Field Competitiveness of Double Translocation Heterozygote Males of Aedes Aegypti (L.)

Seawright, J. A.; Kaiser, P. E.; Willis, N. L.; Dame, David A.

doi:10.1093/jmedent/13.2.208

Cited by 13 publications

(5 citation statements)

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“…We wish to distinguish between the genetic fitness cost imposed by the toxins of the α and β alleles and changes in life history parameters that could be caused by lab rearing, loss of 'wild' phenotypic behaviours and any manifestation of genetic load caused by the UD constructs that are not directly associated with heterozygote lethality or ambient genetic transgene fitness cost [55][56][57]. To this end, we consider the effects of an increased scale of larval competition (ν), slower maturation (m), a higher rate of adult (µ) and larval (µ B ) density-independent mortality and a greater strength of density dependence (η).…”

Section: Ecological Fitness Costsmentioning

confidence: 99%

Ecological effects on underdominance threshold drives for vector control

Khamis

Mouden

Kura

et al. 2018

Journal of Theoretical Biology

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Underdominance gene drives are frequency-dependent drives that aim to spread a desired homozygote genotype within a population. When the desired homozygote is released above a threshold frequency, heterozygote fitness disadvantage acts to drive the desired trait to fixation. Underdominance drives have been proposed as a way to control vector-borne disease through population suppression and replacement in a spatially contained and reversible way-benefits that directly address potential safety concerns with gene drives. Here, ecological and epidemiological dynamics are coupled to a model of mosquito genetics to investigate theoretically the impact of different types of underdominance gene drive on disease prevalence. We model systems with two engineered alleles carried either on the same pair of chromosomes at the same locus or homozygously on different pairs at different loci, genetic lethality that affects both sexes or only females, and bi-sex or male-only releases. Further, the different genetic and ecological fitness costs that can arise from genetic modification and artificial rearing are investigated through their effect on the population threshold frequency that is required to trigger the drive mechanism. We show that male-only releases must be significantly larger than bi-sex releases to trigger the underdominance drive. In addition, we find that female-specific lethality averts a higher percentage of disease cases over a control period than does bi-sex lethality. Decreases in the genetic fitness of the engineered homozygotes can increase the underdominance threshold substantially, but we find that the mating success of transgenic mosquitoes with wild-type females (influenced by a lack of competitiveness or the evolution of behavioural resistance in the form of active female mate preference) and the longevity of artificially-reared mosquitoes are vitally important to the success chances of underdominance based gene drive control efforts.

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Section: Ecological Fitness Costsmentioning

confidence: 99%

Ecological effects on underdominance threshold drives for vector control

Khamis

Mouden

Kura

et al. 2018

Journal of Theoretical Biology

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“…Such potential reduction in performance must however be confirmed in the field as laboratory-based and field-based trials do not always show similar effects [47], [48], [49]. Furthermore, the unmodified line used in these experiments has been reared under laboratory conditions for many generations.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Life History Characteristics of the Genetically Modified OX513A Line and a Wild Type Strain of Aedes aegypti

et al. 2011

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The idea of implementing genetics-based insect control strategies modelled on the traditional SIT (Sterile Insect Technique), such as RIDL (Release of Insects carrying a Dominant Lethal), is becoming increasingly popular. In this paper, we compare a genetically modified line of Aedes aegypti carrying a tetracycline repressible, lethal positive feedback system (OX513A) with a genetically similar, unmodified counterpart and their respective responses to increasing larval rearing density using a constant amount of food per larva. The parameters that we examined were larval mortality, developmental rate (i.e., time to pupation), adult size and longevity.Analysis revealed some statistically significant differences between the life history traits we examined. The genetically modified OX513A line overall showed 5% lower larval survival as well as reduced adult longevity (20 vs 24 days mean lifespan) compared to the unmodified line. Furthermore, the OX513A line pupated about one day sooner, which could be advantageous in mass-rearing, but produced somewhat smaller adults than the unmodified line; this effect was more pronounced in females than in males.Increasing the larval rearing density delayed pupation, decreased adult longevity and reduced adult size in both lines. While the delay in pupation and the decrease in longevity were similar between the two lines, the decrease in adult size was more pronounced for OX513A males.Our study shows that in a controlled laboratory situation the transgenic sterile OX513A line may have somewhat reduced performance compared to its unmodified counterpart and that high rearing densities may further reduce performance. Laboratory-based cage trials as well as field trials are necessary to assess how the differences in life history traits documented here impact the males' success upon release. Furthermore, this paper highlights the potential value of optimisation of mass-rearing systems as optimised rearing methods may be able to alleviate performance issues associated with specific lines or with lab-adapted lines in general.

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“…Their results indicated control of hypothetical insect populations by releases of translocation heterozygotes if the reproductive rate was 5-fold or less per generation. In mating competition experiments, translocation stocks have been shown to foe competitive with wild-type strains (Rai et al, 1970;Seawright et al, 1915b;Seawright et al, 1976), although Grover et al (1976) reported reduced competitiveness with one of their double translocation heterozygote stocks. Curtis et al (1976) have demonstrated the inheritance of sterility after the termination of releases of double translocation heterozygotes in laboratory cages.…”

Section: Introductionmentioning

confidence: 99%

Field trials of double translocation heterozygote males for genetic control ofAedes aegypti(L.) (Diptera: Culicidae)

Petersen

Lounibos

Lorimer

1977

Bull. Entomol. Res.

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For nine weeks from 14 April to 13 June 1975 an average of over 500 double translocation heterozygote male Aedes aegypti (L.) were released daily into a village where the estimated adult mosquito population was 1200. The two purposes of this release were to suppress the A. aegypti population by sterility induced by chromosomal translocations and to assess the effect of rainy season conditions on the population dynamics of the indigenous and released mosquitoes. As a result of the releases, hatcha'bility of eggs from females collected during landing-biting catches dropped from over 93% to 30-40%. For eight weeks following the termination of the releases, hatchability remained below 70 % while that of the reference village averaged between 80 and 99 % hatch. A relative population decline observed in the release village is not attributed solely to the effect of the releases, partly because it occurred before sufficient time had elapsed to allow induced sterility to build up in the field population.

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Field Competitiveness of Double Translocation Heterozygote Males of Aedes Aegypti (L.)

Cited by 13 publications

References 0 publications

Ecological effects on underdominance threshold drives for vector control

Ecological effects on underdominance threshold drives for vector control

Comparison of Life History Characteristics of the Genetically Modified OX513A Line and a Wild Type Strain of Aedes aegypti

Field trials of double translocation heterozygote males for genetic control ofAedes aegypti(L.) (Diptera: Culicidae)

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