Fibronectin-functionalization of 3D collagen networks supports immune tolerance and inflammation suppression in human monocyte-derived macrophages

Logie, Colin; Schaik, Tom van; Pompe, Tilo; Pietsch, Katja

doi:10.1016/j.biomaterials.2020.120498

Cited by 20 publications

(19 citation statements)

References 56 publications

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“…Long term studies (days or weeks) will increase the significance impact and enhance translational possibilities. Growing evidence points towards a key role of the extracellular matrix in the modulation of the potency of immune cells [52,69,[121][122][123]. Traditional 2D cell culture plastics Growing evidence points towards a key role of the extracellular matrix in the modulation of the potency of immune cells [52,69,[121][122][123].…”

Section: Technological Advances For Future Microgravity Researchmentioning

confidence: 99%

“…Growing evidence points towards a key role of the extracellular matrix in the modulation of the potency of immune cells [52,69,[121][122][123]. Traditional 2D cell culture plastics Growing evidence points towards a key role of the extracellular matrix in the modulation of the potency of immune cells [52,69,[121][122][123]. Traditional 2D cell culture plastics (e.g., microwell plate, Petri dish, and cell culture flask) are unable to resemble the in vivo situation [124,125].…”

Section: Technological Advances For Future Microgravity Researchmentioning

confidence: 99%

See 1 more Smart Citation

May the Force Be with You (Or Not): The Immune System under Microgravity

ElGindi

Sapudom

Ibrahim

et al. 2021

Cells

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All terrestrial organisms have evolved and adapted to thrive under Earth’s gravitational force. Due to the increase of crewed space flights in recent years, it is vital to understand how the lack of gravitational forces affects organisms. It is known that astronauts who have been exposed to microgravity suffer from an array of pathological conditions including an impaired immune system, which is one of the most negatively affected by microgravity. However, at the cellular level a gap in knowledge exists, limiting our ability to understand immune impairment in space. This review highlights the most significant work done over the past 10 years detailing the effects of microgravity on cellular aspects of the immune system.

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Section: Technological Advances For Future Microgravity Researchmentioning

confidence: 99%

Section: Technological Advances For Future Microgravity Researchmentioning

confidence: 99%

May the Force Be with You (Or Not): The Immune System under Microgravity

ElGindi

Sapudom

Ibrahim

et al. 2021

Cells

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“…Studies have shown that scaffolds with orderly 3D network structure can act as more effective support for cell growth than 2D scaffolds by maintaining the uniform distribution of cells inside scaffolds and providing adequate levels of oxygen and nutrients, not only that, 3D porous scaffolds can recruit more giant cells and macrophages, which will be more conducive to assisting diabetic wounds to resist microbial damage [ 17 , 18 ]. Therefore, it is of great importance to improve the porosity of 2D nanofibrous scaffolds in the third dimension while the micropatterned structures of those scaffolds could be maintained very well [ 16 , 19 – 21 ].…”

Section: Introductionmentioning

confidence: 99%

Nanofiber/hydrogel core–shell scaffolds with three-dimensional multilayer patterned structure for accelerating diabetic wound healing

Zhang

Pan

et al. 2022

J Nanobiotechnol

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Impaired angiogenesis is one of the predominant reasons for non-healing diabetic wounds. Herein, a nanofiber/hydrogel core–shell scaffold with three-dimensional (3D) multilayer patterned structure (3D-PT-P/GM) was introduced for promoting diabetic wound healing with improved angiogenesis. The results showed that the 3D-PT-P/GM scaffolds possessed multilayered structure with interlayer spacing of about 15–80 μm, and the hexagonal micropatterned structures were uniformly distributed on the surface of each layer. The nanofibers in the scaffold exhibited distinct core–shell structures with Gelatin methacryloyl (GelMA) hydrogel as the shell and Poly (d, l-lactic acid) (PDLLA) as the core. The results showed that the porosity, water retention time and water vapor permeability of the 3D-PT-P/GM scaffolds increased to 1.6 times, 21 times, and 1.9 times than that of the two-dimensional (2D) PDLLA nanofibrous scaffolds, respectively. The in vitro studies showed that the 3D-PT-P/GM scaffolds could significantly promote cell adhesion, proliferation, infiltration and migration throughout the scaffolds, and the expression of cellular communication protein-related genes, as well as angiogenesis-related genes in the same group, was remarkably upregulated. The in vivo results further demonstrated that the 3D-PT-P/GM scaffolds could not only effectively absorb exudate and provide a moist environment for the wound sites, but also significantly promote the formation of a 3D network of capillaries. As a result, the healing of diabetic wounds was accelerated with enhanced angiogenesis, granulation tissue formation, and collagen deposition. These results indicate that nanofiber/hydrogel core–shell scaffolds with 3D multilayer patterned structures could provide a new strategy for facilitating chronic wound healing. Graphical Abstract

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“…Aberrant AT remodeling can also lead to disorders of adipose tissue secretions, such as cytokines, hormones and metabolites, which can disrupt tissue metabolism and lead to imbalances in cellular metabolism [ 2 , 3 , 4 ]. Studies have found that these changes cause changes in adipose tissue function and are related to extracellular matrix (ECM) remodeling [ 5 , 6 ], and abnormal changes in ECM components will affect the activation of immune cells and the spread of inflammation [ 7 ]. Interestingly, the transcriptome analysis of adipose tissue in obese people found that the genes of ECM components are significantly up-regulated, and this change is closely related to the occurrence of inflammation [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Collagen XV Promotes ER Stress-Induced Inflammation through Activating Integrin β1/FAK Signaling Pathway and M1 Macrophage Polarization in Adipose Tissue

Liu

et al. 2021

IJMS

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Collagen XV (Col XV), a basement membrane (BM) component, is highly expressed in adipose tissue, and studies have found that Col XV is related to extracellular matrix (ECM) remodeling involving in adipose tissue fibrosis and inflammation. Furthermore, the ECM is essential for maintaining normal development and tissue function. In this study, we found that Col XV is related to the endoplasmic reticulum stress (ERS) and inflammation of adipose tissue. Moreover, we found that overexpression of Col XV in mice could cause macrophages to infiltrate white adipose tissue (iWAT). At the same time, the expression of the ERS sensor IRE1α (Inositol-Requiring Enzyme-1α) was significantly up-regulated, which intensified the inflammation of adipose tissue and the polarization of M1 macrophages after the overexpression of Col XV in mice. In addition, after overexpression of Col XV, the intracellular Ca2+ concentration was significantly increased. Using focal adhesion kinase (FAK) inhibitor PF573228, we found that PF-573228 inhibited the phosphorylation of FAK and reversed the upward trend of Col XV-induced protein expression levels of IRE1α, C/EBP-homologous protein (CHOP), and 78 kDa glucose-regulated protein (GRP78). After treatment with IRE1α inhibitor STF-083010, the results showed that the expression of adipocyte inflammation-related genes interleukin 6 (IL-6) and tumor necrosis factor α (TNFα) significantly were decreased. Our results demonstrate that Col XV induces ER-stress in adipocytes by activating the Integrinβ1/FAK pathway and disrupting the intracellular Ca2+ balance. At the same time, Col XV regulates the inflammation induced by ER stress in adipocytes by promoting IRE1α/XBP1 (X-Box binding protein 1) signaling. Our study provides new ideas for solving the problems of adipose tissue metabolism disorders caused by abnormal accumulation of ECM.

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Fibronectin-functionalization of 3D collagen networks supports immune tolerance and inflammation suppression in human monocyte-derived macrophages

Cited by 20 publications

References 56 publications

May the Force Be with You (Or Not): The Immune System under Microgravity

May the Force Be with You (Or Not): The Immune System under Microgravity

Nanofiber/hydrogel core–shell scaffolds with three-dimensional multilayer patterned structure for accelerating diabetic wound healing

Collagen XV Promotes ER Stress-Induced Inflammation through Activating Integrin β1/FAK Signaling Pathway and M1 Macrophage Polarization in Adipose Tissue

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