Separation of protein inclusion bodies fromEscherichia coli lysates using sedimentation field-flow fractionation

Abstract

I am very fortunate to have had the opportunity to work closely with Professor J. Calvin Giddings for the past 10 years. During this time, he has been both a mentor and a friend. The guidance and freedom that he has given me over the years have greatly impacted my professional growth and research directions. His insightful approaches to scientific problems, his thoroughness, and his good naturedness during times of stress are lessons that will always be remembered.Cal Giddings approached recreational activities with the same zeal as he did work. Our skiing, river running, and mountain biking expeditions were both enjoyable and intense. The path that Cal inevitably took was the one where all others had failed or avoided because of its difficulty. Just as with work, there were no obstacles that were unsurmountable. They were challenges that could be overcome. Over the years, this has also become my personal philosophy.I will miss him a great deal.