Fibronectin Adsorption onto Polyelectrolyte Multilayer Films

Ngankam, A Pascal; Mao, Guangzhao; Tassel, Paul R. Van

doi:10.1021/la035479y

Cited by 76 publications

(70 citation statements)

References 62 publications

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“…Ngankam et al 31 showed that the positively charged, PAH terminated PEM films adsorbed denser FN layer and favorably influenced the distribution of FN molecules by inhibiting their aggregation in the adsorption layer when compared to negative-terminal layers. 31 The density and thickness of FN on a PAH-terminated film were roughly double those on a negatively charged PSS-terminated film. It was also shown that the adsorption of the FN onto polyelectrolyte films such as poly(L-lysine) is a more important factor in controlling cell-materials interactions than film charge and layer number.…”

Section: N-pemus Regulate Ecm Adsorptionmentioning

confidence: 99%

N‐isopropylacrylamide‐based thermoresponsive polyelectrolyte multilayer films for human mesenchymal stem cell expansion

Liao

Moussallem

Kim

et al. 2010

Biotechnology Progress

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Human mesenchymal stem cells (hMSCs) are colony-forming unit fibroblasts (CFU-F) derived from adult bone marrow and have significant potential for many cell-based tissue-engineering applications. Their therapeutic potential, however, is restricted by their diminishing plasticity as they are expanded in culture. In this study, we used N-isopropylacrylamide (NIPAM)-based thermoresponsive polyelectrolyte multilayer (N-PEMU) films as culture substrates to support hMSC expansion and evaluated their effects on cell properties. The N-PEMU films were made via layer-by-layer adsorption of thermoresponsive monomers copolymerized with charged monomers, positively charged allylamine hydrochloride (PAH), or negatively charged styrene sulfonic acid (PSS) and compared to fetal bovine serum (FBS) coated surfaces. Surface charges were shown to alter the extracellular matrix (ECM) structure and subsequently regulate hMSC responses including adhesion, proliferation, integrin expression, detachment, and colony forming ability. The positively charged thermal responsive surfaces improved cell adhesion and growth in a range comparable to control surfaces while maintaining significantly higher CFU-F forming ability. Immunostaining and Western blot results indicate that the improved cell adhesion and growth on the positively charged surfaces resulted from the elevated adhesion of ECM proteins such as fibronectin on the positively charge surfaces. These results demonstrate that the layer-by-layer approach is an efficient way to form PNIPAM-based thermal responsive surfaces for hMSC growth and removal without enzymatic treatment. The results also show that surface charge regulates ECM adhesion, which in turn influences not only cell adhesion but also CFU-forming ability and their multi-lineage differentiation potential.

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Section: N-pemus Regulate Ecm Adsorptionmentioning

confidence: 99%

N‐isopropylacrylamide‐based thermoresponsive polyelectrolyte multilayer films for human mesenchymal stem cell expansion

Liao

Moussallem

Kim

et al. 2010

Biotechnology Progress

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“…Intense amide-I and amide-II bands at 1654 and 1547 cm À1 were obtained, which are diagnostic for protein binding or accumulation at the PEM/water interface. According to classical amide-band-assignment papers, [40][41][42][43] these band positions indicate further that HSA is an a-helix-rich protein. No large variations of the band intensities were observed as a function of time in each of the three sets, which suggests rapid sorption processes and adsorption saturation after around an hour.…”

Section: Influence Of Pem Thicknessmentioning

confidence: 99%

In Situ ATR‐FTIR Spectroscopy on the Deposition and Protein Interaction of Polycation/Alginate Multilayers

2010

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Interfacial phenomena often play a key role in the research and development of biomaterials, biomedical devices, pharmaceutical formulations and separation. In that frame, the interaction between proteins and polymer surfaces constitutes an important phenomenon in colloid and materials science, [1][2][3][4][5] where protein sorption is, on the one hand, desired for bioactive applications (e.g., uptake of collagenized implants) and, on the other, should be prevented for bioinert purposes (e.g., clotting on medical devices, membrane fouling) in order to block or delay further bioadhesion cascades.Recently, much theoretical and experimental research, as well as industrial development work, has been focused on electrostatic forces between proteins and charged surfaces. Experimentally, those charged surfaces can be provided by single-polyelectrolyte or polyelectrolyte-brush layers, [6,7] selfassembled monolayers (SAMs) [8,9] with charged end groups and polyelectrolyte multilayers (PEMs). [10,11] This contribution focuses on PEMs and their interactions with a model protein, which has also been extensively studied by Voegel, Schaaf and co-workers, [12][13][14][15][16] Salloum and Schlenoff, [17] Brynda at al., [18] Sukhishvili at al. [19] and Mü ller and co-workers. [20][21][22] Meanwhile it is commonly accepted, that PEM assemblies are well-defined platforms for studying protein sorption due to fundamental aspects, and for generating protein-inert or binding surfaces for several applications like ophthalmic [23] or bone implant related ones. [24] Some concerning questions are still open. One of these questions is dedicated to the location of the protein after its interaction with the PEM system. In that context, certain reports claim migration into the PEM phase [17] and evidence diffusion and embedding to a certain extent in the PEM phase, [13] while other authors claim the interaction of proteins to PEMs to be restricted to the surface. [25] Herein, we would like to give a further contribution to that issue. While in our former work this was already addressed based on PEM systems that were composed of synthetic polyelectrolytes (PELs), [20,22,25,26] herein we report PEM systems that consisted of linear PEL components of natural origin, claimed to be biocompatible. We chose two PEM systems: poly(ethyleneimine)/alginate (PEI/ALG) and chitosan/alginate (CHT/ALG). In situ attenuated-total-reflection Fourier transform infrared (ATR-FTIR) spectroscopy was selected to address both the deposition and the protein interactions related to these PEM systems. An ATR-FTIR-spectroscopy attachment and measurement concept that was introduced by Fringeli [27] was used, which we have applied since the nineties on the characterization of PEM deposition, [20] composition [21] and internal structure, [28] and of the interactions of model polymers and especially PEM films with proteins, peptides and drugs. [20,25,[29][30][31] Experimental Chemicals and SolutionsSodium alginate (ALG) (460 000 g mol À1 ) was purchased from Kelco (San Diego, ...

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“…18,19 Increasingly, polyelectrolyte multilayers (PEMs) are used as bioactive substrata for the study of cell adhesion or phenotype. 14,[20][21][22][23][24][25][26] PEMs are polyelectrolyte complexes fabricated via a layer-by-layer (LbL) assembly process with dilute solutions of positively and negatively charged polymers or by the LbL assembly of weakly interacting hydrogen bond acceptors/donors with polyelectrolyte polymers of complementary polarity. Because the physical properties and film thickness of weak (pHsensitive) PEMs can be controlled with high precision through assembly conditions such as solution pH, these materials find utility in a range of applications including but not limited to cytophilic substrata and cytophobic coatings.…”

Section: Introductionmentioning

confidence: 99%

Biochemical Functionalization of Polymeric Cell Substrata Can Alter Mechanical Compliance

et al. 2006

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Biochemical functionalization of surfaces is an increasingly utilized mechanism to promote or inhibit adhesion of cells. To promote mammalian cell adhesion, one common functionalization approach is surface conjugation of adhesion peptide sequences such as Arg-Gly-Asp (RGD), a ligand of transmembrane integrin molecules. It is generally assumed that such functionalization does not alter the local mechanical properties of the functionalized surface, as is important to interpretations of macromolecular mechanotransduction in cells. Here, we examine this assumption systematically, through nanomechanical measurement of the nominal elastic modulus of polymer multilayer films of nanoscale thickness, functionalized with RGD through different processing routes. We find that the method of biochemical functionalization can significantly alter mechanical compliance of polymeric substrata such as weak polyelectrolyte multilayers (PEMs), increasingly utilized materials for such studies. In particular, immersed adsorption of intermediate functionalization reagents significantly decreases compliance of the PEMs considered herein, whereas polymer-on-polymer stamping of these same reagents does not alter compliance of weak PEMs. This finding points to the potential unintended alteration of mechanical properties via surface functionalization and also suggests functionalization methods by which chemical and mechanical properties of cell substrata can be controlled independently.

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Fibronectin Adsorption onto Polyelectrolyte Multilayer Films

Cited by 76 publications

References 62 publications

N‐isopropylacrylamide‐based thermoresponsive polyelectrolyte multilayer films for human mesenchymal stem cell expansion

N‐isopropylacrylamide‐based thermoresponsive polyelectrolyte multilayer films for human mesenchymal stem cell expansion

In Situ ATR‐FTIR Spectroscopy on the Deposition and Protein Interaction of Polycation/Alginate Multilayers

Biochemical Functionalization of Polymeric Cell Substrata Can Alter Mechanical Compliance

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