Fibroblast-myocyte interactions in in vitro cardiomyogenesis

Groß, Wolfgang

doi:10.1016/0014-4827(82)90376-7

Cited by 7 publications

(2 citation statements)

References 36 publications

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“…Additionally, there may be direct cellcell interactions that are not addressed here. For example, chick heart fibroblasts have been shown to physically move cardiac muscle cells, organizing them into multicellular beating units in mixed cultures, a phenomenon involving direct cell-cell contact (Gross, 1982).…”

Section: Effects Of Growth Factorsmentioning

confidence: 99%

A growth factor for cardiac myocytes is produced by cardiac nonmyocytes.

1991

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Cardiac nonmyocytes, primarily fibroblasts, surround cardiac myocytes in vivo. We examined whether nonmyocytes could modulate myocyte growth by production of one or more growth factors. Cardiac myocyte hypertrophic growth was stimulated in cultures with increasing numbers of cardiac nonmyocytes. This effect of nonmyocytes on myocyte size was reproduced by serum-free medium conditioned by the cardiac nonmyocytes. The majority of the nonmyocyte-derived myocyte growth-promoting activity bound to heparin-Sepharose and was eluted with 0.75 M NaCl. Several known polypeptide growth factors found recently in cardiac tissue, namely acidic fibroblast growth factor (aFGF), basic FGF (bFGF), platelet-derived growth factor (PDGF), tumor necrosis factor alpha (TNF alpha), and transforming growth factor beta 1 (TGF beta 1), also caused hypertrophy of cardiac myocytes in a dose-dependent manner. However, the nonmyocyte-derived growth factor (tentatively named NMDGF) could be distinguished from these other growth factors by different heparin-Sepharose binding profiles (TNF alpha, aFGF, bFGF, and TGF beta 1) by neutralizing growth factor-specific antisera (PDGF, TNF alpha, aFGF, bFGF, and TGF beta 1), by the failure of NMDGF to stimulate phosphatidylinositol hydrolysis (PDGF and TGF beta 1), and, finally, by the apparent molecular weight of NMDGF (45-50 kDa). This nonmyocyte-derived heparin-binding growth factor may represent a novel paracrine growth mechanism in myocardium.

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Section: Effects Of Growth Factorsmentioning

confidence: 99%

A growth factor for cardiac myocytes is produced by cardiac nonmyocytes.

1991

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“…After 11 days in culture, proportional summation of the rates of glucose usage by enriched myocytes and enriched fibroblasts is significantly lower than for mixed cells in culture of the same cell density, suggesting that a functional synergism is established between the cell types that may be reflected in the metabolic behavior. This proposal is strengthened by a recent report (Gross, 1982) in which the interaction between fibroblasts and myocytes from 8-day-old chick embryo heart was monitored cinematographically. Gross concluded that a n obligatory six-step process of fibroblast (or other nonmuscle cell)-myocyte interaction was imperative in obtaining synchronously contracting cardiac muscle cells.…”

Section: Substrate Utilization In Mixed and Enriched Culturesmentioning

confidence: 90%

Myocytes and fibroblasts exhibit functional synergism in mixed cultures of neonatal rat heart cells

Schroedl

Hartzell

1983

Journal Cellular Physiology

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Cardiac cells obtained from neonatal rat heart contain a mixed population of cell types that can be enriched in culture in either myocytes or fibroblast-like cells. A metabolic comparison of mixed heart cell cultures with enriched cultures of the same age-in-culture and initial cell density showed that mixed cultures used glucose more rapidly than either enriched myocytes or fibroblasts. Mixed cultures were shown to respond to deprivation of insulin and of serum with decreases in the rate of glucose usage and decreases in the protein content of cells, whereas enriched cultures did not respond in the expected manner to insulin deprivation. Mixed, 11-day-old cells also exhibited greater increases in cellular protein and greater resistance to the stress of starvation than enriched cultures. Palmitate usage, however, was similar in all cultures examined. We conclude that mixed cultures may serve as a better model system to study cardiac metabolism and to monitor the effects of drugs and hormones on the neonatal myocardium. In addition, it is clear from our results that myocytes and fibroblastic-like cells coexist in a metabolically functional synergism.

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Regulation of the growth of nonmuscle heart cells in culture

Klein

Daood

1985

In Vitro Cell Dev Biol

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Primary cultures of neonatal rat hearts contain both striated muscle (myocytes) as well as nonmuscle heart cells (NMHC). Although myocytes do not divide in culture, NMHC do increase in number. The growth of NMHC is dependent on the concentration of serum in the media over a range of 1 to 10%. When compared to growth in 10%, cells in 1% serum have a prolonged doubling time and reach a maximum density that is 70% less. Thus, 1% serum which supports normal myocyte development is a useful culture media to also maintain muscle heart cell homogeneity by its failure to support optimum NMHC division.

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Fibroblast-myocyte interactions in in vitro cardiomyogenesis

Cited by 7 publications

References 36 publications

A growth factor for cardiac myocytes is produced by cardiac nonmyocytes.

A growth factor for cardiac myocytes is produced by cardiac nonmyocytes.

Myocytes and fibroblasts exhibit functional synergism in mixed cultures of neonatal rat heart cells

Regulation of the growth of nonmuscle heart cells in culture

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