Pre‐treatment of titanium surfaces by fibronectin: in situ adsorption and effect of concentration

Abstract

In this work, kinetics data on adsorption of fibronectin (Fn) on passivated Ti surfaces (Ti quartz crystals) in phosphate buffered saline (PBS) solution were obtained from in situ investigations with electrochemical quartz crystal microbalance (EQCM): from experiments performed at 15 and 60 mg l −1 Fn solution concentrations, the maximum adsorption was reached within 30 min, and Fn adsorption seemed to be partially reversible, as about 15% of the initially adsorbed protein were removed upon PBS rinsing. The remaining amount of adsorbed Fn was around 1000 ng cm −2 and was similar for both protein concentrations in solution. Moreover, differences in kinetics were observed between the two concentrations: the initial adsorption rate was much larger at 60 mg l −1 than at 15 mg l −1 . Ex situ X-ray photoelectron spectroscopy (

Oxygen plasma immersion ion implantation treatment enhances the human bone marrow mesenchymal stem cells responses to titanium surface for dental implant application

“…In fact, fibronectin is one of the first cell‐binding proteins produced by osteoblasts, which makes it an ideal experimental material for testing the protein adsorption of Ti specimens (Payet et al. ). Our results demonstrate that fibronectin more effectively adsorbs to O‐PIII‐treated T M specimens than to the other Ti specimens.…”

Adsorption behavior of human plasma fibronectin on hydrophobic and hydrophilic Ti6Al4V substrata and its influence on bacterial adhesion and detachment

“…Presumably, the major disagreement between these previously obtained data and the present results originates in the different substrata used. Indeed, using TiO 2 surfaces and fibronectin concentrations of 60 and 100 μg/mL in buffer solutions, Hemmersam et al40 and Payet et al41 measured a surface mass densities of 1100 and 1600 ng/cm 2 , respectively, by means of a quartz crystal microbalance with dissipation (QCM‐D). These densities are larger than that obtained in the present study for the initial bulk protein concentration of 200 μg/mL.…”

Understanding small biomolecule‐biomaterial interactions: A review of fundamental theoretical and experimental approaches for biomolecule interactions with inorganic surfaces

“…The combination of in situ kinetics experiments at the macroscopic scale (by QCM) and ex situ surface analyses at the molecular scale (by XPS) provides information about the reversibility of the adsorption process. The amount of adsorbed biomolecules (estimated by different surface models), including an estimation of surface coverage or an equivalent thickness of adsorbed biomolecules as a function of pH, electrochemical potential, and biomolecule concentration in solution have been measured 42, 43. These elements yield a better understanding of the kinetics and mode of adsorption, as well as comparative elements between different materials, but an adsorption mechanism cannot be proposed yet.…”