Fibrogenesis and epithelial coating of skin wounds in rats treated with angico extract (Anadenanthera colubrina var. cebil)

Abstract

PURPOSE:To evaluate the effects of angico bark extract (Anadenanthera colubrina var. cebil) in the healing process of the skin of rats. METHODS:Twenty adult male rats were divided into four groups of five animals each, according to the respective postoperative days, as follow: G4, G7, G14 and G21. Each group received two incisions on skin and subcutaneous tissue in the right and left antimere of the thoracic region, separated by a distance of 2 cm. The right lesion was treated daily with saline and the left with the angico alcoholic extract (5%). At the end of each experimental period, the animals were euthanized and fragments of the wound area with the edges were removed, fixed in 10% formaldehyde solution and processed for paraffin embedding. Histological sections (5 µm of thickness) were stained with hematoxylin and eosin (HE), Gomori trichromic and picrosisirus red for morphological and morphometric analyses.Statistical analysis was done by ANOVA and Tukey-Kramer test (p<0.05). RESULTS:Morphological analysis showed larger fibroblasts and a higher concentration of collagen fibers in skyn wounds treated with the angico extract. Morphometric analysis demonstrated a significant increase in the number of fibroblasts at 7th and collagen in 7th and 14th days (p<0.01) in wounds treated with the angico extract. CONCLUSION:The angico alcoholic extract (Anadenanthera colubrina var. cebil) induces the acceleration of wound healing in skin wounds of rats.

Comparison of collagen content in skin wounds evaluated by biochemical assay and by computer-aided histomorphometric analysis

“…The authors demonstrated the similarity of the two methods. Pessoa et al (2015) also used GT and PR staining to evaluate new collagen in rat skin samples. The authors reported good efficacy of the method to achieve realistic and satisfactory data.…”

Periplaneta americana Extracts Promote Skin Wound Healing via Nuclear Factor Kappa B Canonical Pathway and Extracellular Signal‐Regulated Kinase Signaling

“…These processes are closely correlated with the increased activation of NF- κ B and ERK signaling pathways. Furthermore, the enhanced activities of NF- κ B and ERK pathways may represent major molecular targets of PAE, which accelerate wound healing by upregulating the expression of a series of genes involved in cell proliferation, fibrogenesis, reepithelialization, and remodeling [2, 29, 30]. …”

The Potential of Plant Phenolics in Prevention and Therapy of Skin Disorders

“…During the final stage of wound healing, granulation tissue changes into mature scar tissue as a result of resorption of exudate and strong collagen synthesis. The healing process depends on the individual characteristics of an organism, regenerative capacity and the scale of damage and can last from several weeks to several months [ 292 , 293 , 294 ].…”