1979
DOI: 10.1073/pnas.76.6.2644
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Fibronectin and the multiple interaction model for platelet-collagen adhesion.

Abstract: A rapid, sensitive, and reproducible assay to determine the adhesion of platelets to collagen has been developed. Collagen fibers and adherent platelets are retained on polycarbonate membrane filters. Chemical modification of collagen by acetylation and of platelets by treatment with chymotrypsin markedly reduces adhesion. The role of fibronectin in the collagen-platelet interaction has been examined. Treatment of platelets with purified antibody or Fab' fragments to fibronectin only slightly reduces adhesion.… Show more

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Cited by 71 publications
(22 citation statements)
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“…Although only a small portion of the total amount of serum spreading factor in blood is associated with platelets, it is possible that this factor, which mediates phenomena related to cell adhesion in culture, may be-involved in platelet adhesion or aggregation. Fibronectin also has been reported to occur in platelet-associated form, but its importance in platelet function is unclear (21,22).…”
Section: Resultsmentioning
confidence: 99%
“…Although only a small portion of the total amount of serum spreading factor in blood is associated with platelets, it is possible that this factor, which mediates phenomena related to cell adhesion in culture, may be-involved in platelet adhesion or aggregation. Fibronectin also has been reported to occur in platelet-associated form, but its importance in platelet function is unclear (21,22).…”
Section: Resultsmentioning
confidence: 99%
“…Bensusan et al (8) has proposed that fibronectin on the surface of platelets acts as the receptor for collagen, but this has not been confirmed by others (11). Platelets contain fibronectin in their a-granules and release it upon stimulation with thrombin or collagen (37).…”
Section: 7±13 (3)mentioning
confidence: 99%
“…Its role has been disputed by others (11,12), and no direct evidence for a major role of fibronectin in the adhesion of blood platelets to collagen is currently available.…”
Section: Introductionmentioning
confidence: 99%
“…This different behavior of fibronectin can also explain the contrasting evidence re ported in the literature; in fact, some authors [3,4], using different experimental ap proaches, have come to the conclusion that fibronectin changes significantly the extent of adhesion to collagen, while others [2,5] sup port the opinion that this molecule does not play any role in platelet adhesion.…”
Section: Discussionmentioning
confidence: 74%
“…Many biological functions have been proposed for this molecule that seems to play a role in phagocytic activity of macrophages, malignant cell transformation, cellular motil ity, cellular adhesion, and in the formation of hemostatic plug [1], Because platelets physiologically adhere to the exposed subendothelial layer of injured vessel wall, many authors investigated the possible role of fibronectin in mediating this phenomenon. Contrasting evidence has been obtained: Bemusan et al [2] concluded that fibronectin present on platelet membranes is the collagen receptor of the cell; Koteliansky et al [3] demonstrated that preincubation of collagen with fibronectin greatly enhances platelet adhesion independently of the pres ence of this molecule in soluble form in the medium; on the contrary, Santoro and Cun ningham [4] observed only very small changes in adhesion to collagen induced by fibronectin, and Sochynsky et al [5] con cluded that this molecule has no role in plate let adhesion.…”
Section: Introductionmentioning
confidence: 92%