Fibroblast growth factor receptor signaling in kidney and lower urinary tract development

Walker, Keith F.; Sims‐Lucas, Sunder; Bates, Carlton M.

doi:10.1007/s00467-015-3151-1

Cited by 64 publications

(64 citation statements)

References 65 publications

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“…5A). Cluster N6 expressed Spry2, a negative regulator of FGF signalling, with FGF signalling associated with nephron progenitor maintenance (Walker et al, 2016). Notch signalling has recently been shown to regulate early commitment to nephron formation (Chung et al, 2016(Chung et al, , 2017.…”

Section: Mechanisms Regulating Nephron Formation and Maturationmentioning

confidence: 99%

Single cell analysis of the developing mouse kidney provides deeper insight into marker gene expression and ligand-receptor crosstalk

et al. 2019

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to clarify that statements made comparing this and previous studies relate to the published analyses of data within those studies and not the detection of genes within the datasets themselves. The changes made are shown below and both the online full-text and PDF versions have been updated. In the Introduction, the following statement was changed: Original However, existing datasets have apparently not provided the transcriptional depth to identify the signalling pathways that operate within the human fetal kidney, and fail to detect several known ligand and receptor expression patterns in mouse. Corrected The analyses performed on existing datasets have not comprehensively identified the signalling pathway components known to be operating within the mouse fetal kidney. In the Discussion, the following statements were changed: Original For example, although >20,000 cells were profiled at P1 (Adam et al., 2017), several known signalling molecules with functionally validated roles in the nephrogenic niche such as Gdnf, Fgf20, Fgf9, Bmp7, Wnt4 and Fgf8 were not detected in that analysis, precluding further insight into signalling interactions. Corrected Although >20,000 cells were profiled at P1 (Adam et al., 2017), several known signalling molecules with functionally validated roles in the nephrogenic niche were not highlighted in that analysis. Original The improved resolution of gene expression in our study may be due to sequencing depth (∼3000 genes detected per cell), biological replication and differential expression analysis with the edgeR method, which has recently been shown to be a top performer in a comparison of 36 differential expression analysis methods for scRNA-seq data (Soneson and Robinson, 2018). Corrected The improved analysis of signalling interactions provided in this study may be due to sequencing depth (∼3000 genes detected per cell), biological replication and differential expression analysis with the edgeR method, which has recently been shown to be a top performer in a comparison of 36 differential expression analysis methods for scRNA-seq data (Soneson and Robinson, 2018).

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Section: Mechanisms Regulating Nephron Formation and Maturationmentioning

confidence: 99%

Single cell analysis of the developing mouse kidney provides deeper insight into marker gene expression and ligand-receptor crosstalk

et al. 2019

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“…5B). With respect to cluster 6, Sprouty2 is a negative regulator of FGF signalling, with FGF signalling associated with nephron progenitor maintenance (Walker et al, 2016). Notch signalling has recently been shown to regulate early commitment to nephron formation (Chung et al, 2016;Chung et al, 2017).…”

Section: Identification Of a Novel Nephron Progenitor State In Mouse mentioning

confidence: 99%

High throughput single cell RNA-seq of developing mouse kidney and human kidney organoids reveals a roadmap for recreating the kidney

Phipson

Zappia

et al. 2017

Preprint

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Recent advances in our capacity to differentiate human pluripotent stem cells to human kidney tissue are moving the field closer to novel approaches for renal replacement. Such protocols have relied upon our current understanding of the molecular basis of mammalian kidney morphogenesis. To date this has depended upon population based-profiling of non-homogenous cellular compartments. In order to improve our resolution of individual cell transcriptional profiles during kidney morphogenesis, we have performed 10x Chromium single cell RNA-seq on over 6000 cells from the E18.5 developing mouse kidney, as well as more than 7000 cells from human iPSC-derived kidney organoids. We identified 16 clusters of cells representing all major cell lineages in the E18.5 mouse kidney. The differentially expressed genes from individual murine clusters were then used to guide the classification of 16 cell clusters within human kidney organoids, revealing the presence of distinguishable stromal, endothelial, nephron, podocyte and nephron progenitor populations. Despite the congruence between developing mouse and human organoid, our analysis suggested limited nephron maturation and the presence of 'off target' populations in human kidney organoids, including unidentified stromal populations and evidence of neural clusters. This may reflect unique human kidney populations, mixed cultures or aberrant differentiation in vitro. Analysis of clusters within the mouse data revealed novel insights into progenitor maintenance and cellular maturation in the major renal lineages and will serve as a roadmap to refine directed differentiation approaches in human iPSC-derived kidney organoids.

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“…Fibroblast growth factors (FGFs) are also important in the regulation of UB morphogenesis. The embryonic kidney expresses the fgf7, fgf8, fgf9, fgf10 and fgf20 ligands and the receptors fgfr1, fgfr2 and fgfrl1 (Walker et al 2016). Of all the FGFs, fgf8 forms a positive feedback loop to activate Wnt signaling during nephron differentiation (Grieshammer et al 2005).…”

Section: Collecting Duct Developmentmentioning

confidence: 98%

“…Mutants in these genes present fewer UB branches and fewer UB tips, thereby exhibiting reduced collecting duct bifurcation, which in turn results in kidney hypoplasia (Ohuchi et al 2000;Qiao et al 1999). FGF signaling in the kidney was recently reviewed in detail (Walker et al 2016). …”

Section: Collecting Duct Developmentmentioning

confidence: 99%

Kidney development and perspectives for organ engineering

2017

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Organ transplantation is currently the best strategy for treating end stage renal disease (ESRD) but the numbers of donor kidneys available are not sufficient to meet the needs of the ever-increasing ESRD population. Therefore, developments in the field of tissue engineering are necessary to provide alternative treatments. Decellularization and three-dimensional (3D) bioprinting strategies may serve as attractive novel options. Since successful tissue engineering requires an in -depth understanding of organ development and regulatory pathways, we discuss signaling in renal development and the composition of the renal extracellular matrix before presenting progress in the decellularization and 3D bioprinting fields.

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Fibroblast growth factor receptor signaling in kidney and lower urinary tract development

Cited by 64 publications

References 65 publications

Single cell analysis of the developing mouse kidney provides deeper insight into marker gene expression and ligand-receptor crosstalk

Single cell analysis of the developing mouse kidney provides deeper insight into marker gene expression and ligand-receptor crosstalk

High throughput single cell RNA-seq of developing mouse kidney and human kidney organoids reveals a roadmap for recreating the kidney

Kidney development and perspectives for organ engineering

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