Field Testing of a Satellite-Containing Attenuated Strain of Cucumber Mosaic Virus for Tomato Protection in Japan

Sayama, H.; Sato, Tomoi; Kominato, M.; Natsuaki, Tomohide; Kaper, J.M.

doi:10.1094/phyto-83-405

Cited by 54 publications

(24 citation statements)

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“…However, not all instances of cross-protection can be explained this way (Gerber & Sarkar, 1989;Zaitlin, 1976). Coat protein-mediated effects cannot explain cross-protection between viroid strains or protection against virus infection provided by most satellite RNAs, with the exception of turnip crinkle virus-satellite RNA interactions (Gallitelli et al, 1991;Manfre & Simon, 2008;Montasser et al, 1991;Niblett et al, 1978;Sayama et al, 1993Sayama et al, , 2001Tien & Wu, 1991).…”

mentioning

confidence: 99%

Effects of dicer-like endoribonucleases 2 and 4 on infection of Arabidopsis thaliana by cucumber mosaic virus and a mutant virus lacking the 2b counter-defence protein gene

Ziebell

Carr

2009

Journal of General Virology

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In tobacco and Nicotiana benthamiana, limited cross-protection against cucumber mosaic virus strain Fny (Fny-CMV) was provided by prior inoculation with a deletion mutant lacking the 2b silencing-suppressor protein gene (Fny-CMVD2b). Cross-protection by Fny-CMVD2b did not result from induction of systemic RNA silencing. We investigated whether protection occurs through induction of localized RNA silencing by using Arabidopsis thaliana plants harbouring mutations in genes encoding the dicer-like (DCL) endoribonucleases 2, 3 and 4 involved in antiviral silencing. In wild-type A. thaliana (Col-0) plants, Fny-CMVD2b was symptomless and cross-protected against Fny-CMV infection. Cross-protection by Fny-CMVD2b against Fny-CMV infection was not abolished in dcl2, dcl3 or dcl4 mutant plants and was strongest in dcl2/4 double mutants, although in these plants and in dcl4 mutants, Fny-CMVD2b replicated to high levels and induced strong symptoms. The results suggest that Fny-CMVD2b/Fny-CMV crossprotection is not completely dependent on RNA silencing and also involves competition between these viruses.

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confidence: 99%

Effects of dicer-like endoribonucleases 2 and 4 on infection of Arabidopsis thaliana by cucumber mosaic virus and a mutant virus lacking the 2b counter-defence protein gene

Ziebell

Carr

2009

Journal of General Virology

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“…Some attenuated strains (Pa18 and C-1421) of Pepper mild mottle virus (PMMoV) have been used in pepper fields (Goto et al 1984;Nagai 1987). Tomato and tobacco diseases by CMV are also controlled by attenuated strains in Japan and China (Sayama et al 1993;Tien and Wu 1991). In some cases, satellite RNA was added to field isolates of CMV to attenuate symptoms (Sayama et al 1993).…”

Section: Selection Isolation and Induction Of Attenuated Virusesmentioning

confidence: 99%

“…Tomato and tobacco diseases by CMV are also controlled by attenuated strains in Japan and China (Sayama et al 1993;Tien and Wu 1991). In some cases, satellite RNA was added to field isolates of CMV to attenuate symptoms (Sayama et al 1993). In Japan, this strategy has been applied not only to tomato but also to cucumber, petunia and Texas bluebell (Eustoma grandiflorum) (Sayama 1996).…”

Section: Selection Isolation and Induction Of Attenuated Virusesmentioning

confidence: 99%

“…Other types of attenuated CMV strains harbor satellite RNA that affects viral pathogenesis. An attenuating satellite RNA has base substitutions and insertions in the region responsible for the necrotic response and an insertion/deletion in other regions (Sayama et al 1993). The role of these base changes in attenuating symptoms remains to be resolved.…”

Section: Cucumovirusmentioning

confidence: 99%

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Attenuated plant viruses: preventing virus diseases and understanding the molecular mechanism

Nishiguchi

Kobayashi

2011

J Gen Plant Pathol

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Attenuated viruses have been isolated and studied not only as a practical means of controlling virus diseases but also to gain a molecular understanding of viral virulence and cross protection. They have been isolated from crop fields and generated through high/low temperature treatment or by mutagens such as nitrous acid and ultraviolet irradiation. Some viruses have been beneficially used in fields and evaluated for one or more decades. Molecular genetic studies on attenuated viruses have revealed that amino acid substitutions are located in replicase and the movement protein in tobamovirus, protein 2b for cucumovirus, and P1 and HC-Pro for potyvirus. In most cases, with a few exceptions, symptom attenuation is positively correlated with a reduced level of RNA silencing suppression. Molecular mechanisms underlying virus attenuation and cross protection and the rationale for practical use of attenuated viruses for effective virus disease control are discussed.

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“…CMV-KU1 has been successfully tested as a protective virus against many lethal CMV viruses such as CMV-D, CMV-F (Montasser et al, 2006b) and CMV-16 (DQ018288.1.). CMV-16, subgroup II, is a Japanese isolate from tomato (Sayama et al, 1993) that contains no viral satellite. It causes severe stunting and fruit malformation in pepper plants.…”

Section: Introductionmentioning

confidence: 99%

Biological Control of a severe viral strain of Cucumber Mosaic Virus(CMV) using a mild strain of CMVassociated with viral satRNA combined with a mixture of plant growth promoting rhizobacteria (PGPRs)

Montasser¹,

Dashti²,

Ali³

et al. 2017

IJBB

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Field Testing of a Satellite-Containing Attenuated Strain of Cucumber Mosaic Virus for Tomato Protection in Japan

Cited by 54 publications

References 0 publications

Effects of dicer-like endoribonucleases 2 and 4 on infection of Arabidopsis thaliana by cucumber mosaic virus and a mutant virus lacking the 2b counter-defence protein gene

Effects of dicer-like endoribonucleases 2 and 4 on infection of Arabidopsis thaliana by cucumber mosaic virus and a mutant virus lacking the 2b counter-defence protein gene

Attenuated plant viruses: preventing virus diseases and understanding the molecular mechanism

Biological Control of a severe viral strain of Cucumber Mosaic Virus(CMV) using a mild strain of CMVassociated with viral satRNA combined with a mixture of plant growth promoting rhizobacteria (PGPRs)

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