Field evaluation of the efficacy, compatibility and serologic profiling of a combined vaccine against porcine reproductive and respiratory syndrome and Haemophilus parasuis in nursery pigs

Palzer, Andreas; Eddicks, Matthias; Zoels, Susanne; Stark, Jasmin; Reese, Sven; Strutzberg-Minder, Katrin; Fiebig, Kerstin; Ritzmann, M.

doi:10.1016/j.prevetmed.2015.03.005

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…Haemophilus parasuis can cause severe system inflammation alone or induce secondary infection after porcine reproductive and respiratory syndrome virus, pseudorabies virus, porcine circovirus, or another virus infection in the infected pigs (28)(29)(30). Haemophilus parasuis infection is increasingly harmful to the global swine industry, and the prevention and control of H. parasuis have always been a significant challenge, as the pathogenicity of H. parasuis is not fully characterized.…”

Section: Discussionmentioning

confidence: 99%

Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis

Zhang¹,

Chu²,

Song³

et al. 2021

Front. Vet. Sci.

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Haemophilus parasuis has emerged as an important bacterial pathogen in pig husbandry, as H. parasuis can coinfect pigs with a variety of pathogenic microorganisms and further cause an aggravation of the disease. It is crucial to investigate its pathogenetic mechanism. Gram-negative bacteria naturally secrete outer membrane vesicles (OMVs), and their potent virulence factors play prominent roles that affect the interaction between bacteria and host. Still, the pathogenesis that is associated with the bacterial OMVs has not been well-elucidated. In this study, we investigated the secretion of OMVs from a clinical H. parasuis isolate strain (H45). In addition, we further analyzed the characterization, the comprehensive proteome, and the virulence potential of OMVs. Our data demonstrated that H. parasuis could secrete OMVs into the extracellular milieu during infection. Using liquid chromatography with tandem mass spectrometry (MS/MS) identification and bio-information analysis, we identified 588 different proteins associated with OMVs. Also, we also analyzed the subcellular location and biological function of those proteins. These proteins are mainly involved in immune and iron metabolism. Moreover, we confirmed the pathogenicity of H. parasuis OMVs by observing a strong inflammatory response in J774A.1 and porcine alveolar macrophages. Taken together, our findings suggested that OMVs from H. parasuis were involved in the pathogenesis of this bacterium during infection.

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Section: Discussionmentioning

confidence: 99%

Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis

Zhang¹,

Chu²,

Song³

et al. 2021

Front. Vet. Sci.

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“…Such outbreaks can cause 100% morbidity and >40% mortality in infected piglets [ 11 , 12 ]. In PRRSV-infected animals, the respiratory tract becomes more prone to other pathogens, such as PCV2 [ 13 , 14 , 15 ], Classical swine fever virus (CSFV), SS, Gps, Mh, and various Salmonella species (spp), which may affect the severity of PRRSV-induced pathogenesis [ 16 , 17 , 18 , 19 , 20 , 21 ]. Previous studies on bacterial ecology in Chinese pig farms identified SS (77.92%), Gps (51.25%), Escherichia coli (52.39%), and Pasteurella multicide (26.77%) infections, along with the endemic of PRRSV, under field conditions [ 1 , 20 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Secondary Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2) Infection Augments Inflammatory Responses, Clinical Outcomes, and Pathogen Load in Glaesserella-parasuis-Infected Piglets

Guan

Pang

Ouyang

et al. 2023

Veterinary Sciences

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Glaesserella parasuis (Gps), Gram-negative bacteria, are a universal respiratory-disease-causing pathogen in swine that colonize the upper respiratory tract. Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2HP-PRRSV2) and Gps coinfections are epidemics in China, but little is known about the influence of concurrent coinfection on disease severity and inflammatory responses. Herein, we studied the effects of secondary HP-PRRS infection on clinical symptoms, pathological changes, pathogen load, and inflammatory response of Gps coinfection in the upper respiratory tract of piglets. All coinfected piglets (HP-PRRSV2 + Gps) displayed fever and severe lesions in the lungs, while fever was present in only a few animals with a single infection (HP-PRRSV2 or Gps). Additionally, HP-PRRSV2 and Gps loading in nasal swabs and blood and lung tissue samples was significantly increased in the coinfected group. Necropsy data showed that coinfected piglets suffered from severe lung damage and had significantly higher antibody titers of HP-PRRSV2 or Gps than single-infected piglets. Moreover, the serum and lung concentrations of inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-8) were also significantly higher in coinfected piglets than in those infected with HP-PRRSV2 or Gps alone. In conclusion, our results show that HP-PRRSV2 promotes the shedding and replication of Gps, and their coinfection in the upper respiratory tract aggravates the clinical symptoms and inflammatory responses, causing lung damage. Therefore, in the unavoidable situation of Gps infection in piglets, necessary measures must be made to prevent and control secondary infection with HP-PRRSV2, which can save huge economic losses to the pork industry.

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“…In recent years, under the condition of farming cluster culture, due to the expansion of breeding scale and density, Glässer's disease has remained a significant economic burden for the swine industry. It causes serious damage and death to pigs through coinfection or secondary infection with other pathogens, such as porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, swine influenza A virus, and mycoplasma hyopneumoniae [4][5][6][7]. The high mortality and morbidity caused by the coinfection or secondary infection of G. parasuis pose a great challenge to the prevention and control of this disease.…”

Section: Introductionmentioning

confidence: 99%

Rapid Visual Detection of Glaesserella parasuis with a Real-Time Recombinase-Aided Amplification Assay

Kang,

Chen,

Yang

et al. 2023

Transboundary and Emerging Diseases

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Glaesserella parasuis is a specific bacterial pathogen of Glässer’s disease which causes significant economic losses to the swine industry. Dependable and rapid detection of G. parasuis is crucial to prevent and control Glässer’s disease outbreaks. In this study, a recombinase-aided amplification (RAA) assay based on the infB gene was developed to rapid detect G. parasuis. The novel method performs isothermal detection at 42°C for real-time analysis or visualization and data analysis (RAA-VDA). The developed assay showed high specificity for G. parasuis detection without cross-reactions to other clinically important swine pathogens. The analytical sensitivity of real-time RAA was 67.17 copies per reaction with 95% reliability, which was comparable to the G. parasuis quantitative real-time PCR (qPCR). However, the detection limit of RAA-VDA was 142.43 copies per reaction with 95% reliability. The coefficient of variation analysis of the intrabatch and interbatch experimental replicate results were less than 4.30% and 6.74%, respectively, indicating the real-time RAA assay had high repeatability and reproducibility. A total of 108 clinical tissue samples were used to evaluate the clinical diagnostic performance. The diagnostic accordance rates of qPCR with real-time RAA and RAA-VDA were 100% and 98.15% (106/108), respectively. This system combined instrumental analysis and visualized analysis to accomplish a new try for rapid detection of G. parasuis in clinical practice.

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Field evaluation of the efficacy, compatibility and serologic profiling of a combined vaccine against porcine reproductive and respiratory syndrome and Haemophilus parasuis in nursery pigs

Cited by 6 publications

References 25 publications

Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis

Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain of Haemophilus parasuis

Secondary Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2) Infection Augments Inflammatory Responses, Clinical Outcomes, and Pathogen Load in Glaesserella-parasuis-Infected Piglets

Rapid Visual Detection of Glaesserella parasuis with a Real-Time Recombinase-Aided Amplification Assay

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