Fibronectin adsorption on Fe–Cr alloy studied by XPS

Abstract

Improved knowledge of the chemical composition of biomaterial surfaces and a better understanding of interactions between biomolecules and material surfaces will allow a more rational design of biomaterials. Surface characterisation of alloys before and after their interaction with proteins is an essential aspect of this approach. In this work, the interactions of fibronectin with stainless steel surfaces (Fe-17Cr alloy) were studied by X-ray photoelectron spectroscopy (XPS

Evaluation of alkaloids extract of Retama monosperma (L.) Boiss. stems as a green corrosion inhibitor for carbon steel in pickling acidic medium by means of gravimetric, AC impedance and surface studies

“…The peak at lower bending energy (707.2 eV) is attributed to metallic iron as previously reported [39], while that at 711.1 eV assigned to Fe 3+ as mentioned in [40], is associated to ferric oxide/hydroxide species such as Fe 2 O 3 , Fe 3 O 4 and FeOOH [41]. The last peak at 713.9 eV was partly ascribed to the satellite of Fe(III) [42] and partly related to the presence of a small concentration of FeCl 3 on the steel surface due to the testing environment [43,44]. Indeed, the signal of Cl 2p was observed on the steel surface in presence of inhibitor (Fig.…”

Pre‐treatment of titanium surfaces by fibronectin: in situ adsorption and effect of concentration

“…The isotherms proposed by MacDonald et al on TiO 2 particles [6] are not directly comparable, as they were estimated from concentrations in solutions and plotted as a function of the residual Fn concentration. It is possible to compare with data obtained on Fe-17Cr [15] and on polystyrene (PS) polymers, [19] where the adsorption plateaux were reached for solution concentrations of 60 and 150 mg l −1 , respectively. As regards d Fn (XPS) on the adsorption plateau, the value of 5.5 nm obtained in this work is quite comparable to d Fn (XPS) previously estimated for Fe-17Cr (6.3 nm), [15] or to the values obtained for PS (8.2 nm, surface coverage of 0.7) and for oxidized PS (7.1 nm, surface coverage of 0.8).…”

“…It is possible to compare with data obtained on Fe-17Cr [15] and on polystyrene (PS) polymers, [19] where the adsorption plateaux were reached for solution concentrations of 60 and 150 mg l −1 , respectively. As regards d Fn (XPS) on the adsorption plateau, the value of 5.5 nm obtained in this work is quite comparable to d Fn (XPS) previously estimated for Fe-17Cr (6.3 nm), [15] or to the values obtained for PS (8.2 nm, surface coverage of 0.7) and for oxidized PS (7.1 nm, surface coverage of 0.8). [19] Di Milla described the Fn molecule as a cylinder of 60 nm in length and 6 nm in base diameter.…”

“…For the blank test, a very small contribution at 400.4 ± 0.1 eV is also detected, attributed to an organic nitrogen-containing contamination. [15] The C 1s XPS core level spectra obtained after protein adsorption (not shown) were fitted with three contributions (see details in Ref. [11]) corresponding to well-identified carbon bonds present in the Fn molecule.…”

Effect of microgrooved surface topography on osteoblast maturation and protein adsorption

“…Fibronectin is one the most important adhesive proteins of the ECM, consisting of two monomeric units joined together by disulphide bonds close to the carboxyl terminus of the protein to form the characteristic dimeric structure. It is well known that sulphur forms strong complexes with metal elements and its stability on stainless steel after adsorption has been demonstrated via XPS analysis 24 .…”