Field Trial to Evaluate Vaccine and Antibiotic for Control of Staph. aureus Mastitis in Dairy cattle, Egypt

Ghobrial, Ramy F.; Beskawy, Mohamed A. El; Diasty, Mohamed M. El; Farag, Verginia M.; Eissa, Mohamed

doi:10.5455/ajvs.283165

Cited by 6 publications

(6 citation statements)

References 23 publications

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“…They reported that vaccinated heifers had a 45% reduction in S. aureus intramammary infection compared to the control group [35]. Furthermore, Ghobrial et al [36] investigated that Lysigin was successfully effective in eliminating 20% of S. aureus mastitis in an Egyptian dairy farm. On the other hand, previous results indicate that the vaccine does not entirely protect the udder against S. aureus mastitis [21].…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Lysigin Vaccine in the Prevention of Mastitis in Dairy Cattle

Badr,

Bakry,

Al-Amry

et al. 2023

Veterinary Medical Journal (Giza)

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Mastitis is one of the biggest expenses for the dairy industry which has a substantial impact on dairy cow health. Preventing new infections in cows is the best strategy to manage mastitis. This study aimed to investigate the effect of a commercially available S. aureus bacterin (Lysigin) on minimizing the rate of mastitis in a dairy herd. A total number of 600 Holstein-Friesian dairy cows were involved in the study and received two doses of the vaccine. The enrolled animals were monitored for clinical signs, bacterial culture, somatic cell count (SCC), and costs utilized for treatment during the study. The rate of clinical and subclinical mastitis was reduced from 13% and 29% to 6.7% and 18.3%, respectively after one year following vaccination. Composite milk samples from infected cows were examined for bacteriological isolation of S. aureus and E. coli. S. aureus and E. coli mastitis were determined in percentages of 25% and 30.9%, respectively. Following immunization, S. aureus and E. coli mastitis were decreased to 10% and 23.3%, respectively. The decrease in the rate of E. coli may be related to farm hygiene and environmental management which has a detrimental effect in the control of coliform mastitis. The SCC on milk samples from animals with chronic infection significantly decreased after vaccination. Furthermore, a reduction in bulk milk tank SSC (BTSSC) was detected in the herd during the study. The application of vaccination has a greater impact on reducing the costs utilized for the treatment of mastitis in the herd. These findings indicate that the Lysigin vaccine has a protective effect against S. aureus mastitis and can be utilized as an additional approach for the management of mastitis.

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Section: Discussionmentioning

confidence: 99%

Efficacy of Lysigin Vaccine in the Prevention of Mastitis in Dairy Cattle

Badr,

Bakry,

Al-Amry

et al. 2023

Veterinary Medical Journal (Giza)

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“…They reported that it is a highly immunogenic vaccine. Ghobrial et al (2018) evaluated the efficacy of vaccine and antibiotic for control of mastitis caused by S. aureus in dairy cattle in Egypt. They found that the combination of S. aureus bacterin (Lysigin) with extended antibiotic (Marbocyl 10%) was successfully effective in the elimination of 53.8% of bovine mastitis caused by S. aureus.…”

Section: Vaccination Against Staphylococcus Aureus and Streptococcus mentioning

confidence: 99%

Incidence and Control of Infectious Bacteria in Bovine Milk

Sedky

Ghazy²,

Soliman³

2020

J microb biotech food sci

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Milk is an essential source for the ingredients needed for infant growth and adult health. Healthy milk production is important for consumer safety. The aim of the present review article was to highlight on the incidence and control of the infectious bacteria in bovine milk. Raw bovine milk is considered as a vehicle for many infectious bacterial diseases such as Bovine Tuberculosis, Brucellosis, Leptospirosis and Mycoplasma infection. The main microorganisms which isolated from bovine milk in cows with mastitis were Escherichia coli, Staphylococcus aureus, Streptococcus spp., Streptococcus agalactiae, Pasteurella spp., Klebsiella spp., Pseudomonas spp. and Streptococcus dysgalactiae. Early and accurate diagnosis is necessary for achieving successful control programs against the infectious bacteria in bovine milk. For successful control of the infectious bacterial diseases in bovine milk, it is mandatory to follow preventive measures such as proper hygienic procedures regarding animal husbandry and handling, providing the cows with balanced nutrition, following vaccination programs against bacterial diseases, reporting of the notifiable diseases to the health authorities, meticulous inspection of the consistency and color of milk and pasteurization of milk. Vaccination programs provide protection of dairy farm animals by preventing or reducing bacterial infections which in turn can help safeguard dairy industry. The main treatment protocol of bacterial diseases in dairy cows is by using suitable antibiotics, so it is necessary to carry out drug sensitivity test before choosing the proper antibiotic. However, multidrug resistance (MDR) has become a growing threat regards the management of infectious bacterial diseases. Therefore, the introduction of alternative antimicrobial agents such as herbal treatment, probiotics, nanoparticles, and stem cells therapy is of absolute necessity to guard against bacterial multidrug resistance. Also, animal vaccinations are important tools for protection against many infectious bacterial diseases affecting bovine milk.

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“…Intramammary antibiotic therapies formulated for the treatment of mastitis are generally unsuccessful in eliminating existing S. aureus leading to the culling of the infected animals, but the application of a combination of vaccination and extended antimicrobial treatment can reduce intramammary infectionbyS. aureus [12] Recently, the methicillin-resistant S. aureus (MRSA) isolates have become widely spread all over the world with the risk of resistance to all beta-lactams and other classes of antibiotics. Therefore, the therapeutic choices are limited significantly [13] [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Antibiogram of Staphylococcus aureus in Clinical and Subclinical Mastitis in Holstein Dairy Cows in Egypt

Talaat

Beskawy²,

Atwa³

et al. 2023

Zagazig Veterinary Journal

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Mastitis is a multifactorial and ubiquitous disease that results from interactions between the host, environment, and infectious agents leading to extensive economic losses. The objectives of this study were to estimate the prevalence of S. aureus in clinical and subclinical mastitis in Holstein dairy cows and determine the susceptibility of S. aureus isolatesagainst different antibiotics for screening of antibiotic resistance genes. A total of 415 Holstein dairy cows were randomly selected from three Egyptian governorates. Selected cows were examined for clinical and subclinical mastitis during the period from October 2014 to June 2018. Milk samples were examined for the presence of S. aureus. The S. aureus isolates were subjected to antibiotic sensitivity, molecular identification, and detection of the antibiotic resistance gene.The prevalence of S. aureus isolated from milk samples was 15.4% of which, 14.3% in clinical cases, and 15.7% in subclinical cases.The antibiogram of S. aureus isolates against 13 antibiotics using the disc-diffusion method revealed the highest rate of resistance to Oxacillin (OX) (96.7%), followed by Ampicillin (AM),Cefoxitin (FOX) (93.3%, each), Tetracycline (TE) (73.3%), Cefotaxime (CTX) (70%), Ampicillin/Sulbactam (SAM) (66.7%), Erythromycin (E) and Sulphamethoxazole/Trimethoprim (SXT) (56.7%, each), Gentamicin (GM) (53.3%), Ofloxacin (OFX) (40%), Chloramphenicol (C) (36.7%), Ciprofloxacin (CIP) (30%) and finally Vancomycin (VA) (0%). Molecular PCR assay revealed that all the 16 S. aureus isolates (100%) carried mecA gene, while 15 out of 16 isolates (93.7%) carried blaZ genebut, 8 out of 16 (50%) carried tetK gene, and only one isolate (0.06%) carried fexA gene. Uncontrolled uses of antibiotics in the treatment of mastitis should be restricted and increase awareness about the risk of antimicrobial-resistant bacteria in milk.

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Field Trial to Evaluate Vaccine and Antibiotic for Control of Staph. aureus Mastitis in Dairy cattle, Egypt

Cited by 6 publications

References 23 publications

Efficacy of Lysigin Vaccine in the Prevention of Mastitis in Dairy Cattle

Efficacy of Lysigin Vaccine in the Prevention of Mastitis in Dairy Cattle

Incidence and Control of Infectious Bacteria in Bovine Milk

Prevalence and Antibiogram of Staphylococcus aureus in Clinical and Subclinical Mastitis in Holstein Dairy Cows in Egypt

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