Field application of nanoliposomes delivered quercetin by inhibiting specific hsp70 gene expression against plant virus disease

Wang, Jie; Hao, Kaiqiang; Yu, Fangfei; Song, Lili; Wang, Fenglong; Yang, Jinguang; Su, Chenyu

doi:10.1186/s12951-021-01223-6

Cited by 32 publications

(33 citation statements)

References 61 publications

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“…However, various enzymes and microorganisms in the environment are still potential challenges encountered in applying dsRNA to pest control strategies, especially in the complex environmental conditions in the field. , dsRNA cannot be directly applied to pests, so the selection of a suitable carrier is the key to solving the problem of delivering dsRNA. Liposomes have been repeatedly shown to be carriers with excellent protective properties, and their vesicular structure and amphiphilic membrane structure can effectively prevent the degradation of the active ingredient loaded inside. This study explored the protective performance of LNPs on dsRNA using nucleases that can efficiently degrade dsRNA.…”

Section: Resultsmentioning

confidence: 99%

Delivery of Methoprene-Tolerant dsRNA to Improve RNAi Efficiency by Modified Liposomes for Pest Control

Liu

Sun

et al. 2023

ACS Appl. Mater. Interfaces

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The application of RNA interference (RNAi) technology for pest control is environmentally friendly and accurate. However, the efficiency of RNAi is often inconsistent and unreliable, and finding a suitable carrier element is considered critical to success in overcoming biotic and abiotic barriers to reach the target site. The fall armyworm, Spodoptera frugiperda (FAW), which is one of most important global agricultural pests, has recently spread rapidly to other parts of the world. In this study, a method to improve the stability and RNAi efficiency of the dsRNA carrier complex was reported. Methoprene-tolerant gene (Met) was selected as a target, a gene which is critical to the growth and development of FAW. Biomaterials nanoliposomes (LNPs) were modified with polyethylenimine (PEI) to deliver the dsRNA of Met. The synthesized Met3@PEI@LNPs reached a size of 385 nm and were found to load dsRNA effectively. Through stability and protection assays, it was found that LNPs provided reliable protection. In addition, the release curve also demonstrated that LNPs were able to prevent premature release under alkaline condition of the insect midgut but accelerate the release after entering the acidic environment of the target cells. The cell transfection efficiency of the prepared LNPs reached 96.4%. Toxicity tests showed that the use of LNPs could significantly improve the interference efficiency, with 91.7% interference efficiency achieved when the concentration of dsRNA in LNPs was only 25% of that of the control. Successful interference of Met demonstrated it could significantly shorten the larval period and make the larvae pupate earlier, thus achieving the purpose of control. In this study, we have demonstrated the use of nanotechnology to provide a novel RNAi delivery method for pest control.

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Section: Resultsmentioning

confidence: 99%

Delivery of Methoprene-Tolerant dsRNA to Improve RNAi Efficiency by Modified Liposomes for Pest Control

Liu

Sun

et al. 2023

ACS Appl. Mater. Interfaces

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“…Nanoliposomes were adopted to encapsulate quercetin to efficiently target the phytochemical inside leaf cells through osmosis or endocytosis, overcoming its low solubility which is considered as an important factor limiting the phytopathogen control effect. Nanoliposome-quercetin, indeed, could inhibit the expression of plant hsp70 proteins that are generally induced under stress and which are host factors hijacked by viruses to assemble their replication complex, thus acting as an outperforming antiviral agent even under field conditions [299]. On the other hand, soil-dissolved organic carbon (DOC) strongly reduces flavonoids' lifetime in soil.…”

Section: Discussionmentioning

confidence: 99%

Flavonoids Are Intra- and Inter-Kingdom Modulator Signals

et al. 2022

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Flavonoids are a broad class of secondary metabolites with multifaceted functionalities for plant homeostasis and are involved in facing both biotic and abiotic stresses to sustain plant growth and health. Furthermore, they were discovered as mediators of plant networking with the surrounding environment, showing a surprising ability to perform as signaling compounds for a multitrophic inter-kingdom level of communication that influences the plant host at the phytobiome scale. Flavonoids orchestrate plant-neighboring plant allelopathic interactions, recruit beneficial bacteria and mycorrhizal fungi, counteract pathogen outbreak, influence soil microbiome and affect plant physiology to improve its resilience to fluctuating environmental conditions. This review focuses on the diversified spectrum of flavonoid functions in plants under a variety of stresses in the modulation of plant morphogenesis in response to environmental clues, as well as their role as inter-kingdom signaling molecules with micro- and macroorganisms. Regarding the latter, the review addresses flavonoids as key phytochemicals in the human diet, considering their abundance in fruits and edible plants. Recent evidence highlights their role as nutraceuticals, probiotics and as promising new drugs for the treatment of several pathologies.

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“…Future directions arising from our study include possibilities for exploring the unknown metabolites to discover candidate antiviral compounds that could be utilized in safe and sustainable plant protection. The cysteine precursor L-2-oxo-4-thiazolidine-carboxylic acid and the flavonol quercetin are current examples of control agents with the potential to support the healthy growth of virus-infected plants by alleviating oxidative stress [ 98 , 99 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Poty-Potexvirus Synergism on Growth, Photosynthesis and Metabolite Status of Nicotiana benthamiana

Pollari

Sipari²,

Poque³

et al. 2022

Viruses

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Mixed virus infections threaten crop production because interactions between the host and the pathogen mix may lead to viral synergism. While individual infections by potato virus A (PVA), a potyvirus, and potato virus X (PVX), a potexvirus, can be mild, co-infection leads to synergistic enhancement of PVX and severe symptoms. We combined image-based phenotyping with metabolite analysis of single and mixed PVA and PVX infections and compared their effects on growth, photosynthesis, and metabolites in Nicotiana benthamiana. Viral synergism was evident in symptom severity and impaired growth in the plants. Indicative of stress, the co-infection increased leaf temperature and decreased photosynthetic parameters. In contrast, singly infected plants sustained photosynthetic activity. The host’s metabolic response differed significantly between single and mixed infections. Over 200 metabolites were differentially regulated in the mixed infection: especially defense-related metabolites and aromatic and branched-chain amino acids increased compared to the control. Changes in the levels of methionine cycle intermediates and a low S-adenosylmethionine/S-adenosylhomocysteine ratio suggested a decline in the methylation potential in co-infected plants. The decreased ratio between reduced glutathione, an important scavenger of reactive oxygen species, and its oxidized form, indicated that severe oxidative stress developed during co-infection. Based on the results, infection-associated oxidative stress is successfully controlled in the single infections but not in the synergistic infection, where activated defense pathways are not sufficient to counter the impact of the infections on plant growth.

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Field application of nanoliposomes delivered quercetin by inhibiting specific hsp70 gene expression against plant virus disease

Cited by 32 publications

References 61 publications

Delivery of Methoprene-Tolerant dsRNA to Improve RNAi Efficiency by Modified Liposomes for Pest Control

Delivery of Methoprene-Tolerant dsRNA to Improve RNAi Efficiency by Modified Liposomes for Pest Control

Flavonoids Are Intra- and Inter-Kingdom Modulator Signals

Effects of Poty-Potexvirus Synergism on Growth, Photosynthesis and Metabolite Status of Nicotiana benthamiana

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