Field Application of Nanoliposomes Delivered Quercetin by Inhibiting Specific hsp70 Gene Expression Against Plant Virus Disease

Abstract

The annual economic loss caused by plant viruses exceeds 10 billion dollars due to the lack of ideal control measures. Quercetin is a flavonol compound that exerts a control effect on plant virus diseases, but its poor solubility and stability limit the control efficiency. Fortunately, the development of nanopesticides has led to new ideas. In this study, 117 nm quercetin nanoliposomes with excellent stability were prepared from biomaterials, and few surfactants and stabilizers were added to optimize the formula. Nbhsp70er-1 and Nbhsp70c-A were found to be the target genes of quercetin, through abiotic and biotic stress, and the nanoliposomes improved the inhibitory effect at the gene and protein levels by 33.6% and 42%, respectively. Finally, the results of field experiment showed that the control efficiency was 38% higher than that of the conventional quercetin formulation and higher than those of other antiviral agents. This research is the first to report the combination of biological antiviral agents and nanotechnology to control plant virus diseases, and it significantly improved the control efficiency and reduced the use of traditional chemical pesticides.

Pharmacological Activity of Quercetin: An Updated Review

“…In vitro Quercetin, quercetin-3′-sulfate, and Quercetin has a preventive efect on plant virus infections. Wang et al [80] optimized the prescription by adding a few of surfactants and stabilizers using biomaterials as raw materials to obtain 117 nm quercetin nanoliposomes with good stability. Tey found that Nbhsp70er-1 and Nbhsp70c-A were target genes for quercetin and that nanoliposomes had a 33.6% and 42% increase in inhibition at gene and protein levels, respectively.…”

Antiviral modes of action of the novel compound GLY‐15 containing pyrimidine heterocycle and moroxydine skeleton against tobacco mosaic virus

“…20,21 Studies have shown that quercetin nanoliposomes can inhibit TMV by specifically inhibiting the expression of Hsp70 in N. benthamiana. 22 In addition, a polysaccharide was found in Dendrobium nobile, which can stimulate calcium signaling pathway and promote host defense against TMV infection. 23 In our previous study, 37 new pyrimidine moroxydine derivatives were synthesized by fusing pyrimidine heterocycles and moroxydine skeleton, and their antiviral activities against TMV were tested.…”

“…Polysaccharide peptides, lentinan and ursolic acid can promote the expression of defense‐related enzymes, such as phenylalanine ammonia‐lyase (PAL) and peroxidase (POD), as well as defense‐related genes in host plants, thus increasing the resistance and tolerance of plants to TMV 20,21 . Studies have shown that quercetin nanoliposomes can inhibit TMV by specifically inhibiting the expression of Hsp70 in N. benthamiana 22 . In addition, a polysaccharide was found in Dendrobium nobile , which can stimulate calcium signaling pathway and promote host defense against TMV infection 23 …”