Fibrous Scaffolds with Varied Fiber Chemistry and Growth Factor Delivery Promote Repair in a Porcine Cartilage Defect Model

KimIris, L; PfeiferChristian, G; FisherMatthew, B; SaxenaVishal,; MeloniGregory, R; KwonMi, Y; KimMinwook,; SteinbergDavid, R; Mauck, Robert L.; BurdickJason, A

doi:10.1089/ten.tea.2015.0150

Cited by 48 publications

(52 citation statements)

References 47 publications

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“…Specifically, nano‐ to micro‐scale polymer fibers can be generated by electrospinning and collected as either random or aligned fibrous networks to mimic the highly organized collagen fibers of these dense connective tissues. Recent advances in biomaterials design and electrospinning techniques have expanded the range of biophysical (e.g., alignment, diameter, and stiffness) and biochemical (e.g., adhesion ligand and growth factor) properties of scaffolds to better explore features that enable expedited tissue repair.…”

Section: Introductionmentioning

confidence: 99%

Influence of Fiber Stiffness on Meniscal Cell Migration into Dense Fibrous Networks

Song

Heo

Peredo

et al. 2019

Adv Healthcare Materials

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Fibrous scaffolds fabricated via electrospinning are being explored to repair injuries within dense connective tissues. However, there is still much to be understood regarding the appropriate scaffold properties that best support tissue repair. In this study, the influence of the stiffness of electrospun fibers on cell invasion into fibrous scaffolds is investigated. Specifically, soft and stiff electrospun fibrous networks are fabricated from crosslinked methacrylated hyaluronic acid (MeHA), where the stiffness is altered via the extent of MeHA crosslinking. Meniscal fibrochondrocyte (MFC) adhesion and migration into fibrous networks are investigated, where the softer MeHA fibrous networks are easily deformed and densified through cellular tractions and the stiffer MeHA fibrous networks support ≈50% greater MFC invasion over weeks when placed adjacent to meniscal tissue. When the scaffolds are sandwiched between meniscal tissues and implanted subcutaneously, the stiffer MeHA fibrous networks again support enhanced cellular invasion and greater collagen deposition after 4 weeks when compared to the softer MeHA fibrous networks. These results indicate that the mechanics and deformability of fibrous networks likely alter cellular interactions and invasion, providing an important design parameter toward the engineering of scaffolds for tissue repair.

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Section: Introductionmentioning

confidence: 99%

Influence of Fiber Stiffness on Meniscal Cell Migration into Dense Fibrous Networks

Song

Heo

Peredo

et al. 2019

Adv Healthcare Materials

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“…Unlike other widely used models, porcine subjects are particularly well suited as a pre-clinical model because the nerves are remarkably similar to humans. 33 Moreover, the similar physiology between humans and porcine have resulted in its increasing popularity as a preclinical translational research model in various areas, such as traumatic brain injury, [34][35][36][37][38][39][40] spinal cord injury, 41 wound healing, 42 cartilage repair, [43][44][45][46][47] intervertebral disc herniation, 48 coronary artery injury, 49 and gastrointestinal, 50 and hepatic surgery. 51 Indeed, the objective in the development of the porcine model presented in this study was to replicate critical features of extremely challenging repair and regeneration scenarios following major PNI in human.…”

Section: Discussionmentioning

confidence: 99%

A Porcine Model of Peripheral Nerve Injury Enabling Ultra-Long Regenerative Distances: Surgical Approach, Recovery Kinetics, and Clinical Relevance

Burrell

Browne

Dutton

et al. 2019

Preprint

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AbstractApproximately 20 million Americans currently experience residual deficits from traumatic peripheral nerve injury. Despite recent advancements in surgical technique, peripheral nerve repair typically results in poor functional outcomes due to prolonged periods of denervation resulting from long regenerative distances coupled with relatively slow rates of axonal regeneration. Development of novel surgical solutions requires valid preclinical models that adequately replicate the key challenges of clinical peripheral nerve injury. Our team has developed a porcine model using Yucatan minipigs that provides an opportunity to investigate peripheral nerve regeneration using different nerves tailored for a specific mechanism of interest, such as (1) nerve modality: motor, sensory, and mixed-modality; (2) injury length: short versus long gap; and (3) total regenerative distance: proximal versus distal injury. Here, we describe a comprehensive porcine model of two challenging clinically relevant procedures for repair of long segmental lesions (≥ 5 cm) – the deep peroneal nerve repaired using a sural nerve autograft and the common peroneal nerve repaired using a saphenous nerve autograft – each featuring ultra-long total regenerative distances (up to 20 cm and 27 cm, respectively) to reach distal targets. This paper includes a detailed characterization of the relevant anatomy, surgical approach/technique, functional/electrophysiological outcomes, and nerve morphometry for baseline and autograft repaired nerves. These porcine models of major peripheral nerve injury are suitable as preclinical, translatable models for evaluating the efficacy, safety, and tolerability of next-generation artificial nerve grafts prior to clinical deployment.

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“…Considering this, Kim et al proposed the production of PCL/hyaluronic acid fibrous scaffolds loaded with transforming growth factor-β3 for cartilage repair of microfractures in a large animal model such as the minipig. After twelve weeks of implantation in vivo, the loaded scaffolds improved histological scores and increased type 2 collagen content and the overall mechanical performance [177]. Core-shell nanofibrous scaffolds were fabricated to encapsulate bovine serum albumin and the recombinant human transforming growth factor-β3 (rhTGF-β3) for tracheal cartilage regeneration.…”

Section: Cartilagementioning

confidence: 99%

Spun Biotextiles in Tissue Engineering and Biomolecules Delivery Systems

et al. 2020

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Nowadays, tissue engineering is described as an interdisciplinary field that combines engineering principles and life sciences to generate implantable devices to repair, restore and/or improve functions of injured tissues. Such devices are designed to induce the interaction and integration of tissue and cells within the implantable matrices and are manufactured to meet the appropriate physical, mechanical and physiological local demands. Biodegradable constructs based on polymeric fibers are desirable for tissue engineering due to their large surface area, interconnectivity, open pore structure, and controlled mechanical strength. Additionally, biodegradable constructs are also very sought-out for biomolecule delivery systems with a target-directed action. In the present review, we explore the properties of some of the most common biodegradable polymers used in tissue engineering applications and biomolecule delivery systems and highlight their most important uses.

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Fibrous Scaffolds with Varied Fiber Chemistry and Growth Factor Delivery Promote Repair in a Porcine Cartilage Defect Model

Cited by 48 publications

References 47 publications

Influence of Fiber Stiffness on Meniscal Cell Migration into Dense Fibrous Networks

Influence of Fiber Stiffness on Meniscal Cell Migration into Dense Fibrous Networks

A Porcine Model of Peripheral Nerve Injury Enabling Ultra-Long Regenerative Distances: Surgical Approach, Recovery Kinetics, and Clinical Relevance

Spun Biotextiles in Tissue Engineering and Biomolecules Delivery Systems

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