Field-Flow Fractionation

Caldwell, Karin D.; Wahlund, Karl-Gustav

doi:10.1021/ac00168a717

Cited by 54 publications

(2 citation statements)

References 16 publications

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“…This report describes the use of CE for rapid, sensitive resolution of polystyrene nanospheres ranging from 39 to 683 nm in diameter. These separations appear to be based upon capillary wall-particle interactions iatki than predicted electrophoretic mechanisms; yet resolution is superior to that observed using other elution techniques (11)(12)(13).…”

Section: Introductionmentioning

confidence: 86%

Analytical separation of polystyrene nanospheres by capillary electrophoresis

VanOman

McIntire

1989

J Microcolumn Separations

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Abstract. The analytical capillary electrophoretic separation of polystyrene nanospheres is reported. Although these particles are negatively charged and possess intrinsic mobilities, their separations are based upon particle size with the smallest particles having the highest velocity through the capillary. Pretreatment of the capillary with a cationic surfactant is necessary to observe these separations. Interestingly, particles larger than about 700 nm in diameter cannot be observed to move through a 50-pmi.d. capillary, whereas particles as small as 39 nm in diameter can be resolved in this system. In contrast to normal electrophoretic separations, the mechanism of this process appears to be that of particlexapillary wall interaction as mediated by the adsorbed surfactant.

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Section: Introductionmentioning

confidence: 86%

Analytical separation of polystyrene nanospheres by capillary electrophoresis

VanOman

McIntire

1989

J Microcolumn Separations

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“…Field flow fractionation (FFF) separates molecules by applying a perpendicular parabolic flow to the sample in a narrow channel. There are four main configurations of fields in FFF-sedimentation, thermal, hydraulic (or flow), and electrical-where the "field" describes the environment in which the particles are separated (Caldwell, 1988). For the separation of whey protein, in native or soluble aggregate form, hydraulic (sometimes known as flow, or asymmetric flow) separation is most applicable.…”

Section: Field Flow Fractionationmentioning

confidence: 99%

From lab‐based to in‐line: Analytical tools for the characterization of whey protein denaturation and aggregation–A review

Finnegan,

Goulding,

O'Callaghan

et al. 2024

Comp Rev Food Sci Food Safe

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Whey protein denaturation and aggregation have long been areas of research interest to the dairy industry, having significant implications for process performance and final product functionality and quality. As such, a significant number of analytical techniques have been developed or adapted to assess and characterize levels of whey protein denaturation and aggregation, to either maximize processing efficiency or create products with enhanced functionality (both technological and biological). This review aims to collate and critique these approaches based on their analytical principles and outline their application for the assessment of denaturation and aggregation. This review also provides insights into recent developments in process analytical technologies relating to whey protein denaturation and aggregation, whereby some of the analytical methods have been adapted to enable measurements in‐line. Developments in this area will enable more live, in‐process data to be generated, which will subsequently allow more adaptive processing, enabling improved product quality and processing efficiency. Along with the applicability of these techniques for the assessment of whey protein denaturation and aggregation, limitations are also presented to help assess the suitability of each analytical technique for specific areas of interest.

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Separation of protein inclusion bodies fromEscherichia coli lysates using sedimentation field-flow fractionation

et al. 1997

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I am very fortunate to have had the opportunity to work closely with Professor J. Calvin Giddings for the past 10 years. During this time, he has been both a mentor and a friend. The guidance and freedom that he has given me over the years have greatly impacted my professional growth and research directions. His insightful approaches to scientific problems, his thoroughness, and his good naturedness during times of stress are lessons that will always be remembered.Cal Giddings approached recreational activities with the same zeal as he did work. Our skiing, river running, and mountain biking expeditions were both enjoyable and intense. The path that Cal inevitably took was the one where all others had failed or avoided because of its difficulty. Just as with work, there were no obstacles that were unsurmountable. They were challenges that could be overcome. Over the years, this has also become my personal philosophy.I will miss him a great deal.

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Field-Flow Fractionation

Cited by 54 publications

References 16 publications

Analytical separation of polystyrene nanospheres by capillary electrophoresis

Analytical separation of polystyrene nanospheres by capillary electrophoresis

From lab‐based to in‐line: Analytical tools for the characterization of whey protein denaturation and aggregation–A review

Separation of protein inclusion bodies fromEscherichia coli lysates using sedimentation field-flow fractionation

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