Field Assessment of the Effects of Transgenic Rice Expressing a Fused Gene ofcry1Abandcry1AcfromBacillus thuringiensisBerliner on Nontarget Planthopper and Leafhopper Populations

Chen, Mo; Ye, G. Y.; Liu, Z. C.; Yao, Hongwei; Chen, X. X.; Shen, Zhicheng; Hu, Cui; Datta, Swapan K.

doi:10.1603/0046-225x-35.1.127

Cited by 57 publications

(40 citation statements)

References 25 publications

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“…In comparison with the laboratory experiments, our previous multiple yearÐsite studies showed that there were no signiÞcant difference in the population density of brown planthoppers, white-backed planthoppers, and green leafhoppers on Bt rice (B1 and B6, TT9-3, TT9-4, KMD1, and KMD2) and on their non-Bt corresponding controls at various sites (Liu et al 2002(Liu et al , 2003Chen et al 2006bChen et al , 2007Li et al 2007). Likewise, various Bt rice lines with a fusion protein of Cry1Ab/ CpTI (MSA, MSB, MSA4) and their derived hybrid rice lines (21S/MSB, II-32A/MSB, and KF6-304) did not result in consequently higher densities of brown planthoppers, white-backed planthoppers, and green leafhoppers or rice gall midge Orseolia oryzae (WoodMason) (Diptera: Cecidomyiidae) compared with their non-Bt parental control MH86 (Fu et al 2003).…”

Section: Discussionmentioning

confidence: 85%

“…In general, no signiÞcant negative effects of Bt rice on nonlepidopterous herbivores, parasitoids, predators, or soil arthropods have been detected, as measured by indicators of Þtness, population density and dynamics, and biodiversity indices, except when parasitoids used Bt-intoxicated target insects as hosts (Chen et al 2006a(Chen et al , 2009Rahman et al 2007;Cohen et al 2008;Tian et al 2008). Effects of Bt rice on nontarget herbivores have been focused on several piercing-sucking species including the planthoppers, Nilaparvata lugens (Stål), Sogatella furcifera (Horvath), and Laodelphax striatella (Fallé n) (Homoptera: Delphacidae), and the leafhoppers Nephotettix cincticeps (Uhler) and N. virescens (Distant) (Homoptera: Cicadellidae) because Bt proteins may be ingested by these nontarget insects and transported to their natural enemies through tritrophic interactions (Chen et al 2005(Chen et al , 2006b(Chen et al , 2009Bai et al 2006). No adverse effects on the Þtness and population densities of the planthoppers and leafhoppers were observed under laboratory and Þeld conditions in previous studies (Bernal et al 2002;Liu et al 2002Liu et al , 2007Chen et al 2003Chen et al , 2004Chen et al , 2006bFu et al 2003;Bai et al 2006;Zhou et al 2006;Tan et al 2006), except one case in which N. cincticeps actually performed better on Bt rice KMD1 and KMD2 under laboratory and Þeld conditions (Zhou et al 2005).…”

Section: Abstract Bt Rice Nontarget Insect Rice Thrip Stenchaetotmentioning

confidence: 99%

“…Effects of Bt rice on nontarget herbivores have been focused on several piercing-sucking species including the planthoppers, Nilaparvata lugens (Stål), Sogatella furcifera (Horvath), and Laodelphax striatella (Fallé n) (Homoptera: Delphacidae), and the leafhoppers Nephotettix cincticeps (Uhler) and N. virescens (Distant) (Homoptera: Cicadellidae) because Bt proteins may be ingested by these nontarget insects and transported to their natural enemies through tritrophic interactions (Chen et al 2005(Chen et al , 2006b(Chen et al , 2009Bai et al 2006). No adverse effects on the Þtness and population densities of the planthoppers and leafhoppers were observed under laboratory and Þeld conditions in previous studies (Bernal et al 2002;Liu et al 2002Liu et al , 2007Chen et al 2003Chen et al , 2004Chen et al , 2006bFu et al 2003;Bai et al 2006;Zhou et al 2006;Tan et al 2006), except one case in which N. cincticeps actually performed better on Bt rice KMD1 and KMD2 under laboratory and Þeld conditions (Zhou et al 2005). However, the impact of Bt rice on nontarget organisms was evaluated either in the laboratory or in the Þeld with transplants.…”

Section: Abstract Bt Rice Nontarget Insect Rice Thrip Stenchaetotmentioning

confidence: 99%

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Impacts of SixBtRice Lines on Nontarget Rice Feeding Thrips Under Laboratory and Field Conditions

Akhtar¹,

Tian²,

Chen³

et al. 2010

Environ Entomol

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Nontarget impacts of six transgenic Bt rice lines (expressing the Cry1Ab or Cry1Ab/ Cry1Ac protein) on the thrips, Stenchaetothrips biformis (Bagnall), attacking the rice seedling and tillering stages, were evaluated under laboratory and Þeld conditions. Laboratory results showed relatively longer larval, pupal development and preoviposition durations of S. biformis. Although it had a shorter oviposition period, female adult longevity and less total laid eggs were found when fed on some tested Bt rice in comparison to non-Bt controls. S. biformis population dynamics in Bt and non-Bt plots were monitored using the plastic bag and beat plate methods. In the Þeld, the temporal patterns of S. biformis population changes were similar between tested Bt rice lines and their respective control; however, the total number of S. biformis individuals collected from the Bt plots were signiÞcantly less or the same, varying from variety to variety, compared with those from the non-Bt plots. ELISA results showed that the Bt insecticidal protein could be transferred from Bt rice to the thrips, and the concentrations of the protein in rice leaves and thrips were not signiÞcantly correlated with some important biological parameters of the thrip. In addition, the potential effects of Bt rice on the abundance of S. biformis candidate predators are also discussed. In conclusion, our results show that the six Bt rice lines assessed may be less preferable host plants to S. biformis at the individual and population levels in comparison to the non-Bt rice plants.

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Section: Discussionmentioning

confidence: 85%

Section: Abstract Bt Rice Nontarget Insect Rice Thrip Stenchaetotmentioning

confidence: 99%

Section: Abstract Bt Rice Nontarget Insect Rice Thrip Stenchaetotmentioning

confidence: 99%

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Impacts of SixBtRice Lines on Nontarget Rice Feeding Thrips Under Laboratory and Field Conditions

Akhtar¹,

Tian²,

Chen³

et al. 2010

Environ Entomol

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“…in Bt cotton (Head et al, 2005). In addition, the deployment of IRGM plants can reduce the insecticide-induced resurgence of secondary pests, as reported from aphids in Bt cotton and planthoppers in Bt rice (Chen et al, 2006). However, secondary pest outbreaks also have been reported from Bt crops.…”

Section: Non-target Effects Caused By Changes In Agricultural Practicementioning

confidence: 99%

“…For example, cry1Ab and cry1Ac genes have been fused to produce 'hybrid' Bt cotton (Yao et al, 2006). Since each Bt toxin in this case is known to be safe for honeybees, combining them in this way is unlikely to present a new hazard to these and other non-lepidopteran pollinators.…”

Section: Gm Plants Expressing Btmentioning

confidence: 99%

Environmental Impact of Genetically Modified Crops

Ferry¹,

Gatehouse²

2009

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This book, containing 20 chapters, addresses the major concerns of scientists, policy makers, environmental lobby groups and the general public regarding the controversial issue on environmental impact (e.g. on soil and water ecology and nontarget organisms) of transgenic crops, from an editorially neutral standpoint. While the main focus is on environmental impact, food safety issues for both humans and animals are also considered. Some chapters discuss issues on resistance management. The book concludes with a discussion on the future of agricultural biotechnology in the context of sustainability, natural resource management and future global population and food supply.

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Marker‐free, tissue‐specific expression of Cry1Ab as a safe transgenic strategy for insect resistance in rice plants

Chen

et al. 2012

Pest Management Science

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BACKGROUND: Rice is the major food resource for nearly half of the global population; however, insect infestation could severely affect the production of this staple food. To improve rice insect resistance and reduce the levels of Bt toxin released into the environment, the Cry1Ab gene was conjugated to the rice rbcS promoter to express Bt toxin in specific tissues of transgenic plants. RESULTS: Eight marker‐free, T2 lines were separated from the T0 cotransformants. Using RT‐PCR, high levels of Cry1Ab expression were detected in the leaf but not in the seed. The Cry1Ab protein level ranged from 1.66 to 3.31 µg g−1 in the leaves of four transgenic lines, but was barely detectable in their seeds by ELISA. Bioassays showed that the mortality rate of silkworm larvae feeding on mulberry leaves dipped in transgenic rice flour and pollen was less than that of the positive control (KMD), and that their average weight was higher than that of KMD, suggesting that the Cry1Ab protein was not expressed in the seed and pollen. CONCLUSION: The transgene conferred a high level of resistance to insects and biosafety to the rice plants, which could be directly used in rice breeding. Copyright © 2012 Society of Chemical Industry

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Field Assessment of the Effects of Transgenic Rice Expressing a Fused Gene ofcry1Abandcry1AcfromBacillus thuringiensisBerliner on Nontarget Planthopper and Leafhopper Populations

Cited by 57 publications

References 25 publications

Impacts of SixBtRice Lines on Nontarget Rice Feeding Thrips Under Laboratory and Field Conditions

Impacts of SixBtRice Lines on Nontarget Rice Feeding Thrips Under Laboratory and Field Conditions

Environmental Impact of Genetically Modified Crops

Marker‐free, tissue‐specific expression of Cry1Ab as a safe transgenic strategy for insect resistance in rice plants

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