Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis δ-endotoxin

Tu, J. C.; Zhang, Guoan; Datta, Karabi; Xu, Caiguo; He, Yuqing; Zhang, Qifa; Khush, G. S.; Datta, Swapan K.

doi:10.1038/80310

Cited by 411 publications

(274 citation statements)

References 21 publications

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“…The most commonly used Bt genes in transgenic crops including rice are Cry1Ab, Cry1Ac, and a fusion gene of Cry1Ac/Cry1Ab or Cry1Ab/c (19)(20)(21)(22)(23)(24)(25)(26)(27)(28). However, binding tests of midgut brush border membrane vesicles showed that Cry1Aa, Cry1Ab, and Cry1Ac toxins share a common binding site (29)(30)(31); thus, a mutant that is able to overcome one of the Cry1A genes is also likely to be resistant to other Cry1A genes as well.…”

Section: Progresses In Gene Identification and Development Of Gsrmentioning

confidence: 99%

“…2 A and B), the restorer line for a number of elite rice hybrids widely cultivated in China (28). Results from large-scale field tests in several provinces of China showed that this line and its hybrid with Zhenshan 97A, the male sterile line of the most popular hybrid Shanyou 63, were highly resistant to leaffolders and stemborers under field conditions, thus promising to significantly reduce insecticides, labor, and related costs while at the same time increase the yield and benefit the health of the farmers and consumers (33).…”

Section: Progresses In Gene Identification and Development Of Gsrmentioning

confidence: 99%

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Strategies for developing Green Super Rice

Zhang

2007

Proc. Natl. Acad. Sci. U.S.A.

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This contribution is part of the special series of Inaugural Articles by members of the National Academy of Sciences elected on May 1, 2007.From a global viewpoint, a number of challenges need to be met for sustainable rice production: (i) increasingly severe occurrence of insects and diseases and indiscriminate pesticide applications; (ii) high pressure for yield increase and overuse of fertilizers; (iii) water shortage and increasingly frequent occurrence of drought; and (iv) extensive cultivation in marginal lands. A combination of approaches based on the recent advances in genomic research has been formulated to address these challenges, with the long-term goal to develop rice cultivars referred to as Green Super Rice. On the premise of continued yield increase and quality improvement, Green Super Rice should possess resistances to multiple insects and diseases, high nutrient efficiency, and drought resistance, promising to greatly reduce the consumption of pesticides, chemical fertilizers, and water. Large efforts have been focused on identifying germplasms and discovering genes for resistance to diseases and insects, N-and P-use efficiency, drought resistance, grain quality, and yield. The approaches adopted include screening of germplasm collections and mutant libraries, gene discovery and identification, microarray analysis of differentially regulated genes under stressed conditions, and functional test of candidate genes by transgenic analysis. Genes for almost all of the traits have now been isolated in a global perspective and are gradually incorporated into genetic backgrounds of elite cultivars by molecular marker-assisted selection or transformation. It is anticipated that such strategies and efforts would eventually lead to the development of Green Super Rice.genomics ͉ rice genetic improvement ͉ sustainable agriculture

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Section: Progresses In Gene Identification and Development Of Gsrmentioning

confidence: 99%

Section: Progresses In Gene Identification and Development Of Gsrmentioning

confidence: 99%

Strategies for developing Green Super Rice

Zhang

2007

Proc. Natl. Acad. Sci. U.S.A.

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728

438

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“…All the marker-free Ds(Bt) plants expressed the Bt protein at levels comparable with the positive control T51-1, a previously characterized Bt transgenic rice line (Tu et al, 2000).…”

Section: Analysis Of Transposition Activity In T1 Plantsmentioning

confidence: 99%

Efficient Generation of Marker-Free Transgenic Rice Plants Using an Improved Transposon-Mediated Transgene Reintegration Strategy

Gào

Zhou

et al. 2014

Plant Physiol.

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(S.Q.).Marker-free transgenic plants can be developed through transposon-mediated transgene reintegration, which allows intact transgene insertion with defined boundaries and requires only a few primary transformants. In this study, we improved the selection strategy and validated that the maize (Zea mays) Activator/Dissociation (Ds) transposable element can be routinely used to generate marker-free transgenic plants. A Ds-based gene of interest was linked to green fluorescent protein in transfer DNA (T-DNA), and a green fluorescent protein-aided counterselection against T-DNA was used together with polymerase chain reaction (PCR)-based positive selection for the gene of interest to screen marker-free progeny. To test the efficacy of this strategy, we cloned the Bacillus thuringiensis (Bt) d-endotoxin gene into the Ds elements and transformed transposon vectors into rice (Oryza sativa) cultivars via Agrobacterium tumefaciens. PCR assays of the transposon empty donor site exhibited transposition in somatic cells in 60.5% to 100% of the rice transformants. Marker-free (T-DNA-free) transgenic rice plants derived from unlinked germinal transposition were obtained from the T1 generation of 26.1% of the primary transformants. Individual marker-free transgenic rice lines were subjected to thermal asymmetric interlaced-PCR to determine Ds(Bt) reintegration positions, reverse transcription-PCR and enzyme-linked immunosorbent assay to detect Bt expression levels, and bioassays to confirm resistance against the striped stem borer Chilo suppressalis. Overall, we efficiently generated marker-free transgenic plants with optimized transgene insertion and expression. The transposon-mediated marker-free platform established in this study can be used in rice and possibly in other important crops.

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“…The use of genes encoding insecticidal proteins from Bacillus thuringiensis to confer resistance to various insect pests has been very successful in a number of crops (Moellenbeck et al ., 2001;Perlak et al ., 2001;Tu et al ., 2000). More recently, chitinase-encoding transgenes from certain fungi have been shown to improve plant defence against a broad range of fungal pathogens.…”

Section: Introductionmentioning

confidence: 99%

Enhanced fungal resistance in transgenic cotton expressing an endochitinase gene from Trichoderma virens

Emani

García

Lopata-Finch

et al. 2003

Plant Biotechnology Journal

136

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SummaryMycoparasitic fungi are proving to be rich sources of antifungal genes that can be utilized to genetically engineer important crops for resistance against fungal pathogens. Homozygous T 2 plants of the high endochitinase-expressing cotton lines were tested for disease resistance against a soil-borne pathogen, Rhizoctonia solani and a foliar pathogen, Alternaria alternata . Transgenic cotton plants showed significant resistance to both pathogens.

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Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis δ-endotoxin

Cited by 411 publications

References 21 publications

Strategies for developing Green Super Rice

Strategies for developing Green Super Rice

Efficient Generation of Marker-Free Transgenic Rice Plants Using an Improved Transposon-Mediated Transgene Reintegration Strategy

Enhanced fungal resistance in transgenic cotton expressing an endochitinase gene from Trichoderma virens

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