Fifty novel HLA‐DPB1 alleles identified in a UK cohort of unrelated hematopoietic cell donors and recipients

Abstract

HLA‐DPB1 is the classical HLA class II genes with the least recorded variation on the IPD‐IMGT/HLA Database, suggesting the full extent of its diversity is perhaps yet to be characterized. Here, a full‐gene typing strategy was employed to genotype a UK cohort of 1470 HCT recipients (n = 744) and donors (n = 726). In total, 2940 full‐length HLA‐DPB1 sequences were generated, comprising 193 distinct alleles. Of these, 107 sequences contained novel variation, totaling 49 unique intronic HLA‐DPB1 alleles, and one coding variant (HLA‐DPB1*1188:01). Full‐gene sequencing resulted in zygosity changes for 129 individuals by identifying two distinct intronic variants of the same coding allele. We verified the existence of nine unconfirmed alleles and extended the sequence of two existing alleles on the IPD‐IMGT/HLA Database.

Nomenclature for factors of the HLA system, update October, November and December 2023

“…The accession number of each sequence is given, and these can be used to retrieve the sequence files from the EMBL, GenBank or DDBJ data libraries.Although accession numbers have been assigned by the data libraries and most sequences are already available, there is still the possibility that an author may not yet have allowed the sequence to be released; in such a case, you will have to contact the submitting author directly.Additional information pertaining to new sequences is often included in the publications describing these alleles; a listing of recent publica-tions that describe new human leucocyte antigen (HLA) sequences is given in Table 3. An additional 105 alleles were recently published but have not been included in Table 3 due to space considerations (Alizadeh et al, 2024;Cambridge et al, 2024). In addition, the allele DRB1*07:147Q has had a suffix change and has been renamed DRB1*07:147N.…”

“…Additional information pertaining to new sequences is often included in the publications describing these alleles; a listing of recent publica-tions that describe new human leucocyte antigen (HLA) sequences is given in Table 3. An additional 105 alleles were recently published but have not been included in Table 3 due to space considerations (Alizadeh et al, 2024;Cambridge et al, 2024). In addition, the allele DRB1*07:147Q has had a suffix change and has been renamed DRB1*07:147N.…”