Field-usable aptamer‑gold nanoparticles-based colorimetric sensor for rapid detection of white spot syndrome virus in shrimp

Etedali, Parisa; Behbahani, Mandana; Mohabatkar, Hassan; Dini, Ghasem

doi:10.1016/j.aquaculture.2021.737628

Cited by 7 publications

(6 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this value, aptamer DNA molecules absorb thoroughly into the negative charge citrate groups on the surface of AuNPs by their positive groups (bases). The negative groups of aptamer DNA molecules expose to the surface and make repulsion between nanoparticles [ 62 ]. In this state, the AuNPs become resistant to the 23.8 mM concentration of KCl added to the solution.…”

Section: Resultsmentioning

confidence: 99%

A label-free G-quadruplex aptamer/gold nanoparticle-based colorimetric biosensor for rapid detection of bovine viral diarrhea virus genotype 1

Rabiei,

Mohabatkar,

Behbahani

2024

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Bovine viral diarrhea virus (BVDV) is the cause of bovine viral diarrhea disease, one of the most economically important livestock diseases worldwide. The majority of BVD disease control programs rely on the detection and then elimination of persistent infection (PI) cattle, as the continuing source of disease. The main purpose of this study was to design and develop an accurate G-quadruplex-based aptasensor for rapid and simple detection of BVDV-1. In this work, we utilized in silico techniques to design a G-quadruplex aptamer specific for the detection of BVDV-1. Also, the rationally designed aptamer was validated experimentally and was used for developing a colorimetric biosensor based on an aptamer-gold nanoparticle system. Firstly, a pool of G-quadruplex forming ssDNA sequences was constructed. Then, based on the stability score in secondary and tertiary structures and molecular docking score, an aptamer (Apt31) was selected. In the experimental part, gold nanoparticles (AuNPs) with an average particle size of 31.7 nm were synthesized and electrostatically linked with the Apt31. The colorimetric test showed that salt-induced color change of AuNPs from red to purple-blue occurs only in the presence of BVDV-Apt31 complex, after 20 min. These results approved the specificity of Apt31 for BVDV. Furthermore, our biosensor could detect the virus at as low as 0.27 copies/ml, which is an acceptable value in comparison to the qPCR method. The specificity of the aptasensor was confirmed through cross-reactivity testing, while its selectivity was confirmed through plasma testing. The sample analysis showed 90% precision and 94% accuracy. It was concluded that the biosensor was adequately sensitive and specific for the detection of BVDV in plasma samples and could be used as a simple and rapid method on the farm.

show abstract

Section: Resultsmentioning

confidence: 99%

A label-free G-quadruplex aptamer/gold nanoparticle-based colorimetric biosensor for rapid detection of bovine viral diarrhea virus genotype 1

Rabiei,

Mohabatkar,

Behbahani

2024

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…199,219 They are capable of interacting with a variety of biomolecules such as oligonucleotides, aptamers, and antibodies. [220][221][222][223] Nanomaterials such as AuNPs are biologically inert making them suitable for protein immobilization without the threat of denaturation. Therefore, AuNPs are the most ubiquitous inorganic nanomaterial used for detecting viruses 170,[224][225][226][227][228][229] especially the recent SARS-CoV-2.…”

Section: Inorganic Nanomaterials In Virus Sensing and Trackingmentioning

confidence: 99%

“…246,247 AuNP-based aptasensors have also been developed for POCT applications by shrimp farmers to detect white spot syndrome virus. 223 Although the aptasensors showed lesser sensitivity than the gold-standard PCR, their colorimetric response simplifies virus recognition in the field.…”

Section: Inorganic Nanomaterials In Virus Sensing and Trackingmentioning

confidence: 99%

Nanomaterials for virus sensing and tracking

Pirzada

Altıntaş

2022

Chem. Soc. Rev.

View full text Add to dashboard Cite

show abstract

“…Currently, several tests are available for WSSV detection, including ELISA [12][13][14][15][16], immunochromatography [17], PCR assays [18][19][20][21][22][23][24][25], loop-mediated isothermal amplification [26][27][28][29][30], dark-field microscopy [31], colorimetric assays [32] and others. Among such methods, the WOAH Manual of Diagnostic Tests for Aquatic Animals recommends only two PCR protocols [18,22] suitable for WSSV detection [33].…”

Section: Introductionmentioning

confidence: 99%

In Silico Evaluation of the PCR Performance of Different Tests for Detection of WSSV

Sánchez-Paz,

Encinas-García,

Mendoza-Cano

2023

Fishes

View full text Add to dashboard Cite

In this study, the primers of different protocols for the detection of White Spot Syndrome Virus (WSSV) were analyzed in silico to evaluate their potential performance in PCR. As with any biological entity, this virus evolves constantly. Thus, this analysis showed that a few primers, including those recommended by the World Organization for Animal Health (WOAH), might mismatch with some isolates of WSSV, specially with isolates more recently sequenced. Furthermore, a set of primers recommended by WOAH, showed the potential to self-dimer and form hairpin loop structures, which could affect the efficiency of PCR, resulting in an inaccurate diagnostic result. From our perspective, and considering the evolutionary trajectory of this virus, it may be time for the WOAH to update the PCR protocols recommended for WSSV detection, which remains as a highly prevalent and lethal virus.

show abstract

Field-usable aptamer‑gold nanoparticles-based colorimetric sensor for rapid detection of white spot syndrome virus in shrimp

Cited by 7 publications

References 42 publications

A label-free G-quadruplex aptamer/gold nanoparticle-based colorimetric biosensor for rapid detection of bovine viral diarrhea virus genotype 1

A label-free G-quadruplex aptamer/gold nanoparticle-based colorimetric biosensor for rapid detection of bovine viral diarrhea virus genotype 1

Nanomaterials for virus sensing and tracking

In Silico Evaluation of the PCR Performance of Different Tests for Detection of WSSV

Contact Info

Product

Resources

About