Filogenetik Jenis-jenis Annonaceae dari Jawa Timur Koleksi Kebun Raya Purwodadi Berdasarkan Coding dan Non-coding sekuen DNA

Abstract

Annonaceae species of Purwodadi Botanic Garden collections from East Java encountered problems in species identification based on morphological characters. In addition, the unavailability of molecular data to support identification based on morphological characters hinders accurate identification of species. The aim of this research is to discern phylogenetic of Annonaceae species from East Java collections of Purwodadi Botanic Garden based on coding and non-coding sequence DNA. Annonaceae species used in this study are 30 species, includes outgroup from family of Magnoliaceae. Materials for DNA analysis were young leaves which were processed through DNA extraction, DNA amplification through PCR technique, DNA sequencing and data analysis with Maximum Likelihood (ML), Maximum Parsimony (MP) and Neighbour-Joining (NJ) method analysis. The results show that phylogenetic tree is divided into two subfamilies, i.e. Annonoideae and Malmeoideae. The topology of phylogenetic tree from three DNA molecular marker shows that non-coding sequence DNA (trnL-F molecular marker) has the best grouping of relationship and be able to explain the relationship between species of Annonaceae than rbcL and matK molecular marker as coding sequence DNA. Bootstrap value of trnL-F tree is very weak to high (36-100%) while rbcL is very weak to weak (17-63%) and matK tree is high (98-99%).

The diversity of endophitic fungi on Annua mutant plantation (Artemisia annua L.) based on ITS rDNA marker

“…The number of constant character and identified informative parsimony in the analyzed nucleotides will affect the bootstrap value. The more constant character and informative parsimony, the more chances of homoplasy occurring in the phylogenetic tree reconstruction process so that the bootstrap value become low [54].…”

Pengaruh Kepercayaan, Kualitas Produk, dan Kepuasan Konsumen terhadap Repurchase Intention Di Tokopedia

“…Kondisi ini berlaku sebaliknya jika produk yang dijual memiliki kualitas yang buruk atau mengalami penurunan maka berdampak pada penurunan repurchase intention. Hasil penelitian ini sejalan dengan temuan oleh (Yasa et al, 2018), (Lestari et al, 2018), (Prastiwi, 2016), (Nia & Mudiantono, 2016). Semakin baik kualitas produk yang dirasakan oleh konsumen akan mendasari keputusan konsumen untuk melakukan pembelian kembali pada periode kedepannya.…”

“…Ketika kepuasan konsumen mengalami peningkatan maka akan berdampak pada kenaikan repurchase intention. Hasil penelitian ini sejalan dengan temuan oleh (Putri et al, 2015), (N. T. , (Lestari et al, 2018), Nia dan (Jundi & Mudiantono, 2016), yang menemukan kepuasan konsumen berpengaruh positif terhadap repurchase intention. Artinya semakin tinggi rasa puas yang diperoleh konsumen maka semakin tinggi minat beli ulang pada Tokopedia.…”

Short Communication: Preliminary phylogenetic analysis of bacteria producing laccase isolated from Gunung Pancar, Bogor, Indonesia

“…Phylogenetic analysis is one of the most commonly used methods in systematics to understand the diversity of living things through reconstruction of relationships (Harnelly et al 2018). Along with rapid advancement of molecular biology, data of sequences have been used in many phylogenetic studies to attain more exact information (Lestari et al 2018). The common methods of proteinbased phylogeny are founded on multiple alignments of enzyme sequences and counting of distances (insertions, removals, and mutations) between these sequences.…”