Field-flow fractionation of nano- and microparticles in rotating coiled columns

Fedotov, Petr S.; Ermolin, Mikhail S.; Katasonova, O. N.

doi:10.1016/j.chroma.2014.12.079

Cited by 26 publications

(15 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…sdFFF can effectively separate particles based on size, volume, mass and density with respect to the mobile phase [9]. It has the power and resolution to separate particles from 1nm to ~50μm in size, and effectively separated nano and microparticles such as dust, silica beads, sand and soil particles, achieved by simply changing the flow rate [10], offering an intriguing opportunity for virus and phage separation.…”

Section: Countercurrent Chromatography and Field-flow Fractionationmentioning

confidence: 99%

“…No areas of lysis or plaques were seen for fraction number 26 on the dilution gradient, but given the area of lysis present on the initial spot test for this fraction (data not shown), this would suggest a concentration of phages in the fraction of less than 10 5 PFU/ml. The concentration gradient revealed that the initial concentration of the phage solution was ~10 10 , when calculating for dilutions. As with the T4 samples, the areas of lysis were indeed caused by phage, shown by observing areas of lysis in samples propagated from positive spots.…”

Section: ϕX174mentioning

confidence: 99%

See 1 more Smart Citation

The Application of Countercurrent Chromatography for the study of Bacteriophages

Friedersdorff

Bright²,

Rooke³

et al. 2021

Preprint

View full text Add to dashboard Cite

1.AbstractBacteriophages (phages) are viruses that target bacteria, with the ability to lyse and kill host bacterial cells. Due to this, they have been of some interest as a therapeutic since their discovery in the early 1900s, but with the recent increase in antibiotic resistance, phages have seen a resurgence in attention. Current methods of isolation and purification of phages can be long and tedious, with caesium chloride concentration gradients the gold standard for purifying a phage fraction. Isolation of novel phages requires centrifugation and ultrafiltration of mixed samples, such as water sources, effluent or faecal samples etc, to prepare phage filtrates for further testing. We propose countercurrent chromatography as a novel and alternative approach to use when studying phages, as a scalable and high-yield method for obtaining phage fractions. However, the full extent of the usefulness and resolution of separation with this technique has not been researched; it requires optimisation and ample testing before this can be revealed. Here we present an initial study to determine survivability of two phages, T4 and ϕX174, using only water as a mobile phase in a Spectrum Series 20 HPCCC. Both phages were found to remain active once eluted from the column. Phages do not fully elute from the column and sodium hydroxide is necessary to flush the column between runs to deactivate remaining phages.

show abstract

Section: Countercurrent Chromatography and Field-flow Fractionationmentioning

confidence: 99%

Section: ϕX174mentioning

confidence: 99%

The Application of Countercurrent Chromatography for the study of Bacteriophages

Friedersdorff

Bright²,

Rooke³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…131 In FFF sample fractionation is performed inside a trapezoidal channel without a stationary phase and is the only technique that offers a continuous, high size-resolution fractionation from about 1 nm up to several µm. 39 Briefly, most of the FFF subtechniques comprise channel dimensions of 20-50 cm length, 2-3 cm width and 0.01-0.05 cm in thickness; 132 (alternatively, and mentioned for the sake of completeness, FFF in rotating coiled columns has also been described in few studies 133,134,135,136 ). A pumped carrier liquid is moving the analytes/particles from the "inlet side" to the "outlet/detector side" generating a parabolic flow profile.…”

Section: Field-flow Fractionation (Fff) -General Concept and Theorymentioning

confidence: 99%

ICP-MS for the analysis at the nanoscale – a tutorial review

Meermann

Nischwitz

2018

J. Anal. At. Spectrom.

187

105

View full text Add to dashboard Cite

The scope of this tutorial review is (i) to provide an overview on ICP-MS based techniques for the analysis of ENPs and natural nanoparticles/colloids by (a) “stand alone” ICP-MS and (b) hyphenated techniques; (ii) highlighting the benefits and pitfalls of each technique as well as providing practical advice regarding method development; (iii) illustrating the possibilities and limitations of each technique by practical applications from the recent literature.

show abstract

“…Обычно скорость вращения центрифуги составляет около 2 500 об / мин, однако, для увеличения разрешения между фракциями частиц разных размеров и расширения их диапазона можно воспользоваться предусмотренным градиентным уменьшением скорости вращения. Метод достаточно удобен и даже позволяет нарабатывать небольшие количества отдельных фракций для их дальнейшего анализа лабораторными методами, что полезно при отработке технологий создания определенных наночастиц или при исследованиях сложных природных объектов, например, илистых отложений или образцов, отобранных из окру жающего воздуха [12]. Для биомолекул -это в первую очередь метод исследования агрегации, так как центробежные силы, равно как и асимметричные потоки, не оказывают никакого действия на самые неустойчивые агрегаты [13].…”

Section: том 9 № 1 2019unclassified

Системы Проточного Фракционирования Макромолекул И Наночастиц В Поперечных Полях

Lermontov¹,

Родченкова²

2019

AnalyticsRussia

View full text Add to dashboard Cite

Описан принцип действия аналитических систем на основе проточного фракционирования частиц в поперечных полях различной природы. Представлена история метода, механизмы проточного фракционирования в асимметричных потоках, электрическом и гравитационном полях, под действием центробежных сил, а также в условиях градиента температуры. Обсуждаются особенности и возможности, области применения и объекты исследования каждой модификации метода. Описано комплексное решение компании Postnova Analytics – платформа FFF (Field-Flow Fractionation) для проточного фракционирования частиц в поперечных полях, в которой интегрированы модули для разделения, детекторы, системы для стабилизации температуры и другие компоненты под единым программным управлением.

show abstract

Field-flow fractionation of nano- and microparticles in rotating coiled columns

Cited by 26 publications

References 19 publications

The Application of Countercurrent Chromatography for the study of Bacteriophages

The Application of Countercurrent Chromatography for the study of Bacteriophages

ICP-MS for the analysis at the nanoscale – a tutorial review

Системы Проточного Фракционирования Макромолекул И Наночастиц В Поперечных Полях

Contact Info

Product

Resources

About