ASC research has gone back to its roots, thereby expanding our knowledge of preadipocyte commitment and adipose tissue biology. -Cawthorn, W. P., E. ...

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Section: Beyond Adipose Tissue: Potential Origins Of Ascsmentioning

confidence: 99%

Adipose tissue stem cells meet preadipocyte commitment: going back to the future

Cawthorn

Scheller

MacDougald

2012

Journal of Lipid Research

372

323

View full text Add to dashboard Cite

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“…It has been extensively studied in the literature and is found to be involved in numerous cellular functions in various cell types, including angiogenesis, tumorigenesis, cell proliferation, differentiation, wound healing, limb formation, and tissue remodeling (Bodo et al, 2002;Bobick et al, 2007;Douwes Dekker et al, 2007;Kakudo et al, 2007;Kanayama et al, 2007;Pratsinis and Kletsas, 2007;Schmal et al, 2007;Choi et al, 2008). In chondrocytes, the role of bFGF as an important cell growth regulatorin the growth plate is already well established (Kilkenny and Hill, 1996).…”

Section: Basic Fgf (A) Actions Of Bfgf In Articular Cartilagementioning

confidence: 99%

Biological impact of the fibroblast growth factor family on articular cartilage and intervertebral disc homeostasis

Ellman

Muddasani

et al. 2008

Gene

152

131

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“…Moreover, since the exposure of adipose-derived stem cells to FGF-2 before the induction of differentiation enhanced the adipogenesis (Kakudo et al 2007), the treatment by FGF-2 before induction may increase the osteogenic and chondrogenic differentiation potentials of hMSCs. In this study, we extracted 714 genes that were up-regulated or down-regulated by FGF-2 (Fig.…”

Section: A B C E Dmentioning

confidence: 99%

“…Fibroblast growth factor-2 (FGF-2) is involved in angiogenesis and tissue repair. It was reported that the abilities of hMSCs to differentiate into osteoblasts and chondrocytes decreased with long-term subculture in vitro; however, FGF-2 increased the potential for osteogenic, chondrogenic, and adipogenic differentiation of human MSCs (hMSCs) (Kakudo et al 2007;Quarto et al 2006;Solchaga et al 2005;Tsutsumi et al 2001). Our previous studies showed that the ability of hMSCs proliferation was decreased by long-term subculture in vitro, during which the expression level of transforming growth factor-b (TGF-b) mRNA was increased (Sawada et al 2006).…”

Section: Introductionmentioning

confidence: 99%

FGF-2 increases osteogenic and chondrogenic differentiation potentials of human mesenchymal stem cells by inactivation of TGF-β signaling

et al. 2007

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Human mesenchymal stem cells (hMSCs) are able to self-replicate and differentiate into a variety of cell types including osteoblasts, chondrocytes, adipocytes, endothelial cells, and muscle cells. It was reported that fibroblast growth factor-2 (FGF-2) increased the growth rate and multidifferentiation potentials of hMSCs. In this study, we investigated the genes involved in the promotion of osteogenic and chondrogenic differentiation potentials of hMSCs in the presence of FGF-2. hMSCs were maintained in the medium with FGF-2. hMSCs were harvested for the study of osteogenic or chondrogenic differentiation potential after 15 days' culture. To investigate osteogenic differentiation, the protein levels of alkaline phosphatase (ALP) and the mRNA expression levels of osteocalcin were measured after the induction of osteogenic differentiation. Moreover, the investigation for chondrogenic differentiation was performed by measuring the mRNA expression levels of type II and type X collagens after the induction of chondrogenic differentiation. The expression levels of ALP, type II collagen, and type X collagen of hMSCs cultured with FGF-2 were significantly higher than control. These results suggested that FGF-2 increased osteogenic and chondrogenic differentiation potentials of hMSCs. Furthermore, microarray analysis was performed after 15 days' culture in the medium with FGF-2. We found that the overall insulin-like growth factor-I (IGF-I) and transforming growth factor-b (TGF-b) signaling pathways were inactivated by FGF-2. These results suggested that the inactivation of IGF-I and TGF-b signaling promotes osteogenic and chondrogenic differentiation potential of hMSCs in the presence of FGF-2.

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Fibroblast growth factor-2 stimulates adipogenic differentiation of human adipose-derived stem cells

Cited by 120 publications

References 16 publications

Adipose tissue stem cells meet preadipocyte commitment: going back to the future

Adipose tissue stem cells meet preadipocyte commitment: going back to the future

Biological impact of the fibroblast growth factor family on articular cartilage and intervertebral disc homeostasis

FGF-2 increases osteogenic and chondrogenic differentiation potentials of human mesenchymal stem cells by inactivation of TGF-β signaling

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