Field Sanitation and Foliar Application of Streptomyces padanus PMS-702 for the Control of Rice Sheath Blight

Abstract

Rice sheath blight (ShB), caused by Rhizoctonia solani Kühn AG1-IA, is one of the destructive rice diseases worldwide. The aims of this study were to develop biocontrol strategies focusing on field sanitation and foliar application with a biocontrol agent for ShB management. Streptomyces padanus PMS-702 showed a great antagonistic activity against R. solani. Fungichromin produced by S. padanus PMS-702, at 3.07 mg/l inhibited 50% mycelial growth, caused leakage of cytoplasm, and inhibited the formation of infection structures of R. solani. Fungichromin could reach to 802 mg/l when S. padanus PMS-702 was cultured in MACC broth for 6 days. Addition of 0.5% S. padanus PMS-702 broth into soil decreased the survival rate of the pathogen compared to the control. Soil amended with 0.5% S. padanus broth and 0.5% tea seed pomace resulted in the death of R. solani mycelia in the infested rice straws, and the germination of sclerotia was inhibited 21 days after treatment. Greenhouse trials revealed that S. padanus cultured in soybean meal-glucose (SMGC-2) medium after mixing with different surfactants could enhance its efficacy for inhibiting the pathogen. Of six surfactants tested, the addition of 2% tea saponin was the most effective in suppressing the pathogen. S. padanus broth after being fermented in SMGC-2, mixed with 2% tea saponin, diluted 100 fold, and sprayed onto rice plants significantly reduced ShB disease severity. Thus, S. padanus PMS-702 is an effective biocontrol agent. The efficacy of S. padanus PMS-702 for disease control could be improved through formulation.

Isolation, Phenotypic and Molecular Identification of Actinomycetes From Soil and Evaluation of Their Efficiency in Control of the Pathogen Botrytis cinerea Caused Gray Rot Disease on Eggplant

“…WP-1 produces the antibiotic Fungichromin, which has been proven in vitro to have a high ability to inhibit many plant pathogenic fungi such as Fusarium oxysporum, Rhizoctonia solani, Phytophthora infestan. The results of the current study were in agreement with Yang et al [33] that the nutritional medium has an important role in the production of the antibiotic Fungichromin, as the results of the HPLC analysis showed that the concentration of the antibiotic Fungichromin produced by the genus S. padanus increased from 194.34 mg / l to 550.42 mg / l when S. padanus was grown in a nutrient medium consisting of barley and alfalfa seed powder with the addition of 1% corn oil, and when adding 0.10% of calcium carbonate in the medium of barley and alfalfa seed powder and adding 1% corn oil, it enhanced the production of the antibiotic by 26.5 %. The antibiotic Fungichromin is a member of the polyene macrolide group of antibiotics [34].…”

Sheath Blight of Maize: An Overview and Prospects for Future Research Directions

“…The resistant and moderately resistant materials accounted for 3.1% and 13.9%, respectively. Yang [66] conducted field disease resistance tests on 45 maize inbred lines collected from across the country. He found no inbred lines that were immune to sheath blight.…”

“…QTL (quantitative trait locus) mapping reveals the association between genes, loci, and traits, which has been utilized for marker-assisted selection in breeding [8,95,96]. Quantitative trait loci related to resistance have been identified on all 10 pairs of maize chromosomes (Table 3), with a high distribution frequency on chromosomes 1, 2, 4, and 6 [15,66,[97][98][99][100][101][102][103][104][105]. Wisser summarized 437 dQTLs and 17 major disease resistance genes from 50 studies on the genetic structure of maize disease resistance [106].…”

Identification and Characterization of a Multifunctional Biocontrol Agent, Streptomyces griseorubiginosus LJS06, Against Cucumber Anthracnose

“…For example, Palaniyandi et al (2013) reported that S. phaeopurpureus ExPro138 can produce several proteases, inhibiting spore germination and appressorium formation, act as a hyperparasite of Colletotrichum coccode s, and reduce the incidence of tomato anthracnose ( Palaniyandi et al, 2013 ). Shih et al (2003) and Yang et al (2021) reported that the fungichromin produced by S. padanus PMS-702 can be used to control Rhizoctonia damping-off on cabbage, sheath blight on rice, Phytophthora fruit rot on peach, and downy mildew on cabbage ( Shih et al, 2003 ; Yang et al, 2021 ).…”