Fibronectin adsorption to nanopatterned silicon surfaces

Salakhutdinov, Ildar; VandeVord, Pamela J.; Palyvoda, Olena; Matthew, Howard W.T.; Handa, Hitesh; Mao, Guangzhao; Auner, Gregory W.; Newaz, Golam

doi:10.1364/biomed.2008.bsue11

Cited by 8 publications

(12 citation statements)

References 6 publications

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“…Protein adsorption facilitates the cellular interaction with the substrate surface. The number and strength of cells adhered to a substrate is inherently affected by the level of protein adsorption 26, 37–39. Surface topography has been shown to either directly influence the cell response, or indirectly affect the cell responses through the affects they have on the adsorption of proteins 10, 40–42.…”

Section: Discussionmentioning

confidence: 99%

“…The number and strength of cells adhered to a substrate is inherently affected by the level of protein adsorption. 26,[37][38][39] Surface topography has been shown to either directly influence the cell response, or indirectly affect the cell responses through the affects they have on the adsorption of proteins. 10,[40][41][42] Cell adhesion results comparing substrates coated with fibronectin, collagen, or no protein revealed that on all protein-coated substrates, there was significantly more cell adhesion on both of the protein-coated substrates compared with the substrates without any protein coating.…”

Section: Discussionmentioning

confidence: 99%

“…Literature shows a gap in research of nanotopography influence in the reduction of astrogliosis. Preliminary data collected in this laboratory was utilized in determining the material and nanopattern feature dimensions 26, 27. Nanopatterned surfaces were fabricated with nanoimprint lithography (NIL) on poly(methyl methacrylate) (PMMA) surfaces.…”

Section: Introductionmentioning

confidence: 99%

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Nanopatterning effects on astrocyte reactivity

Ereifej

Matthew

Newaz

et al. 2012

J Biomedical Materials Res

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An array of design strategies have been targeted toward minimizing failure of implanted microelectrodes by minimizing the chronic glial scar around the microelectrode under chronic conditions. Current approaches toward inhibiting the initiation of glial scarring range from altering the geometry, roughness, size, shape, and materials of the device. Studies have shown materials which mimic the nanotopography of the natural environment in vivo will consequently result in an improved biocompatible response. Nanofabrication of electrode arrays is being pursued in the field of neuronal electrophysiology to increase sampling capabilities. Literature shows a gap in research of nanotopography influence in the reduction of astrogliosis. The aim of this study was to determine optimal feature sizes for neural electrode fabrication, which was defined as eliciting a nonreactive astrocytic response. Nanopatterned surfaces were fabricated with nanoimprint lithography on poly(methyl methacrylate) surfaces. The rate of protein adsorption, quantity of protein adsorption, cell alignment, morphology, adhesion, proliferation, viability, and gene expression was compared between nanopatterned surfaces of different dimensions and non-nanopatterned control surfaces. Results of this study revealed that 3600 nanopatterned surfaces elicited less of a response when compared with the other patterned and non-nanopatterned surfaces. The surface instigated cell alignment along the nanopattern, less protein adsorption, less cell adhesion, proliferation and viability, inhibition of glial fibrillary acidic protein, and mitogen-activated protein kinase kinase 1 compared with all other substrates tested.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nanopatterning effects on astrocyte reactivity

Ereifej

Matthew

Newaz

et al. 2012

J Biomedical Materials Res

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“…This protein adsorption facilitates the cellular interaction with the substrate surface. The number and strength of cells adhered to a substrate is inherently affected by the level of protein adsorption 30, 36, 37. Our results found the fewest number of astrocytes on PMMA compared to any other surface, which could be explained by a low amount of protein adsorption on PMMA.…”

Section: Discussionmentioning

confidence: 99%

Characterization of astrocyte reactivity and gene expression on biomaterials for neural electrodes

Ereifej

Khan

Newaz

et al. 2011

J Biomedical Materials Res

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Neural electrode devices hold great promise to help people with the restoration of lost functions. However, research is lacking in the biomaterial design of a stable, long-term device. Glial scarring is initiated when a device is inserted into brain tissue and an inflammatory response ensues. Astrocytes become hypertrophic, hyperplastic, and upregulate glial-fibrillary acidic protein. This study was designed to investigate the astrocyte proliferation, viability, morphology, and gene expression to assess the reactive state of the cells on different material surfaces. Although platinum and silicon have been extensively characterized both in vivo and in vitro for their biocompatibility with neuronal cells, this study used the novel usage of PMMA and SU-8 in neural electrodes by comparative analysis of materials' biocompatibility. This study has shown evidence of noncytotoxicity of SU-8. We have also confirmed the biocompatibility of PMMA with astrocytes. Moreover, we have established sound guidelines of which neural implant materials should meet to be depicted biocompatible.

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“…During recent years, this field is rapidly expanding. One of the new subfields here includes studies of protein adsorption on rough surfaces with well controlled nanoroughness, e.g., on surfaces obtained by using e-gun evaporation at an oblique angle of incidence between the substrate and the incoming flux [4][5][6] or on diffraction-grated surfaces [7]. Another subfield encompasses studies of protein adsorption and DNA hybridization on supported nanoparticles (see, e.g., Refs.…”

Section: Introductionmentioning

confidence: 99%